##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779787_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40682
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.340396244039134	34.0	34.0	34.0	33.0	34.0
2	33.49488717368861	34.0	34.0	34.0	34.0	34.0
3	33.66850204021434	34.0	34.0	34.0	34.0	34.0
4	33.40135686544418	34.0	34.0	34.0	34.0	34.0
5	33.432795831080085	34.0	34.0	34.0	34.0	34.0
6	37.02052504793274	38.0	37.0	38.0	36.0	38.0
7	37.144855218524164	38.0	37.0	38.0	36.0	38.0
8	36.982277174180226	38.0	37.0	38.0	35.0	38.0
9	36.818420923258444	38.0	37.0	38.0	35.0	38.0
10-11	36.80309473477214	38.0	37.0	38.0	35.0	38.0
12-13	37.0013642397129	38.0	37.0	38.0	35.5	38.0
14-15	37.0474534192026	38.0	37.0	38.0	36.0	38.0
16-17	36.916744506169806	38.0	37.0	38.0	35.0	38.0
18-19	36.751855857627454	38.0	37.0	38.0	34.0	38.0
20-21	36.811906985890566	38.0	37.0	38.0	34.5	38.0
22-23	36.75001229044786	38.0	37.0	38.0	34.0	38.0
24-25	36.94342706848238	38.0	37.0	38.0	35.0	38.0
26-27	36.78989479376628	38.0	37.0	38.0	34.5	38.0
28-29	36.14646526719434	38.0	37.0	38.0	34.0	38.0
30-31	36.65821493535225	38.0	37.0	38.0	34.0	38.0
32-33	36.71818003048031	38.0	37.0	38.0	34.5	38.0
34-35	36.758701637087654	38.0	37.0	38.0	34.5	38.0
36-37	36.680399193746624	38.0	37.0	38.0	34.0	38.0
38-39	35.55357406223883	38.0	36.5	38.0	29.0	38.0
40-41	36.195934319846614	38.0	37.0	38.0	33.0	38.0
42-43	36.77364682169018	38.0	37.0	38.0	34.5	38.0
44-45	36.47541910427216	38.0	37.0	38.0	34.0	38.0
46-47	36.68798240007866	38.0	37.0	38.0	34.0	38.0
48-49	36.20287842288973	38.0	37.0	38.0	33.0	38.0
50-51	36.36690674008161	38.0	37.0	38.0	34.0	38.0
52-53	36.3150656309916	38.0	37.0	38.0	34.0	38.0
54-55	36.2662848434197	38.0	37.0	38.0	34.0	38.0
56-57	36.146600462120844	38.0	37.0	38.0	34.0	38.0
58-59	36.27174180227127	38.0	37.0	38.0	34.0	38.0
60-61	35.784204316405294	38.0	37.0	38.0	31.5	38.0
62-63	35.57520525047933	38.0	36.0	38.0	31.0	38.0
64-65	36.05944889631778	38.0	37.0	38.0	34.0	38.0
66-67	35.77169264047982	38.0	36.5	38.0	32.5	38.0
68-69	35.79849810727103	38.0	37.0	38.0	31.0	38.0
70-71	34.707216950985696	38.0	35.0	38.0	26.5	38.0
72-73	32.83450911951232	38.0	32.5	38.0	16.0	38.0
74-75	32.52675630499975	38.0	32.0	38.0	11.0	38.0
76	32.254338528095964	37.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	0.0
23	4.0
24	19.0
25	29.0
26	41.0
27	131.0
28	218.0
29	343.0
30	523.0
31	909.0
32	1407.0
33	2167.0
34	3759.0
35	6178.0
36	7309.0
37	17643.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.79303051914517	18.636106534195708	13.058554459828345	36.51230848683078
2	15.623617324615308	17.314782950690724	44.49633744653655	22.565262278157416
3	15.874342461039282	19.863821837667764	42.10707438179047	22.154761319502484
4	12.032348458777838	28.86534585320289	34.64923061796372	24.45307507005555
5	11.980728577749373	31.974829162774693	44.058797502580994	11.98564475689494
6	24.625141340150435	45.06907231699523	17.31969912983629	12.986087213018042
7	21.355882208347673	27.990265965291776	20.31119413991446	30.342657686446096
8	18.74047490290546	46.096553758418956	17.327073398554642	17.835897940120937
9	19.259131802762894	16.803500319551645	18.03746128508923	45.89990659259623
10-11	20.790521606607346	30.077184012585416	29.14556806450027	19.986726316306967
12-13	20.4119758123986	19.30706454943218	31.06410697605821	29.21685266211101
14-15	19.164495354210707	19.66102944791308	21.870851973845927	39.30362322403028
16-17	27.691608082198517	29.715844845386165	20.23376431837176	22.358782754043556
18-19	27.733395604935847	23.935647214984517	21.55007128459761	26.780885895482033
20-21	19.895777002113956	21.680350031955165	20.870409517722827	37.55346344820805
22-23	19.058797502580994	30.892040705963325	21.213313013126196	28.83584877832948
24-25	27.35362076594071	30.544221031414388	20.870409517722827	21.231748684922078
26-27	28.046802025465805	31.16120151418318	21.911410451796865	18.88058600855415
28-29	26.50557986333022	24.252740769873654	22.31822427609262	26.923455090703506
30-31	20.38370778231159	22.13509660292021	38.13357258738508	19.34762302738312
32-33	18.72572636546876	38.07826557199744	24.49363354800649	18.70237451452731
34-35	18.795781918293102	32.58812251118431	21.766383167002605	26.84971240351998
36-37	26.801779656850695	31.54957966668305	22.10314143847402	19.545499237992235
38-39	19.674548940563394	24.668157907674157	29.64456024777543	26.012732903987022
40-41	18.311538272454648	21.315323730396734	31.808908116611768	28.564229880536846
42-43	27.16311882404995	20.719237008996608	31.107123543581928	21.010520623371516
44-45	19.27633842977238	29.23774642347967	31.84209232584435	19.643822820903594
46-47	26.46379234059289	21.937220392311097	25.161004867017354	26.437982400078656
48-49	25.887370335775035	20.576667813775135	23.410845091195124	30.125116759254706
50-51	19.556506133687343	21.030311969909285	31.879194630872483	27.53398726553089
52-53	17.176051143348907	21.0142611261372	40.25694615195476	21.552741578559136
54-55	17.194570135746606	21.022280149518004	33.66614204210112	28.11700767263427
56-57	25.865037011533826	21.047389518727098	25.14202100189361	27.945552467845463
58-59	17.606343742316202	21.68797639537743	38.856651094172605	21.84902876813376
60-61	25.41768603779152	29.40583469590981	25.600865492187214	19.575613774111456
62-63	17.47286383360582	39.89108655300004	22.425598347859225	20.210451265534918
64-65	16.50727701838922	41.507277018389225	21.603648343003247	20.38179762021831
66-67	16.96498819826908	41.45849724626279	21.23205153422502	20.344463021243115
68-69	16.632660586543423	41.51773642419922	21.325499643550728	20.524103345706628
70-71	16.777286135693213	39.471484759095375	21.739183874139627	22.01204523107178
72-73	17.478365621005015	35.55290589045137	22.35470547743141	24.614023011112206
74-75	18.357363226274323	33.89136767579926	22.692577159924777	25.058691938001648
76	18.39724680432645	34.614060963618485	21.796951819075712	25.191740412979353
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	10.0
16	16.0
17	14.0
18	17.0
19	33.0
20	57.5
21	69.0
22	79.0
23	123.0
24	174.0
25	191.0
26	222.0
27	304.5
28	427.5
29	499.0
30	522.5
31	610.5
32	788.0
33	901.0
34	901.0
35	997.0
36	1138.5
37	1184.0
38	1375.0
39	2270.0
40	3496.5
41	3884.0
42	3749.0
43	3336.0
44	2516.5
45	1975.0
46	1840.0
47	1696.0
48	1511.0
49	1375.0
50	1280.0
51	1160.5
52	1042.5
53	990.5
54	937.0
55	913.0
56	868.0
57	745.0
58	643.0
59	633.0
60	574.5
61	498.5
62	471.0
63	468.5
64	409.0
65	371.5
66	348.5
67	306.0
68	294.5
69	272.0
70	238.5
71	216.0
72	209.0
73	173.0
74	137.0
75	130.0
76	116.0
77	87.5
78	71.0
79	69.0
80	62.0
81	41.0
82	27.0
83	27.0
84	24.5
85	18.0
86	10.5
87	7.0
88	6.5
89	5.0
90	4.0
91	2.5
92	1.0
93	1.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04670370188289661
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.01229044786392016
52-53	0.02949707487340839
54-55	0.04424561231011258
56-57	0.04670370188289661
58-59	0.02949707487340839
60-61	0.02826803008701637
62-63	0.018435671795880244
64-65	0.014748537436704194
66-67	0.02458089572784032
68-69	0.007374268718352097
70-71	0.004916179145568064
72-73	0.014748537436704194
74-75	0.008603313504744114
76	0.004916179145568064
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	40682.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.75689494125166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.799648506151144	24.974190059485768
2	22.35830404217926	20.01376530160759
3	11.895869947275923	15.972666043950642
4	5.574472759226714	9.979843665503171
5	2.570298769771529	5.751929600314636
6	0.9830843585237259	2.6399882011700506
7	0.3734622144112478	1.1700506366451993
8	0.12082601054481545	0.43262376480998965
9	0.032952548330404216	0.13273683693033775
>10	0.2361599297012302	1.8828966127525688
>50	0.016476274165202108	0.5923995870409517
>100	0.027460456942003518	2.5318322599675533
>500	0.005492091388400702	1.9222260459171132
>1k	0.005492091388400702	12.00285138390443
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT	4883	12.00285138390443	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	782	1.9222260459171132	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC	333	0.8185438277370828	No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT	306	0.752175409271914	Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC	174	0.42770758566442163	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT	112	0.27530603215181165	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT	105	0.2580994051423234	No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC	83	0.20402143454107466	No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC	81	0.1991052553955066	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT	77	0.18927289710437048	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT	44	0.10815594120249741	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007374268718352096	0.0
2	0.0	0.0	0.0	0.007374268718352096	0.0
3	0.0	0.0	0.0	0.007374268718352096	0.0
4	0.0	0.0	0.0	0.007374268718352096	0.0
5	0.0	0.0	0.0	0.01229044786392016	0.0
6	0.0	0.0	0.0	0.01229044786392016	0.0
7	0.0	0.0	0.0	0.01229044786392016	0.0
8	0.0	0.0	0.0	0.01229044786392016	0.0
9	0.0	0.0	0.0	0.01229044786392016	0.0
10	0.0	0.0	0.0	0.01229044786392016	0.0
11	0.0	0.0	0.0	0.01229044786392016	0.0
12	0.0	0.0	0.0	0.01229044786392016	0.0
13	0.0	0.0	0.0	0.01229044786392016	0.0
14	0.0	0.0	0.0	0.01229044786392016	0.0
15	0.0	0.0	0.0	0.014748537436704193	0.0
16	0.0	0.0	0.0	0.014748537436704193	0.0
17	0.0	0.0	0.0	0.014748537436704193	0.0
18	0.0	0.0	0.0	0.014748537436704193	0.0
19	0.0	0.0	0.0	0.014748537436704193	0.0
20	0.0	0.0	0.0	0.017206627009488225	0.0
21	0.0	0.0	0.0	0.03195516444619242	0.0
22	0.0	0.0	0.0	0.03195516444619242	0.0
23	0.0	0.0	0.0	0.03687134359176048	0.0
24	0.0	0.0	0.0	0.051619881028464674	0.0
25	0.0	0.0	0.0	0.051619881028464674	0.0
26	0.0	0.0	0.0	0.051619881028464674	0.0
27	0.0	0.0	0.0	0.051619881028464674	0.0
28	0.0	0.0	0.0	0.05653606017403274	0.0
29	0.0	0.0	0.0	0.06636841846516887	0.0
30	0.0	0.0	0.0	0.08357504547465709	0.0
31	0.0	0.0	0.0	0.10078167248414532	0.0
32	0.0	0.0	0.0	0.11798829949363354	0.0
33	0.0	0.0	0.0	0.14256919522147388	0.0
34	0.0	0.0	0.0	0.17944053881323435	0.0
35	0.0	0.0	0.0	0.22614424069613095	0.0
36	0.0	0.0	0.0	0.3097192861707881	0.0
37	0.0	0.0	0.0	0.533405437294135	0.0
38	0.0	0.0	0.0	0.7841305737181062	0.0
39	0.0	0.0	0.0	1.0127329039870212	0.0
40	0.0	0.0	0.0	1.3347426380217295	0.0
41	0.0	0.0	0.0	1.6493781033380857	0.0
42	0.0	0.0	0.0	2.042672434983531	0.0
43	0.0	0.0	0.0	2.494960916375793	0.0
44	0.0	0.0	0.0	2.8735067105845338	0.0
45	0.0	0.0	0.0	3.370040804286908	0.0
46	0.0	0.0	0.0	3.8616587188437146	0.0
47	0.0	0.0	0.0	4.26724349835308	0.0
48	0.0	0.0	0.0	4.665454009144093	0.0
49	0.0	0.0	0.0	5.039083624207266	0.0
50	0.0	0.0	0.0	5.427461776707143	0.0
51	0.0	0.0	0.0	5.879750258099405	0.0
52	0.0	0.0	0.0	6.327122560346099	0.0
53	0.0	0.0	0.0	6.877734624649722	0.0
54	0.0	0.0	0.0	7.3103583894597115	0.0
55	0.0	0.0	0.0	7.836389558035495	0.0
56	0.0	0.0	0.0	8.232141979253724	0.0
57	0.0	0.0	0.0	8.677056191927633	0.0
58	0.0	0.0	0.0	9.06543434442751	0.0
59	0.0	0.0	0.0	9.490683840519148	0.0
60	0.0	0.0	0.0	9.965095128066467	0.0
61	0.0	0.0	0.0	10.33872474312964	0.0
62	0.0	0.0	0.0	10.727102895629518	0.0
63	0.0	0.0	0.0	11.186765645740131	0.0
64	0.0	0.0	0.0	11.62430558969569	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGCCG	15	0.002209455	70.00001	43
CTATGGG	15	0.002209455	70.00001	48
CCTCGTA	30	9.996256E-8	70.00001	38
TCTCGTC	15	0.002209455	70.00001	38
GCTTAGT	15	0.002209455	70.00001	18
TAGGGAG	15	0.002209455	70.00001	4
GCTTTCA	15	0.002209455	70.00001	41
ACCTCGT	30	8.236193E-6	58.333336	37
ACAGACC	30	8.236193E-6	58.333336	33
TGATACC	20	0.006904135	52.499996	10
GGAGCAA	20	0.006904135	52.499996	22
GTACAAA	20	0.006904135	52.499996	1
ATGGAGC	20	0.006904135	52.499996	6
CCAATCA	20	0.006904135	52.499996	51
CGTCTGC	20	0.006904135	52.499996	41
ATCGTAT	20	0.006904135	52.499996	39
ATATCGT	20	0.006904135	52.499996	37
GATATCG	20	0.006904135	52.499996	36
GAGCAAG	20	0.006904135	52.499996	23
CCAACTA	20	0.006904135	52.499996	6
>>END_MODULE