##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779786_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18029
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.137888956680904	23.0	12.0	32.0	12.0	34.0
2	20.5729657773587	21.0	12.0	32.0	12.0	34.0
3	20.97359809196295	21.0	11.0	32.0	11.0	34.0
4	21.135226579399855	21.0	12.0	32.0	12.0	34.0
5	20.69887403627489	21.0	11.0	32.0	11.0	34.0
6	21.893560374951466	21.0	11.0	34.0	11.0	38.0
7	22.846469576792945	22.0	12.0	34.0	11.0	38.0
8	22.003605302568086	21.0	11.0	34.0	11.0	38.0
9	23.21853680181929	23.0	11.0	34.0	11.0	38.0
10-11	22.524183260302845	22.0	11.5	34.0	11.0	38.0
12-13	22.609129735426258	22.5	11.0	34.0	11.0	38.0
14-15	21.977647124077876	21.5	11.0	32.0	11.0	37.5
16-17	21.868822452715072	21.0	11.0	32.5	11.0	38.0
18-19	21.63026235509457	21.0	11.0	33.0	10.0	38.0
20-21	22.125270397692606	22.5	11.0	32.5	10.5	38.0
22-23	22.001053857673746	21.0	11.0	32.5	10.0	38.0
24-25	22.17141827056409	21.5	11.0	34.0	10.0	38.0
26-27	21.509096455710242	21.0	11.0	31.0	10.0	38.0
28-29	21.367574463364576	21.0	11.0	32.0	10.5	38.0
30-31	21.47448555105663	21.0	11.0	33.5	10.5	38.0
32-33	21.66867269399301	21.0	11.0	34.0	11.0	38.0
34-35	21.905818403682954	21.0	11.0	34.0	11.0	38.0
36-37	21.641993454989183	21.0	11.0	34.0	11.0	38.0
38-39	21.557213378445837	21.0	11.0	34.0	11.0	38.0
40-41	21.50435409617838	21.0	11.0	33.0	11.0	38.0
42-43	21.73240335015808	21.0	11.0	34.0	11.0	38.0
44-45	21.702035609296132	21.0	11.0	34.0	11.0	38.0
46-47	21.655028010427642	21.0	11.0	34.0	11.0	38.0
48-49	21.443646347551166	21.0	11.0	33.5	11.0	38.0
50-51	21.20464252038383	21.0	11.0	32.0	11.0	38.0
52-53	21.560319485273723	21.0	11.0	33.5	11.0	38.0
54-55	21.476953796660936	21.0	11.0	33.0	11.0	38.0
56-57	21.501774918187365	21.0	11.0	33.5	11.0	38.0
58-59	21.303483276942703	21.0	11.0	32.5	10.5	38.0
60-61	21.5912696211659	21.0	11.0	33.5	11.0	38.0
62-63	21.335265405735207	21.0	11.0	33.0	11.0	38.0
64-65	21.29516334793943	21.0	11.0	32.5	10.5	38.0
66-67	21.71293471629042	21.0	11.0	33.0	11.0	38.0
68-69	21.554856065228243	21.0	11.0	32.0	10.0	38.0
70-71	21.565034111708915	21.0	11.0	32.0	10.0	38.0
72-73	21.007293804426205	21.0	11.0	31.0	10.0	38.0
74-75	21.24108380941816	21.0	11.0	31.5	10.0	38.0
76	20.843197071384992	21.0	11.0	31.0	10.0	37.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
11	2.0
12	19.0
13	289.0
14	969.0
15	1686.0
16	2093.0
17	2180.0
18	2054.0
19	1724.0
20	1157.0
21	854.0
22	529.0
23	375.0
24	252.0
25	195.0
26	163.0
27	108.0
28	87.0
29	97.0
30	106.0
31	111.0
32	136.0
33	169.0
34	210.0
35	344.0
36	659.0
37	1461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.71984025736314	20.716623218148538	8.774751788784735	51.78878473570359
2	24.97642686782406	21.02723390093738	27.261634034056243	26.73470519718232
3	9.456985967053082	33.88429752066116	32.31460424871041	24.34411226357535
4	21.909146375284262	35.8200676687559	15.79677186754673	26.47401408841311
5	13.761162571412724	30.600698874036276	34.01741638471351	21.620722169837485
6	27.572244716845084	40.21853680181929	11.104331909701038	21.104886571634587
7	15.824504964224307	39.525209384879915	11.154251483720671	33.49603416717511
8	13.306339785900493	43.324643629707694	20.051028897886738	23.317987686505077
9	20.577957734760663	21.23800543568695	13.988573964168838	44.19546286538355
10-11	13.425592101614066	35.05740751012258	20.938487991569136	30.57851239669421
12-13	13.37844583726219	24.585390204670254	25.647567807421378	36.38859615064618
14-15	28.082533695712463	28.570636197237782	17.202839869099783	26.14399023794997
16-17	30.76155083476621	24.174940373842144	15.214376837317655	29.849131954073993
18-19	19.62671251871984	23.825503355704697	20.597370902434967	35.9504132231405
20-21	26.706972100504743	22.85484497198957	17.091907482389484	33.346275445116206
22-23	30.817017028121356	21.967385878307173	15.092351211936325	32.12324588163514
24-25	18.836319263408953	33.73176548893449	14.068999944533806	33.36291530312275
26-27	24.258139663874868	34.03960286205558	16.528925619834713	25.173331854234842
28-29	20.442065561040547	30.104276443507683	21.226912197015917	28.226745798435854
30-31	22.27799656109601	27.67763048421987	22.386155638138554	27.658217316545564
32-33	15.893837705918243	32.45326973209829	21.98125242664596	29.671640135337512
34-35	14.60702202007876	37.836263797215594	19.188529591214156	28.368184591491485
36-37	24.1679609496339	26.356223652096737	19.97448413578877	29.501331262480583
38-39	26.166902404526166	21.87369997503952	24.91333166930138	27.04606595113293
40-41	18.736480115369684	30.44816684230961	20.888568417549504	29.9267846247712
42-43	12.160962893116647	29.399855787897277	24.061234677464086	34.377946641521994
44-45	12.194580802618077	23.792883490029677	30.321435504895028	33.69110020245722
46-47	16.329700196910444	28.884821255234765	24.577751892836343	30.20772665501844
48-49	18.076432414443396	30.55077930001664	20.941261301236896	30.43152698430307
50-51	26.701425481169228	28.936713073381775	15.305896056353651	29.055965389095345
52-53	11.163190592411803	33.89449744841358	20.837031284668296	34.105280674506325
54-55	15.145464126244557	26.19740965693208	21.23304767451535	37.42407854230802
56-57	19.05316582078376	29.752891255512104	18.112987769365173	33.080955154338966
58-59	16.978672657181683	29.888787198047535	18.085253903541616	35.047286241229166
60-61	12.72222992650118	26.800721120510328	21.028983497434474	39.448065455554016
62-63	16.904260039937874	27.75404925671178	19.306079431994675	36.03561127135567
64-65	17.417350787663636	26.54759263368094	18.6543155092079	37.380741069447524
66-67	13.717550477035722	37.67472820057688	13.817395163079654	34.790326159307746
68-69	15.397415275389651	40.77042542570303	12.147096344777857	31.685062954129457
70-71	16.015863331299574	37.98324920960675	12.036163958067558	33.96472350102612
72-73	18.434189361584114	36.71862000110933	13.214820566864496	31.632370070442068
74-75	14.681901381108215	34.938155194409006	15.54162737811304	34.83831604636974
76	12.208109157468524	34.095069055410725	16.467912807144046	37.22890897997671
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.5
17	3.0
18	2.5
19	2.0
20	3.0
21	4.0
22	6.5
23	15.5
24	34.5
25	47.0
26	34.5
27	32.0
28	52.5
29	63.0
30	71.5
31	88.0
32	114.5
33	133.0
34	142.0
35	186.0
36	246.0
37	271.0
38	322.0
39	413.0
40	493.5
41	584.0
42	634.0
43	689.5
44	812.5
45	930.5
46	981.0
47	1042.5
48	1093.0
49	1169.5
50	1257.0
51	1197.0
52	1096.5
53	1006.5
54	957.0
55	952.5
56	882.0
57	745.0
58	674.0
59	644.0
60	590.0
61	483.5
62	401.0
63	378.0
64	333.5
65	269.0
66	208.5
67	191.0
68	164.0
69	119.5
70	87.0
71	72.0
72	63.0
73	47.0
74	38.5
75	37.0
76	30.5
77	22.0
78	19.0
79	18.0
80	17.5
81	12.5
82	7.0
83	6.0
84	6.0
85	5.0
86	3.5
87	3.0
88	2.5
89	2.0
90	1.5
91	1.5
92	2.0
93	3.0
94	2.5
95	1.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.005546619335515004
38-39	0.002773309667757502
40-41	0.0
42-43	0.0
44-45	0.002773309667757502
46-47	0.002773309667757502
48-49	0.0
50-51	0.0
52-53	0.005546619335515004
54-55	0.002773309667757502
56-57	0.002773309667757502
58-59	0.002773309667757502
60-61	0.008319929003272506
62-63	0.005546619335515004
64-65	0.005546619335515004
66-67	0.005546619335515004
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	18029.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.51278495756836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.04994192799072	92.69510233512675
2	1.8292682926829267	3.4943701813744523
3	0.7317073170731708	2.096622108824671
4	0.2380952380952381	0.9096455710244606
5	0.10452961672473868	0.4991957401963503
6	0.029036004645760744	0.1663985800654501
7	0.005807200929152149	0.03882633534860502
8	0.0	0.0
9	0.011614401858304297	0.09983914803927006
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005546619335515004	0.0
2	0.0	0.0	0.0	0.005546619335515004	0.0
3	0.0	0.0	0.0	0.005546619335515004	0.0
4	0.0	0.0	0.0	0.005546619335515004	0.0
5	0.0	0.0	0.0	0.011093238671030008	0.0
6	0.0	0.0	0.0	0.011093238671030008	0.0
7	0.0	0.0	0.0	0.011093238671030008	0.0
8	0.0	0.0	0.0	0.011093238671030008	0.0
9	0.0	0.0	0.0	0.011093238671030008	0.0
10	0.0	0.0	0.0	0.011093238671030008	0.0
11	0.0	0.0	0.0	0.011093238671030008	0.0
12	0.0	0.0	0.0	0.011093238671030008	0.0
13	0.0	0.0	0.0	0.011093238671030008	0.0
14	0.0	0.0	0.0	0.011093238671030008	0.0
15	0.0	0.0	0.0	0.011093238671030008	0.0
16	0.0	0.0	0.0	0.011093238671030008	0.0
17	0.0	0.0	0.0	0.011093238671030008	0.0
18	0.0	0.0	0.0	0.011093238671030008	0.0
19	0.0	0.0	0.0	0.022186477342060015	0.0
20	0.0	0.0	0.0	0.02773309667757502	0.0
21	0.0	0.0	0.0	0.02773309667757502	0.0
22	0.0	0.0	0.0	0.09429252870375505	0.0
23	0.0	0.0	0.0	0.09429252870375505	0.0
24	0.0	0.0	0.0	0.1331188640523601	0.0
25	0.0	0.0	0.0	0.1331188640523601	0.0
26	0.0	0.0	0.0	0.1386654833878751	0.0
27	0.0	0.0	0.0	0.1386654833878751	0.0
28	0.0	0.0	0.0	0.17194519940096512	0.0
29	0.0	0.0	0.0	0.17749181873648012	0.005546619335515004
30	0.0	0.0	0.0	0.19967829607854012	0.005546619335515004
31	0.0	0.0	0.0	0.20522491541405513	0.005546619335515004
32	0.0	0.0	0.0	0.21077153474957014	0.005546619335515004
33	0.0	0.0	0.0	0.22741139275611516	0.005546619335515004
34	0.0	0.0	0.0	0.24405125076266015	0.005546619335515004
35	0.0	0.0	0.0	0.24959787009817516	0.005546619335515004
36	0.0	0.0	0.0	0.3050640634533252	0.005546619335515004
37	0.0	0.0	0.0	0.4770092628542903	0.005546619335515004
38	0.0	0.0	0.0	0.6378612235842254	0.005546619335515004
39	0.0	0.0	0.0	0.8153530423207055	0.005546619335515004
40	0.0	0.0	0.0	0.9484719063730657	0.005546619335515004
41	0.0	0.0	0.0	1.1204171057740306	0.005546619335515004
42	0.0	0.0	0.0	1.3034555438460258	0.005546619335515004
43	0.0	0.0	0.0	1.442121027233901	0.005546619335515004
44	0.0	0.0	0.0	1.6029729879638361	0.005546619335515004
45	0.0	0.0	0.0	1.7804648067003161	0.005546619335515004
46	0.0	0.0	0.0	1.9856897221143712	0.005546619335515004
47	0.0	0.0	0.0	2.1853680181929116	0.005546619335515004
48	0.0	0.0	0.0	2.3406733595873317	0.005546619335515004
49	0.0	0.0	0.0	2.5680847523434465	0.005546619335515004
50	0.0	0.0	0.0	2.806589383770592	0.005546619335515004
51	0.0	0.0	0.0	3.006267679849132	0.005546619335515004
52	0.0	0.0	0.0	3.133839924565977	0.005546619335515004
53	0.0	0.0	0.0	3.3057851239669422	0.005546619335515004
54	0.0	0.0	0.0	3.4444506073548173	0.005546619335515004
55	0.0	0.0	0.0	3.5110100393809973	0.005546619335515004
56	0.0	0.0	0.0	3.6718620001109326	0.005546619335515004
57	0.0	0.0	0.0	3.7495146708081424	0.005546619335515004
58	0.0	0.0	0.0	3.9214598702091075	0.005546619335515004
59	0.0	0.0	0.0	4.137778024294192	0.005546619335515004
60	0.0	0.0	0.0	4.259803649675523	0.005546619335515004
61	0.0	0.0	0.0	4.459481945754063	0.005546619335515004
62	0.0	0.0	0.0	4.575960951799878	0.005546619335515004
63	0.0	0.0	0.0	4.747906151200843	0.005546619335515004
64	0.0	0.0	0.0	4.892118253924234	0.005546619335515004
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCT	20	7.718856E-5	70.00001	13
ACTAACG	15	0.0021893983	70.0	11
GAGTGTA	15	0.0021893983	70.0	34
GGGTAGA	15	0.0021893983	70.0	36
GGATACC	15	0.0021893983	70.0	38
GTCCATA	15	0.0021893983	70.0	22
GACGATC	15	0.0021893983	70.0	23
GTGGTAG	15	0.0021893983	70.0	48
GCTTCCG	15	0.0021893983	70.0	17
ATTTATC	15	0.0021893983	70.0	45
CGACGAT	30	8.0512455E-6	58.333332	22
CTAACGC	25	2.328154E-4	55.999996	12
GATCTCT	25	2.328154E-4	55.999996	41
GTGGTCG	25	2.328154E-4	55.999996	48
GAGATGA	25	2.328154E-4	55.999996	4
GGTCGCC	25	2.328154E-4	55.999996	50
TGGTCGC	20	0.006841936	52.500004	49
ATCTCTG	20	0.006841936	52.500004	42
ATCTCGG	20	0.006841936	52.500004	42
TGACTCT	20	0.006841936	52.500004	13
>>END_MODULE