##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779788_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21268
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.093285687417715	31.0	21.0	34.0	12.0	34.0
2	26.398203874365244	32.0	21.0	34.0	12.0	34.0
3	25.969625728794433	32.0	21.0	34.0	11.0	34.0
4	26.57617077299229	32.0	21.0	34.0	12.0	34.0
5	26.04908783148392	32.0	12.0	34.0	11.0	34.0
6	28.873659958623282	34.0	21.0	38.0	11.0	38.0
7	28.496097423359036	34.0	21.0	38.0	12.0	38.0
8	27.939580590558585	34.0	11.0	38.0	11.0	38.0
9	28.953263118299795	35.0	21.0	38.0	11.0	38.0
10-11	27.82544197855934	33.0	16.5	38.0	11.0	38.0
12-13	28.37932574760203	34.0	21.0	38.0	11.0	38.0
14-15	27.344249576829036	32.5	11.5	38.0	11.0	38.0
16-17	27.634380289637015	32.5	16.0	38.0	11.0	38.0
18-19	27.913790671431258	34.0	11.5	38.0	11.0	38.0
20-21	27.527694188452134	32.5	16.0	38.0	11.0	38.0
22-23	27.94945457965018	34.0	16.0	38.0	11.0	38.0
24-25	28.16287380101561	34.0	21.0	38.0	11.0	38.0
26-27	27.30538837690427	32.5	11.0	38.0	11.0	38.0
28-29	27.933115478653377	34.0	11.0	38.0	11.0	38.0
30-31	28.129725409065262	34.5	11.0	38.0	11.0	38.0
32-33	28.259921008087268	35.0	11.0	38.0	11.0	38.0
34-35	28.421172653752116	34.5	16.0	38.0	11.0	38.0
36-37	28.08475173970284	34.0	11.0	38.0	11.0	38.0
38-39	27.968732367876623	34.0	11.0	38.0	11.0	38.0
40-41	27.90398721083318	34.0	11.0	38.0	11.0	38.0
42-43	28.144089712243748	34.0	11.0	38.0	11.0	38.0
44-45	28.313217039684034	34.0	16.0	38.0	11.0	38.0
46-47	28.11973387248448	34.0	11.0	38.0	11.0	38.0
48-49	27.946703968403234	34.0	11.0	38.0	11.0	38.0
50-51	27.488339289072783	33.5	11.0	38.0	11.0	38.0
52-53	27.93497272898251	34.0	11.0	38.0	11.0	38.0
54-55	27.921337220236975	34.0	11.0	38.0	11.0	38.0
56-57	27.970025390257664	34.0	11.0	38.0	11.0	38.0
58-59	28.03902576640963	34.0	11.0	38.0	11.0	38.0
60-61	28.09521346624036	34.0	11.0	38.0	11.0	38.0
62-63	27.95248730487117	34.0	11.0	38.0	11.0	38.0
64-65	27.953827346247884	34.0	11.0	38.0	11.0	38.0
66-67	28.044621026894866	34.0	16.0	38.0	11.0	38.0
68-69	27.6673641151025	33.5	15.0	38.0	11.0	38.0
70-71	27.6667293586609	34.0	14.5	38.0	11.0	38.0
72-73	27.609436712431823	34.0	11.0	38.0	11.0	38.0
74-75	27.66792834305059	34.0	11.0	38.0	11.0	38.0
76	27.075371450065827	32.0	11.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	8.0
13	112.0
14	509.0
15	1014.0
16	1388.0
17	1474.0
18	1394.0
19	1203.0
20	868.0
21	596.0
22	432.0
23	300.0
24	227.0
25	196.0
26	175.0
27	168.0
28	155.0
29	215.0
30	259.0
31	328.0
32	385.0
33	532.0
34	809.0
35	1233.0
36	2292.0
37	4996.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.979499717886025	22.31521534700019	9.892796689862704	40.81248824525108
2	14.354899379349256	22.14594696257288	37.97724280609366	25.521910851984202
3	12.63400413767162	29.739514763964642	33.09667105510626	24.529810043257477
4	10.466428437088583	27.75531314651119	25.07993229264623	36.698326123754
5	10.739138611999248	30.101561030656388	29.60315967650931	29.556140680835057
6	20.335715629114162	44.183750235094976	13.80007523039308	21.68045890539778
7	18.60071468873425	29.885273650554822	21.990784276847847	29.52322738386308
8	16.047583223622343	38.86590182433704	21.045702463795372	24.04081248824525
9	19.284303583184425	20.77024358130349	18.884604533057463	41.06084830245462
10-11	17.559656753262022	30.72528505936288	24.215352063006932	27.499706124368167
12-13	17.27477901072033	23.401354147075416	27.51786721835622	31.805999623848034
14-15	24.887154410381797	27.586044762083883	20.660146699266505	26.866654128267818
16-17	26.431728418280986	25.801673876246	20.140586797066014	27.626010908406997
18-19	19.856588691665685	25.44727871164923	23.829787234042556	30.866345362642527
20-21	24.228888470942263	24.447526800827536	22.371638141809292	28.951946586420913
22-23	26.65036674816626	24.134850479593755	20.55200300921572	28.66277976302426
24-25	19.91301281297755	30.682966968378985	20.420829904784295	28.983190313859176
26-27	22.632593567801393	30.94555200300921	22.122437464735754	24.29941696445364
28-29	20.876924885388505	28.53179734336429	24.892441518749266	25.69883625249794
30-31	22.5032913296972	27.172277600150462	24.84953921384239	25.47489185630995
32-33	18.54429189392515	29.43154034229829	24.952981004325746	27.071186759450818
34-35	17.73086326876058	32.69936054165883	23.742241865713748	25.827534323866843
36-37	23.245314961555643	26.367890145547747	24.11766089021609	26.269134002680524
38-39	24.322290927044882	24.296428655396987	25.85286718547951	25.528413232078623
40-41	20.279769601504643	29.140707652521453	24.452803573527685	26.126719172446222
42-43	16.685082872928177	27.868813917949925	25.8634066063242	29.582696602797697
44-45	16.69723045093337	24.35228287957869	29.64216861804674	29.3083180514412
46-47	18.575290936875515	27.50675913953215	26.674503350182206	27.243446573410136
48-49	19.348771599858942	28.139179499235922	25.092276948395437	27.4197719525097
50-51	25.2110126254908	27.524510380175393	21.171796017210166	26.092680977123646
52-53	15.111685868798494	30.90289207618152	24.60380907594639	29.381612979073594
54-55	17.804579865519347	26.484694597263363	24.469835895989092	31.240889641228193
56-57	19.616279889962616	28.207660294843762	22.428346382638544	29.747713432555077
58-59	18.101664628985233	29.079281482178125	22.893350888742596	29.925703000094046
60-61	16.412716328066214	25.8864747930775	24.266365688487586	33.4344431903687
62-63	18.66080455175981	27.324665553805282	23.48999600310347	30.524533891331433
64-65	19.40603837471783	26.274454477050412	22.690933032355154	31.6285741158766
66-67	16.41703308330786	31.99228761550942	20.618871828634582	30.971807472548146
68-69	17.780651228400142	33.89679087810038	18.911484659692018	29.411073233807457
70-71	18.74412262554072	32.751081436900506	18.52783524543916	29.976960692119615
72-73	20.147639646417154	31.509779951100242	19.24017302990408	29.10240737257852
74-75	17.92297564186965	31.385780118499014	20.394056240007526	30.297187999623816
76	16.729358660899003	30.275531314651122	20.923453075042318	32.07165694940756
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	3.0
16	8.0
17	11.0
18	13.5
19	18.5
20	32.0
21	43.0
22	38.0
23	49.5
24	69.0
25	72.0
26	96.5
27	140.0
28	157.0
29	155.0
30	175.5
31	207.0
32	284.0
33	350.0
34	367.0
35	457.0
36	604.0
37	678.0
38	669.0
39	713.0
40	757.0
41	834.5
42	921.0
43	962.5
44	1048.5
45	1107.5
46	1122.0
47	1121.5
48	1149.0
49	1166.5
50	1156.0
51	1108.5
52	1029.0
53	949.5
54	902.0
55	868.5
56	790.0
57	732.5
58	720.0
59	645.0
60	520.5
61	436.0
62	401.0
63	344.0
64	275.5
65	234.5
66	195.5
67	186.0
68	176.0
69	146.0
70	111.0
71	96.0
72	89.5
73	67.0
74	51.5
75	52.0
76	50.5
77	42.0
78	35.5
79	36.0
80	25.0
81	18.0
82	17.0
83	12.0
84	14.5
85	14.5
86	9.5
87	7.0
88	5.5
89	3.5
90	4.0
91	2.5
92	0.0
93	1.0
94	1.5
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.00940379913485048
10-11	0.00235094978371262
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.00235094978371262
20-21	0.0
22-23	0.0
24-25	0.00235094978371262
26-27	0.0
28-29	0.00235094978371262
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.016456648485988337
38-39	0.00705284935113786
40-41	0.00235094978371262
42-43	0.00235094978371262
44-45	0.00470189956742524
46-47	0.00235094978371262
48-49	0.00235094978371262
50-51	0.00705284935113786
52-53	0.01410569870227572
54-55	0.00470189956742524
56-57	0.0117547489185631
58-59	0.00940379913485048
60-61	0.01880759826970096
62-63	0.00705284935113786
64-65	0.01880759826970096
66-67	0.016456648485988337
68-69	0.00235094978371262
70-71	0.0
72-73	0.0
74-75	0.00940379913485048
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	21268.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.69362422418658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.62262718299165	78.56403987210832
2	5.640525002711791	9.7799511002445
3	2.2562100010847166	5.8679706601467
4	0.9653975485410564	3.3477524920067703
5	0.3199913222692266	1.3870603723904458
6	0.16270745200130166	0.8463419221365431
7	0.02169432693350689	0.1316531878879067
8	0.010847163466753445	0.07523039307880384
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0047018995674252394	0.0
2	0.0	0.0	0.0	0.0047018995674252394	0.0
3	0.0	0.0	0.0	0.0047018995674252394	0.0
4	0.0	0.0	0.0	0.01410569870227572	0.0
5	0.0	0.0	0.0	0.018807598269700958	0.0
6	0.0	0.0	0.0	0.018807598269700958	0.0
7	0.0	0.0	0.0	0.018807598269700958	0.0
8	0.0	0.0	0.0	0.018807598269700958	0.0
9	0.0	0.0	0.0	0.018807598269700958	0.0
10	0.0	0.0	0.0	0.018807598269700958	0.0
11	0.0	0.0	0.0	0.0235094978371262	0.0
12	0.0	0.0	0.0	0.0235094978371262	0.0
13	0.0	0.0	0.0	0.02821139740455144	0.0
14	0.0	0.0	0.0	0.02821139740455144	0.0
15	0.0	0.0	0.0	0.02821139740455144	0.0
16	0.0	0.0	0.0	0.02821139740455144	0.0
17	0.0	0.0	0.0	0.02821139740455144	0.0
18	0.0	0.0	0.0	0.02821139740455144	0.0
19	0.0	0.0	0.0	0.02821139740455144	0.0
20	0.0	0.0	0.0	0.02821139740455144	0.0
21	0.0	0.0	0.0	0.02821139740455144	0.0
22	0.0	0.0	0.0	0.08463419221365431	0.0
23	0.0	0.0	0.0	0.12695128832048147	0.0
24	0.0	0.0	0.0	0.13635508745533195	0.0
25	0.0	0.0	0.0	0.13635508745533195	0.0
26	0.0	0.0	0.0	0.13635508745533195	0.0
27	0.0	0.0	0.0	0.1410569870227572	0.0
28	0.0	0.0	0.0	0.16926838442730863	0.0
29	0.0	0.0	0.0	0.1786721835621591	0.0
30	0.0	0.0	0.0	0.2021816813992853	0.0
31	0.0	0.0	0.0	0.21158548053413578	0.0
32	0.0	0.0	0.0	0.22569117923641152	0.0
33	0.0	0.0	0.0	0.25860447620838817	0.0
34	0.0	0.0	0.0	0.2962196727477901	0.0
35	0.0	0.0	0.0	0.36674816625916873	0.0
36	0.0	0.0	0.0	0.47959375587737446	0.0
37	0.0	0.0	0.0	0.6206507429001317	0.0
38	0.0	0.0	0.0	0.8369381230016927	0.0
39	0.0	0.0	0.0	1.137859695316908	0.0
40	0.0	0.0	0.0	1.391762271957871	0.0
41	0.0	0.0	0.0	1.66917434643596	0.0
42	0.0	0.0	0.0	1.9794997178860259	0.0
43	0.0	0.0	0.0	2.4214782772239984	0.0
44	0.0	0.0	0.0	2.764716945646041	0.0
45	0.0	0.0	0.0	3.2019936054165883	0.0
46	0.0	0.0	0.0	3.6533759638894114	0.0
47	0.0	0.0	0.0	3.9307880383675005	0.0
48	0.0	0.0	0.0	4.269324807222118	0.0
49	0.0	0.0	0.0	4.5185254842956555	0.0
50	0.0	0.0	0.0	4.955802144066203	0.0
51	0.0	0.0	0.0	5.209704720707165	0.0
52	0.0	0.0	0.0	5.567049087831484	0.0
53	0.0	0.0	0.0	5.905585856686101	0.0
54	0.0	0.0	0.0	6.2723340229452695	0.0
55	0.0	0.0	0.0	6.577957494827911	0.0
56	0.0	0.0	0.0	6.907090464547677	0.0
57	0.0	0.0	0.0	7.269136731239421	0.0
58	0.0	0.0	0.0	7.602971600526613	0.0
59	0.0	0.0	0.0	7.880383675004702	0.0
60	0.0	0.0	0.0	8.294150836938122	0.0
61	0.0	0.0	0.0	8.585668610118487	0.0
62	0.0	0.0	0.0	8.924205378973106	0.0
63	0.0	0.0	0.0	9.267444047395148	0.0
64	0.0	0.0	0.0	9.540154222305812	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGT	15	0.0021948926	70.00001	42
TGCCGCT	15	0.0021948926	70.00001	13
GGTTCAA	15	0.0021948926	70.00001	14
GCCGCTG	15	0.0021948926	70.00001	14
GGGTTCA	25	2.3378496E-4	55.999996	13
CATCTGC	25	2.3378496E-4	55.999996	9
CTCTTCC	25	2.3378496E-4	55.999996	16
TCTGCCG	25	2.3378496E-4	55.999996	11
CCTCACG	25	2.3378496E-4	55.999996	11
ATCTGCC	25	2.3378496E-4	55.999996	10
TCTCGGT	20	0.0068589775	52.499996	43
CTGTGTA	20	0.0068589775	52.499996	34
AGCATTA	20	0.0068589775	52.499996	59
TTTATAC	20	0.0068589775	52.499996	3
TGCAGAA	20	0.0068589775	52.499996	55
CAGTGCA	20	0.0068589775	52.499996	52
CATACCT	20	0.0068589775	52.499996	7
TAGACCC	20	0.0068589775	52.499996	49
CTTCCGA	20	0.0068589775	52.499996	18
CCGAAGA	20	0.0068589775	52.499996	21
>>END_MODULE