FastQCFastQC Report
Wed 25 May 2016
SRR1779787_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779787_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40682
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGAAC150.00220945570.0000128
CTCGGTG309.996256E-870.0000144
AGTTGTA150.00220945570.0000126
TAGTGTA150.00220945570.0000134
TCGGTGG309.996256E-870.0000145
TCTCGGT353.594323E-970.043
CGACGAA352.904635E-760.022
GATCTCG352.904635E-760.041
CGTATCA252.36361E-455.99999656
TGGTCGC252.36361E-455.99999649
GTGTAGA700.055.036
GGTGGTC452.6178895E-854.44444747
CGGTGGT452.6178895E-854.44444746
ATCTCGG407.30568E-752.49999642
GTCGCCG200.00690413552.49999651
GGTCGAC200.00690413552.49999650
ATGCTTC200.00690413552.4999966
CTAGTGT200.00690413552.49999633
ACGTGTA200.00690413552.49999634
CAGGCAT200.00690413552.49999627
Produced by FastQC (version 0.11.5)