FastQCFastQC Report
Wed 25 May 2016
SRR1779787_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779787_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40682
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT488312.00285138390443No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG7821.9222260459171132No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC3330.8185438277370828No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT3060.752175409271914Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC1740.42770758566442163No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT1120.27530603215181165No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT1050.2580994051423234No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC830.20402143454107466No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC810.1991052553955066No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT770.18927289710437048No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT440.10815594120249741No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCCG150.00220945570.0000143
CTATGGG150.00220945570.0000148
CCTCGTA309.996256E-870.0000138
TCTCGTC150.00220945570.0000138
GCTTAGT150.00220945570.0000118
TAGGGAG150.00220945570.000014
GCTTTCA150.00220945570.0000141
ACCTCGT308.236193E-658.33333637
ACAGACC308.236193E-658.33333633
TGATACC200.00690413552.49999610
GGAGCAA200.00690413552.49999622
GTACAAA200.00690413552.4999961
ATGGAGC200.00690413552.4999966
CCAATCA200.00690413552.49999651
CGTCTGC200.00690413552.49999641
ATCGTAT200.00690413552.49999639
ATATCGT200.00690413552.49999637
GATATCG200.00690413552.49999636
GAGCAAG200.00690413552.49999623
CCAACTA200.00690413552.4999966
Produced by FastQC (version 0.11.5)