FastQCFastQC Report
Wed 25 May 2016
SRR1779789_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779789_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123579
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT40573.282920237257139No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG6460.5227425371624629No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT2970.2403320952589032TruSeq Adapter, Index 2 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC2210.17883297323978992No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC1600.12947183582971217No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATTC207.870819E-570.0534967
TTACGCG150.002213473470.0534954
TAAACGA150.002224169769.9684312
ATAAACG150.002224169769.9684311
TCACGTG150.002224169769.968433
CGTTAAT252.387981E-455.97474724
CACAGCG252.387981E-455.9747472
TCTAACT200.00691649252.54011557
GCTAGCT200.00691649252.54011556
GCCGTCC200.00691649252.54011546
CGCATCT200.00694981852.47632615
CCATATG200.00694981852.4763269
AGCGCTA200.00694981852.4763265
GCGCTAA200.00694981852.4763266
CTGGATA200.00694981852.47632639
TATGCCG8100.047.94133443
CGTATGC8050.047.80452341
TCGTATG8000.047.66599340
GTATGCC8150.047.64721342
GCCGTCT7700.047.30884646
Produced by FastQC (version 0.11.5)