SRR1779786_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1779786_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18029
Sequences flagged as poor quality	0
Sequence length	76
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT	8644	47.94497753619169	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	1251	6.938820788729269	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	577	3.2003993565921567	No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT	358	1.9856897221143712	RNA PCR Primer, Index 42 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	226	1.2535359698263908	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT	193	1.0704975317543957	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT	175	0.9706583837151256	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT	166	0.9207388096954906	No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	123	0.6822341782683454	RNA PCR Primer, Index 32 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	116	0.6434078429197404	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT	76	0.4215430694991403	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCG	69	0.38271673415053525	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	57	0.3161573021243552	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	56	0.3106106827888402	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCG	55	0.3050640634533252	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCCCTCGATCTCGTATGCCGT	46	0.25514448943369017	No Hit
CCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	45	0.24959787009817516	No Hit
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	45	0.24959787009817516	No Hit
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT	45	0.24959787009817516	RNA PCR Primer, Index 42 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	43	0.23850463142714515	RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTCTGCCGT	40	0.22186477342060013	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCAGT	38	0.21077153474957014	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACCCGATCTCGTATGCCGT	36	0.19967829607854012	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCG	31	0.17194519940096512	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCC	30	0.1663985800654501	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAACTCGATCTCGTATGCCGT	30	0.1663985800654501	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCCGT	28	0.1553053413944201	No Hit
TCTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT	28	0.1553053413944201	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCACTCGATCTCGTATGCCGT	26	0.14421210272339008	No Hit
CTTATACACATCTCCGAGCCCACGAGAAATCACTCGATCTCGTATGCCGT	23	0.12757224471684508	No Hit
CTTATACACCTCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT	21	0.11647900604581508	No Hit
TCCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	21	0.11647900604581508	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCC	20	0.11093238671030006	No Hit
CTTATACACATCTCCGAGACCACGAGACATCACTCGATCTCGTATGCCGT	20	0.11093238671030006	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTACTCGATCTCGTATGCCGT	19	0.10538576737478507	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTATA	20	7.718856E-5	70.00001	1
TTATGCT	15	0.0021893983	70.0	52
TATGCTT	15	0.0021893983	70.0	53
ATGCTTG	15	0.0021893983	70.0	54
GTCTTAT	15	0.0021893983	70.0	49
GCTTGAC	15	0.0021893983	70.0	56
CGTCTTA	15	0.0021893983	70.0	48
GTCTGCC	25	2.328154E-4	55.999996	42
TCTGCCG	25	2.328154E-4	55.999996	43
TCTCGTC	25	2.328154E-4	55.999996	38
TCGTCTG	25	2.328154E-4	55.999996	40
CTGCCGT	25	2.328154E-4	55.999996	44
CTCGTCT	25	2.328154E-4	55.999996	39
CGTCTGC	25	2.328154E-4	55.999996	41
CTTTATA	45	2.509114E-8	54.444447	2
TCTTTAT	45	2.509114E-8	54.444447	1
ACTCGAG	20	0.006841936	52.500004	32
GAAACAA	20	0.006841936	52.500004	60
GACATCC	20	0.006841936	52.500004	26
CTTATGC	20	0.006841936	52.500004	51

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content