FastQCFastQC Report
Wed 25 May 2016
SRR1779788_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779788_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21268
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT558526.260109084069967No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG8303.902576640962949No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC4071.9136731239420728No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT2791.311829979311642RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC1640.7711115290577393No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT1080.5078051532819259No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT1070.5031032537145007No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC910.4278728606356968No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC840.39495956366372015No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT810.3808538649614444No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAGCTCGTATGCCGT480.22569117923641152No Hit
CTTATACACATCTCCGAGCCCACGAGACCATGGTAGATCTCGTATGCCGT410.19277788226443482No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC400.1880759826970096No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCG350.1645664848598834No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCG340.15986458529245814No Hit
CCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG320.1504607861576077No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG310.14575888659018243No Hit
CTTATACACCTCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT290.13635508745533195No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG280.1316531878879067No Hit
TCTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT250.11754748918563099No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT230.1081436900507805No Hit
CTTATACACATCTCCGAGCCCACGAGAAAATGGTAGATCTCGTATGCCGT220.10344179048335528No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACAC150.002194892670.000013
GAGTTGC150.002194892670.0000129
AGTTGCA150.002194892670.0000130
TGAAAAA8750.053.259
TCTCGTA8700.052.70115338
GCTTGAA9050.052.59668456
TGCTTGA9000.052.555
TTGAAAA9000.052.558
CTTGAAA9000.052.557
CTACACA200.006858977552.4999964
GTAGAGC200.006858977552.49999633
TGTTGAA200.006858977552.49999640
GTACATT200.006858977552.49999661
GGTAGAG200.006858977552.49999632
ATCTCGT8750.052.437
AGATCTC8700.052.2988535
GAAAAAA8650.052.1965360
GATCTCG8650.052.1965336
CTCGTAT9350.052.0320839
CTTCTGC9100.051.9230851
Produced by FastQC (version 0.11.5)