FastQCFastQC Report
Wed 25 May 2016
SRR1779785_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779785_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164885
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTA150.002222508769.9921140
CGCTTAT308.354E-658.3267650
GCACCGT200.00694462552.4940836
ATCGTGA200.00694462552.49408329
TGCATCA200.00694462552.49408310
CTTGAAT200.00694462552.49408312
GTCGCTT352.0810146E-549.99436648
CTTCCGA352.0810146E-549.99436618
TAGATCT1150.048.69016639
TCGCCGT305.8591645E-446.67556852
GCCGAGT305.8591645E-446.67556858
CCGAGTT305.8591645E-446.67556859
GCATCGG305.8679364E-446.6614078
ATCTCGG851.8189894E-1245.28901742
GATCTCG851.8189894E-1245.28901741
GTCGCCG703.8016879E-1044.9949351
CGACGAA1100.044.54043622
GTAGATC1350.044.06910738
CGACGAC404.5806613E-543.74506822
GACGAAT404.5806613E-543.74506823
Produced by FastQC (version 0.11.5)