##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779787_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40682
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.928567917014895	34.0	23.0	34.0	12.0	34.0
2	29.576471166609313	34.0	31.0	34.0	12.0	34.0
3	29.12349442013667	34.0	27.0	34.0	11.0	34.0
4	29.363698933189127	34.0	27.0	34.0	12.0	34.0
5	29.702227029152944	34.0	31.0	34.0	11.0	34.0
6	32.4909050685807	38.0	31.0	38.0	12.0	38.0
7	32.63548989725186	38.0	32.0	38.0	12.0	38.0
8	32.06521311636596	37.0	28.0	38.0	11.0	38.0
9	32.66887075365026	38.0	34.0	38.0	12.0	38.0
10-11	31.396637333464433	37.0	27.0	38.0	11.5	38.0
12-13	32.14324516985399	37.0	29.5	38.0	11.0	38.0
14-15	30.97065041050096	37.0	25.5	38.0	11.0	38.0
16-17	31.519922815987414	37.0	28.5	38.0	11.0	38.0
18-19	32.19080920308736	37.5	31.0	38.0	11.0	38.0
20-21	31.21435770119463	37.0	27.0	38.0	11.0	38.0
22-23	31.96236664864068	37.5	29.0	38.0	11.0	38.0
24-25	32.22540681382429	38.0	31.0	38.0	11.0	38.0
26-27	31.23619782704882	37.0	26.0	38.0	11.0	38.0
28-29	32.318617570424266	37.5	32.0	38.0	11.0	38.0
30-31	32.56661422742245	38.0	33.5	38.0	11.0	38.0
32-33	32.72137554692493	38.0	34.0	38.0	11.0	38.0
34-35	32.74147042918244	38.0	34.0	38.0	11.0	38.0
36-37	32.37004080428691	38.0	32.0	38.0	11.0	38.0
38-39	32.29882994936335	38.0	32.5	38.0	11.0	38.0
40-41	32.277456860528	38.0	31.5	38.0	11.0	38.0
42-43	32.57149353522443	38.0	32.5	38.0	11.0	38.0
44-45	32.60857381642987	38.0	33.5	38.0	11.0	38.0
46-47	32.513310555036625	38.0	33.0	38.0	11.0	38.0
48-49	32.34194484046999	38.0	32.5	38.0	11.0	38.0
50-51	31.80783884764761	37.5	28.5	38.0	11.0	38.0
52-53	32.33614374907822	38.0	31.0	38.0	11.0	38.0
54-55	32.41857578290153	38.0	32.0	38.0	11.0	38.0
56-57	32.37742736345312	38.0	32.0	38.0	11.0	38.0
58-59	32.50319551644462	38.0	33.5	38.0	11.0	38.0
60-61	32.49916424954526	38.0	32.5	38.0	11.0	38.0
62-63	32.43327515854678	38.0	32.0	38.0	11.0	38.0
64-65	32.49972961014699	38.0	32.5	38.0	11.0	38.0
66-67	32.39544516002164	38.0	31.0	38.0	11.0	38.0
68-69	31.850339216361043	37.0	28.5	38.0	11.0	38.0
70-71	31.873076544909296	37.0	29.0	38.0	11.0	38.0
72-73	32.03370040804287	37.5	31.0	38.0	11.0	38.0
74-75	32.185044983039184	37.5	31.0	38.0	11.0	38.0
76	31.360970453763336	37.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	14.0
13	156.0
14	573.0
15	1098.0
16	1323.0
17	1422.0
18	1310.0
19	976.0
20	681.0
21	543.0
22	362.0
23	302.0
24	245.0
25	258.0
26	284.0
27	271.0
28	345.0
29	415.0
30	541.0
31	650.0
32	924.0
33	1254.0
34	1915.0
35	3283.0
36	6468.0
37	15069.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.57996165380266	21.097782803205348	10.877046359569341	34.44520918342264
2	16.3635022860233	22.235878275404357	35.81928125460892	25.581338183963425
3	21.43945725382233	21.29934614817364	31.969912983629122	25.29128361437491
4	13.0696622584927	22.112973796765154	34.40342166068531	30.413942284056834
5	13.56373826262229	32.38041394228406	36.903298756206674	17.152549038886978
6	24.748045818789638	41.22707831473379	17.327073398554642	16.69780246792193
7	22.585482166121775	34.32314839851528	20.085543619871686	23.005825815491264
8	19.871196106386115	38.02910378054176	17.48439113121282	24.6153089818593
9	23.28851306506723	18.12393992281409	20.464098719303852	38.12344829281483
10-11	21.81143299615295	27.58815648775212	24.002900652646847	26.59750986344809
12-13	21.761466987857037	23.399783688117594	26.374072071186273	28.464677252839092
14-15	24.209724202349932	25.570276780885898	22.772970847057667	27.447028169706506
16-17	25.469495108401752	26.622339118037463	22.64637923405929	25.2617865395015
18-19	21.955925912269706	26.512050932241927	24.938854270368598	26.593168885119773
20-21	23.73039673565705	25.46334988446979	24.845140356914605	25.961113022958553
22-23	25.255641315569537	25.894744604493386	23.1527456860528	25.696868393884277
24-25	21.879724198960215	28.805476690879146	23.220628541228812	26.094170568931823
26-27	22.943808072366156	28.860429674057325	24.599331399636203	23.596430853940316
28-29	22.23000626820545	27.74971424357509	25.58288165382299	24.43739783439647
30-31	22.737328548252297	26.817757239073792	25.902118873211737	24.54279533946217
32-33	20.559461186765645	29.01037313799715	25.227373285482525	25.20279238975468
34-35	20.155600894766597	30.30776037953836	24.508984538236522	25.02765418745852
36-37	23.140272383106346	26.91012340823049	24.471458773784356	25.4781454348788
38-39	23.400646501395016	25.68798318604737	26.11201927213284	24.799351040424774
40-41	21.374317880143554	27.832948232633598	25.376087704635957	25.416646182586895
42-43	19.97074873408387	27.03038198711961	26.32859741408977	26.670271864706752
44-45	19.795481920306777	25.34967183697549	28.355989282466016	26.498856960251715
46-47	20.23868049751733	26.65183619291087	27.544122707831477	25.565360601740327
48-49	20.364043065729316	27.60926208151025	26.532618848630847	25.49407600412959
50-51	23.087606312374024	27.01809153925569	24.58089572784032	25.313406420529965
52-53	18.54241954913096	28.642721931312536	26.20522654079701	26.609631978759495
54-55	19.381760078662733	26.44911504424779	26.371681415929203	27.797443461160277
56-57	20.58407797539301	27.51385833159208	25.595201514276233	26.306862178738676
58-59	20.549410029498524	26.889134709931174	25.581366764995085	26.98008849557522
60-61	19.17616255485489	26.724932699044878	25.870610072402307	28.228294673697928
62-63	20.301130776794494	26.680186823992138	25.432645034414946	27.586037364798425
64-65	20.949442552825342	26.23873735449215	24.8976681867909	27.91415190589161
66-67	19.327162997652323	28.613395282520248	23.959831360546726	28.099610359280703
68-69	20.11577601887813	30.02925126591613	23.23754977631385	26.617422938891895
70-71	20.489405633941303	28.76702227029153	23.31743768742933	27.426134408337838
72-73	21.05282564342076	28.230623632654066	23.427398539858903	27.289152184066275
74-75	20.14749262536873	27.860127826941987	24.39282202556539	27.599557522123895
76	19.633244020550134	27.376416508935375	24.269314913596027	28.72102455691846
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	0.5
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	5.0
16	13.5
17	19.0
18	29.5
19	37.0
20	48.5
21	63.0
22	76.0
23	100.5
24	137.5
25	163.0
26	222.5
27	290.5
28	370.0
29	441.0
30	479.0
31	579.5
32	758.0
33	874.0
34	938.5
35	1094.0
36	1260.5
37	1336.0
38	1427.0
39	1576.5
40	1688.5
41	1801.0
42	1860.0
43	1931.5
44	1957.0
45	1990.5
46	2070.0
47	2101.5
48	2034.0
49	1940.0
50	1945.0
51	1865.0
52	1668.5
53	1508.0
54	1464.0
55	1424.5
56	1320.5
57	1192.0
58	1128.0
59	1015.0
60	856.0
61	747.5
62	685.0
63	653.5
64	589.0
65	503.5
66	397.5
67	344.0
68	345.0
69	320.0
70	260.5
71	227.0
72	211.0
73	164.0
74	140.0
75	147.0
76	129.0
77	97.5
78	78.5
79	73.0
80	72.5
81	63.0
82	50.5
83	47.0
84	38.0
85	25.5
86	18.5
87	15.0
88	14.0
89	10.0
90	4.0
91	0.5
92	0.0
93	1.5
94	2.0
95	2.0
96	3.0
97	1.5
98	0.0
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.002458089572784032
8	0.0
9	0.002458089572784032
10-11	0.0036871343591760486
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.001229044786392016
20-21	0.0
22-23	0.0
24-25	0.001229044786392016
26-27	0.0
28-29	0.001229044786392016
30-31	0.0
32-33	0.0
34-35	0.002458089572784032
36-37	0.009832358291136128
38-39	0.0036871343591760486
40-41	0.0
42-43	0.0
44-45	0.002458089572784032
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.01229044786392016
54-55	0.004916179145568064
56-57	0.00614522393196008
58-59	0.004916179145568064
60-61	0.01597758222309621
62-63	0.004916179145568064
64-65	0.013519492650312177
66-67	0.008603313504744114
68-69	0.0
70-71	0.0
72-73	0.002458089572784032
74-75	0.004916179145568064
76	0.002458089572784032
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	40682.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.16926404798191
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.67165700452315	63.025416646182585
2	11.129515193986112	17.17712993461482
3	4.484933426769446	10.382970355439753
4	1.831560170733261	5.653606017403274
5	0.5988405427788749	2.31060419841699
6	0.17519271198318148	0.8111695590187306
7	0.0700770847932726	0.37854579420874096
8	0.01911193221634707	0.11798829949363356
9	0.01274128814423138	0.08849122462022516
>10	0.00637064407211569	0.05407797060124871
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.002458089572784032	0.0
2	0.0	0.0	0.0	0.002458089572784032	0.0
3	0.0	0.0	0.0	0.002458089572784032	0.0
4	0.0	0.0	0.0	0.002458089572784032	0.0
5	0.0	0.0	0.0	0.007374268718352096	0.0
6	0.0	0.0	0.0	0.007374268718352096	0.0
7	0.0	0.0	0.0	0.007374268718352096	0.0
8	0.0	0.0	0.0	0.007374268718352096	0.0
9	0.0	0.0	0.0	0.007374268718352096	0.0
10	0.0	0.0	0.0	0.007374268718352096	0.0
11	0.0	0.0	0.0	0.007374268718352096	0.0
12	0.0	0.0	0.0	0.007374268718352096	0.0
13	0.0	0.0	0.0	0.007374268718352096	0.0
14	0.0	0.0	0.0	0.007374268718352096	0.0
15	0.0	0.0	0.0	0.009832358291136128	0.0
16	0.0	0.0	0.0	0.009832358291136128	0.0
17	0.0	0.0	0.0	0.009832358291136128	0.0
18	0.0	0.0	0.0	0.02212280615505629	0.0
19	0.0	0.0	0.0	0.02212280615505629	0.0
20	0.0	0.0	0.0	0.027038985300624353	0.0
21	0.0	0.0	0.0	0.03687134359176048	0.0
22	0.0	0.0	0.0	0.06391032889238484	0.0
23	0.0	0.0	0.0	0.06636841846516887	0.0
24	0.0	0.0	0.0	0.076200776756305	0.0
25	0.0	0.0	0.0	0.076200776756305	0.0
26	0.0	0.0	0.0	0.076200776756305	0.0
27	0.0	0.0	0.0	0.076200776756305	0.0
28	0.0	0.0	0.0	0.08111695590187307	0.0
29	0.0	0.0	0.0	0.0909493141930092	0.0
30	0.0	0.0	0.0	0.10569785162971339	0.0
31	0.0	0.0	0.0	0.12044638906641758	0.0
32	0.0	0.0	0.0	0.14011110564868984	0.0
33	0.0	0.0	0.0	0.16223391180374613	0.0
34	0.0	0.0	0.0	0.20156334496829065	0.0
35	0.0	0.0	0.0	0.2433508677056192	0.0
36	0.0	0.0	0.0	0.31217737574357207	0.0
37	0.0	0.0	0.0	0.5161988102846468	0.0
38	0.0	0.0	0.0	0.759549677990266	0.0
39	0.0	0.0	0.0	1.002900545695885	0.0
40	0.0	0.0	0.0	1.3347426380217295	0.0
41	0.0	0.0	0.0	1.6518361929108696	0.0
42	0.0	0.0	0.0	2.0525047932746667	0.0
43	0.0	0.0	0.0	2.509709453812497	0.0
44	0.0	0.0	0.0	2.890713337594022	0.0
45	0.0	0.0	0.0	3.404454058305885	0.0
46	0.0	0.0	0.0	3.9034462415810434	0.0
47	0.0	0.0	0.0	4.331153827245465	0.0
48	0.0	0.0	0.0	4.719531979745342	0.0
49	0.0	0.0	0.0	5.100535863526867	0.0
50	0.0	0.0	0.0	5.493830195172312	0.0
51	0.0	0.0	0.0	5.960867214001278	0.0
52	0.0	0.0	0.0	6.42544614325746	0.0
53	0.0	0.0	0.0	6.976058207561084	0.0
54	0.0	0.0	0.0	7.423430509807777	0.0
55	0.0	0.0	0.0	7.976500663684185	0.0
56	0.0	0.0	0.0	8.39437589105747	0.0
57	0.0	0.0	0.0	8.858954820313652	0.0
58	0.0	0.0	0.0	9.276830047686937	0.0
59	0.0	0.0	0.0	9.692247185487439	0.0
60	0.0	0.0	0.0	10.183865100044246	0.0
61	0.0	0.0	0.0	10.562410894252986	0.0
62	0.0	0.0	0.0	10.960621405044	0.0
63	0.0	0.0	0.0	11.439948871736886	0.0
64	0.0	0.0	0.0	11.855366009537388	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGAAC	15	0.002209455	70.00001	28
CTCGGTG	30	9.996256E-8	70.00001	44
AGTTGTA	15	0.002209455	70.00001	26
TAGTGTA	15	0.002209455	70.00001	34
TCGGTGG	30	9.996256E-8	70.00001	45
TCTCGGT	35	3.594323E-9	70.0	43
CGACGAA	35	2.904635E-7	60.0	22
GATCTCG	35	2.904635E-7	60.0	41
CGTATCA	25	2.36361E-4	55.999996	56
TGGTCGC	25	2.36361E-4	55.999996	49
GTGTAGA	70	0.0	55.0	36
GGTGGTC	45	2.6178895E-8	54.444447	47
CGGTGGT	45	2.6178895E-8	54.444447	46
ATCTCGG	40	7.30568E-7	52.499996	42
GTCGCCG	20	0.006904135	52.499996	51
GGTCGAC	20	0.006904135	52.499996	50
ATGCTTC	20	0.006904135	52.499996	6
CTAGTGT	20	0.006904135	52.499996	33
ACGTGTA	20	0.006904135	52.499996	34
CAGGCAT	20	0.006904135	52.499996	27
>>END_MODULE