##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779789_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	123579
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.23986275985402	34.0	34.0	34.0	33.0	34.0
2	33.43781710484791	34.0	34.0	34.0	33.0	34.0
3	33.63548823020093	34.0	34.0	34.0	34.0	34.0
4	33.30701818270094	34.0	34.0	34.0	33.0	34.0
5	33.37881840765826	34.0	34.0	34.0	34.0	34.0
6	36.87228412594373	38.0	37.0	38.0	35.0	38.0
7	37.0553896697659	38.0	37.0	38.0	36.0	38.0
8	36.84871215983298	38.0	37.0	38.0	35.0	38.0
9	36.71063853891033	38.0	37.0	38.0	34.0	38.0
10-11	36.6570817048204	38.0	37.0	38.0	34.5	38.0
12-13	36.89676239490528	38.0	37.0	38.0	34.5	38.0
14-15	36.951686775261166	38.0	37.0	38.0	35.0	38.0
16-17	36.87611568308532	38.0	37.0	38.0	34.5	38.0
18-19	36.712734364252825	38.0	37.0	38.0	34.0	38.0
20-21	36.74789001367546	38.0	37.0	38.0	34.0	38.0
22-23	36.63690837440018	38.0	37.0	38.0	34.0	38.0
24-25	36.901229173241404	38.0	37.0	38.0	35.0	38.0
26-27	36.79496516398417	38.0	37.0	38.0	34.5	38.0
28-29	36.057072803631684	38.0	37.0	38.0	33.0	38.0
30-31	36.51179407504512	38.0	37.0	38.0	34.0	38.0
32-33	36.64390389952985	38.0	37.0	38.0	34.0	38.0
34-35	36.78817193859798	38.0	37.0	38.0	35.0	38.0
36-37	36.692536757863394	38.0	37.0	38.0	34.5	38.0
38-39	36.46259073143496	38.0	37.0	38.0	34.0	38.0
40-41	36.521759360409135	38.0	37.0	38.0	34.0	38.0
42-43	36.77409592244637	38.0	37.0	38.0	34.5	38.0
44-45	36.39838888484289	38.0	37.0	38.0	34.0	38.0
46-47	36.596719507359666	38.0	37.0	38.0	34.0	38.0
48-49	36.10128338957266	38.0	37.0	38.0	33.0	38.0
50-51	36.38036802369335	38.0	37.0	38.0	34.0	38.0
52-53	36.303040969744046	38.0	37.0	38.0	34.0	38.0
54-55	36.27705759069097	38.0	37.0	38.0	34.0	38.0
56-57	36.11529466980636	38.0	37.0	38.0	34.0	38.0
58-59	36.32841744956667	38.0	37.0	38.0	34.0	38.0
60-61	35.84628860890604	38.0	37.0	38.0	31.5	38.0
62-63	35.55602084496557	38.0	36.0	38.0	31.0	38.0
64-65	36.03452447422296	38.0	37.0	38.0	33.5	38.0
66-67	35.8060107299784	38.0	36.5	38.0	32.5	38.0
68-69	35.97731815276058	38.0	37.0	38.0	32.0	38.0
70-71	35.72288981137572	38.0	37.0	38.0	31.5	38.0
72-73	35.302527128395596	38.0	37.0	38.0	28.5	38.0
74-75	35.24518324310765	38.0	37.0	38.0	28.5	38.0
76	34.77979268322288	38.0	36.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	7.0
23	10.0
24	30.0
25	77.0
26	183.0
27	291.0
28	578.0
29	912.0
30	1525.0
31	2384.0
32	3729.0
33	5912.0
34	9139.0
35	14569.0
36	24032.0
37	60200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.90309315070711	18.491714630335704	11.268426548801514	29.336765670155668
2	21.17430955097549	20.28742747554196	34.098835562676506	24.439427410806044
3	19.33580948219358	21.548968675907716	31.877584379223006	27.237637462675696
4	13.817072479952094	20.12558768075482	35.9777955801552	30.07954425913788
5	13.335599090460354	34.072941195510566	37.66902143568082	14.922438278348263
6	28.785635099814694	38.96697658987369	18.283850816077166	13.963537494234457
7	28.21029462934641	28.713616391134416	21.53359389540294	21.542495084116233
8	24.865066071096223	37.184311250293334	18.442453814968562	19.50816886364188
9	23.94500683773133	17.188195405368226	20.326269026290873	38.540528730609566
10-11	24.316833766254785	26.426820090792123	27.279715809320354	21.976630333632738
12-13	24.18412513452933	22.582720365110575	27.330290745191334	25.90286375516876
14-15	22.131187337654456	23.482954223614044	22.986915252591462	31.398943186140038
16-17	24.285679605758258	28.391555199508005	23.92032626902629	23.402438925707443
18-19	24.17724694325088	26.331739211354684	25.21383082886251	24.277183016531936
20-21	21.73387064145203	25.842578431610548	25.269260958577107	27.154289968360317
22-23	22.158295503281302	28.05614222481166	25.323477289830798	24.46208498207624
24-25	24.281020893686577	27.989205198336276	25.303452070756933	22.426321837220218
26-27	23.945816036705263	28.628245899384197	25.521326438958074	21.904611624952462
28-29	23.666642390697447	26.358847376981526	26.407399315417667	23.56711091690336
30-31	24.382783482630543	27.98250511818351	26.091407116095777	21.54330428309017
32-33	21.757337411696163	28.45103132409228	25.713915794754772	24.077715469456784
34-35	23.727736913229595	26.07158174123435	26.157761431958505	24.04291991357755
36-37	22.686297833774347	27.562530850710882	27.6818876993664	22.069283616148375
38-39	21.742367230678354	26.16059362836728	28.40490698257795	23.692132158376424
40-41	21.420306039051944	24.964192945403347	29.08787091657968	24.527630098965034
42-43	24.016815290747545	25.119762417258734	28.6426386573662	22.220783634627523
44-45	21.33817234319747	27.44115100462053	29.073709934535803	22.1469667176462
46-47	23.614853656365565	24.320475161637496	27.484443149726083	24.580228032270853
48-49	22.959807086964616	24.770794390632712	27.24492025344112	25.024478268961552
50-51	21.106304454273587	24.685667574490815	29.181218157022553	25.026809814213046
52-53	20.586045005666183	24.535777885705034	31.788084830824026	23.09009227780476
54-55	19.907379295548296	24.87764594440329	30.192406559553735	25.02256820049468
56-57	22.254248678233974	24.861346136718783	27.771255536033813	25.113149649013433
58-59	20.399085260963716	24.855807176249165	31.083722906927324	23.661384655859795
60-61	21.974526159861096	27.100863293629114	27.752886751903254	23.17172379460654
62-63	19.605883019539913	30.22494374382801	26.501513655275126	23.667659581356947
64-65	19.61872157205523	30.764094595250814	25.552372165298888	24.06481166739507
66-67	19.520378840004856	30.292224875541347	25.85340186991541	24.33399441453839
68-69	19.736341639084237	30.615688400812502	25.38338903770363	24.26458092239963
70-71	20.10026867797488	30.439515084811603	24.787566360222712	24.672649876990807
72-73	20.24783689062639	28.88489587127375	24.893766845543063	25.973500392556797
74-75	20.579713663696474	28.645770105453987	24.62386999134031	26.15064623950923
76	20.72799663354158	28.017673906710254	24.919884763538665	26.3344446962095
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	2.0
14	3.0
15	5.0
16	10.5
17	14.0
18	21.5
19	50.5
20	82.5
21	93.0
22	119.5
23	174.0
24	264.0
25	326.0
26	389.5
27	558.0
28	786.5
29	910.0
30	1035.5
31	1310.0
32	1783.0
33	2107.0
34	2434.0
35	2988.0
36	3628.0
37	4041.0
38	4320.0
39	5255.5
40	6318.0
41	7342.5
42	7961.0
43	8612.0
44	9410.5
45	9017.0
46	8476.0
47	8161.5
48	7299.5
49	6564.5
50	6377.0
51	5773.5
52	4701.0
53	3986.0
54	3740.0
55	3440.5
56	2812.5
57	2188.5
58	1893.0
59	1736.0
60	1449.5
61	1201.0
62	1082.0
63	1064.0
64	922.0
65	767.5
66	700.5
67	664.0
68	634.5
69	589.5
70	554.0
71	534.0
72	485.5
73	438.0
74	394.0
75	349.0
76	334.0
77	292.0
78	262.5
79	260.0
80	231.5
81	185.5
82	149.5
83	131.0
84	118.0
85	87.0
86	52.0
87	35.0
88	29.5
89	24.5
90	18.5
91	7.5
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03884155074891365
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	8.091989739357011E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.091989739357011E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.019016175887488974
52-53	0.03155875998349234
54-55	0.052193333818852716
56-57	0.05664392817549907
58-59	0.037627752288010095
60-61	0.032772558444395895
62-63	0.02994036203562094
64-65	0.01699317845264972
66-67	0.03560475485317085
68-69	0.00809198973935701
70-71	0.008901188713292712
72-73	0.02427596921807103
74-75	0.014565581530842621
76	0.005664392817549907
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	123579.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.61382597366867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	51.10836077530002	18.712726272263087
2	18.29513558909983	13.397098212479467
3	11.494684730479369	12.625931590318743
4	6.643534377969811	9.72980846260287
5	3.684222158375141	6.744673447754068
6	2.254293102305125	4.952297720486491
7	1.3790969567043119	3.5345811181511424
8	0.9326585187968263	2.7318557360069264
9	0.7359603951643203	2.425169324885296
>10	3.427851570269852	19.949991503410775
>50	0.03094127787477623	0.7501274488383949
>100	0.008840365107078923	0.6400763883831395
>500	0.002210091276769731	0.5227425371624629
>1k	0.002210091276769731	3.282920237257139
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT	4057	3.282920237257139	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	646	0.5227425371624629	No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT	297	0.2403320952589032	TruSeq Adapter, Index 2 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	221	0.17883297323978992	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	160	0.12947183582971217	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0016183979478714022	0.0
2	0.0	0.0	0.0	0.0016183979478714022	0.0
3	0.0	0.0	0.0	0.0016183979478714022	0.0
4	0.0	0.0	0.0	0.004855193843614206	0.0
5	0.0	0.0	0.0	0.005664392817549907	0.0
6	0.0	0.0	0.0	0.005664392817549907	0.0
7	0.0	0.0	0.0	0.005664392817549907	0.0
8	0.0	0.0	0.0	0.005664392817549907	0.0
9	0.0	0.0	0.0	0.005664392817549907	0.0
10	0.0	0.0	0.0	0.00728279076542131	0.0
11	0.0	0.0	0.0	0.00809198973935701	0.0
12	0.0	0.0	0.0	0.00809198973935701	0.0
13	0.0	0.0	0.0	0.00809198973935701	0.0
14	0.0	0.0	0.0	0.00809198973935701	0.0
15	0.0	0.0	0.0	0.00809198973935701	0.0
16	0.0	0.0	0.0	0.00809198973935701	0.0
17	0.0	0.0	0.0	0.008901188713292712	0.0
18	0.0	0.0	0.0	0.008901188713292712	0.0
19	0.0	0.0	0.0	0.011328785635099814	0.0
20	0.0	0.0	0.0	0.011328785635099814	0.0
21	0.0	0.0	0.0	0.012947183582971217	0.0
22	0.0	0.0	0.0	0.013756382556906917	0.0
23	0.0	0.0	0.0	0.013756382556906917	0.0
24	0.0	0.0	0.0	0.013756382556906917	0.0
25	0.0	0.0	0.0	0.01618397947871402	0.0
26	0.0	0.0	0.0	0.017802377426585424	0.0
27	0.0	0.0	0.0	0.02508516819200673	0.0
28	0.0	0.0	0.0	0.02832196408774954	0.0
29	0.0	0.0	0.0	0.042887545618592156	0.0
30	0.0	0.0	0.0	0.05826232612337048	0.0
31	0.0	0.0	0.0	0.078492300471763	0.0
32	0.0	0.0	0.0	0.09143948405473422	0.0
33	0.0	0.0	0.0	0.11652465224674095	0.0
34	0.0	0.0	0.0	0.1521294070999118	0.0
35	0.0	0.0	0.0	0.20877333527541087	0.0
36	0.0	0.0	0.0	0.3074956100955664	0.0
37	0.0	0.0	0.0	0.49118377717897055	0.0
38	0.0	0.0	0.0	0.7064307042458671	0.0
39	0.0	0.0	0.0	1.0114987174196264	0.0
40	0.0	0.0	0.0	1.369973862873142	0.0
41	0.0	0.0	0.0	1.7753825488149282	0.0
42	0.0	0.0	0.0	2.225297178323178	0.0
43	0.0	0.0	0.0	2.6841129965447204	0.0
44	0.0	0.0	0.0	3.1890531562805977	0.0
45	0.0	0.0	0.0	3.7482096472701674	0.0
46	0.0	0.0	0.0	4.312221332103351	0.0
47	0.0	0.0	0.0	4.929640149216291	0.0
48	0.0	0.0	0.0	5.524401395059031	0.0
49	0.0	0.0	0.0	6.028532355820973	0.0
50	0.0	0.0	0.0	6.640286780116363	0.0
51	0.0	0.0	0.0	7.191351281366575	0.0
52	0.0	0.0	0.0	7.787730925157187	0.0
53	0.0	0.0	0.0	8.38006457407812	0.0
54	0.0	0.0	0.0	8.952977447624596	0.0
55	0.0	0.0	0.0	9.495140760161517	0.0
56	0.0	0.0	0.0	10.046205261411728	0.0
57	0.0	0.0	0.0	10.602124956505556	0.0
58	0.0	0.0	0.0	11.164518243390868	0.0
59	0.0	0.0	0.0	11.747950703598509	0.0
60	0.0	0.0	0.0	12.354040735076348	0.0
61	0.0	0.0	0.0	12.949611179893024	0.0
62	0.0	0.0	0.0	13.571075991875642	0.0
63	0.0	0.0	0.0	14.133469278760954	0.0
64	0.0	0.0	0.0	14.76869047330048	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGATTC	20	7.870819E-5	70.05349	67
TTACGCG	15	0.0022134734	70.05349	54
TAAACGA	15	0.0022241697	69.96843	12
ATAAACG	15	0.0022241697	69.96843	11
TCACGTG	15	0.0022241697	69.96843	3
CGTTAAT	25	2.387981E-4	55.974747	24
CACAGCG	25	2.387981E-4	55.974747	2
TCTAACT	20	0.006916492	52.540115	57
GCTAGCT	20	0.006916492	52.540115	56
GCCGTCC	20	0.006916492	52.540115	46
CGCATCT	20	0.006949818	52.476326	15
CCATATG	20	0.006949818	52.476326	9
AGCGCTA	20	0.006949818	52.476326	5
GCGCTAA	20	0.006949818	52.476326	6
CTGGATA	20	0.006949818	52.476326	39
TATGCCG	810	0.0	47.941334	43
CGTATGC	805	0.0	47.804523	41
TCGTATG	800	0.0	47.665993	40
GTATGCC	815	0.0	47.647213	42
GCCGTCT	770	0.0	47.308846	46
>>END_MODULE