FastQCFastQC Report
Wed 25 May 2016
SRR1779786_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779786_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18029
Sequences flagged as poor quality0
Sequence length76
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCT207.718856E-570.0000113
ACTAACG150.002189398370.011
GAGTGTA150.002189398370.034
GGGTAGA150.002189398370.036
GGATACC150.002189398370.038
GTCCATA150.002189398370.022
GACGATC150.002189398370.023
GTGGTAG150.002189398370.048
GCTTCCG150.002189398370.017
ATTTATC150.002189398370.045
CGACGAT308.0512455E-658.33333222
CTAACGC252.328154E-455.99999612
GATCTCT252.328154E-455.99999641
GTGGTCG252.328154E-455.99999648
GAGATGA252.328154E-455.9999964
GGTCGCC252.328154E-455.99999650
TGGTCGC200.00684193652.50000449
ATCTCTG200.00684193652.50000442
ATCTCGG200.00684193652.50000442
TGACTCT200.00684193652.50000413