##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779785_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	164885
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.18500166782909	34.0	31.0	34.0	12.0	34.0
2	30.9511902234891	34.0	32.0	34.0	21.0	34.0
3	30.452933862995422	34.0	32.0	34.0	12.0	34.0
4	30.542735846195832	34.0	32.0	34.0	21.0	34.0
5	31.181884343633442	34.0	34.0	34.0	21.0	34.0
6	33.84618370379355	38.0	34.0	38.0	21.0	38.0
7	34.169627316008125	38.0	35.0	38.0	21.0	38.0
8	33.41711495891076	38.0	34.0	38.0	11.0	38.0
9	34.308536252539646	38.0	36.0	38.0	22.0	38.0
10-11	32.904487976468445	37.5	31.0	38.0	16.5	38.0
12-13	33.828277284167754	38.0	34.0	38.0	21.0	38.0
14-15	32.528792794978315	37.0	31.0	38.0	11.5	38.0
16-17	33.204936774115296	37.5	32.5	38.0	16.0	38.0
18-19	34.08085332201232	38.0	35.0	38.0	21.0	38.0
20-21	32.75122661248749	37.5	30.5	38.0	16.0	38.0
22-23	33.67528277284168	38.0	35.0	38.0	16.0	38.0
24-25	34.01898292749492	38.0	35.0	38.0	21.0	38.0
26-27	32.8736270734148	38.0	33.0	38.0	11.0	38.0
28-29	34.16405676683749	38.0	35.0	38.0	22.0	38.0
30-31	34.517824544379415	38.0	37.0	38.0	23.0	38.0
32-33	34.619798647542225	38.0	37.0	38.0	23.0	38.0
34-35	34.617127088576886	38.0	36.5	38.0	23.5	38.0
36-37	34.142893531855535	38.0	35.5	38.0	22.0	38.0
38-39	34.090396336840826	38.0	35.5	38.0	21.5	38.0
40-41	34.10411498923492	38.0	35.5	38.0	21.5	38.0
42-43	34.42870182248234	38.0	36.5	38.0	22.5	38.0
44-45	34.51258756102738	38.0	36.0	38.0	23.0	38.0
46-47	34.438071989568485	38.0	36.0	38.0	22.5	38.0
48-49	34.19783485459563	38.0	35.5	38.0	22.0	38.0
50-51	33.59386845377081	38.0	35.0	38.0	16.0	38.0
52-53	34.15069290717773	38.0	35.5	38.0	22.0	38.0
54-55	34.29203384176851	38.0	36.0	38.0	22.5	38.0
56-57	34.30345695484732	38.0	36.0	38.0	22.5	38.0
58-59	34.50023652848955	38.0	37.0	38.0	23.0	38.0
60-61	34.420335385268515	38.0	36.0	38.0	23.0	38.0
62-63	34.402898990205294	38.0	36.0	38.0	22.5	38.0
64-65	34.44526488158414	38.0	36.5	38.0	23.0	38.0
66-67	34.23350820268672	38.0	36.0	38.0	22.0	38.0
68-69	33.62608484701458	38.0	34.0	38.0	21.0	38.0
70-71	33.6411953785972	38.0	34.0	38.0	21.0	38.0
72-73	33.950386633107925	38.0	35.0	38.0	21.5	38.0
74-75	34.07738726991539	38.0	35.5	38.0	22.0	38.0
76	33.17794220214088	37.0	33.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	26.0
13	298.0
14	1155.0
15	2122.0
16	2554.0
17	2848.0
18	2759.0
19	2255.0
20	1797.0
21	1344.0
22	1154.0
23	971.0
24	926.0
25	1033.0
26	1134.0
27	1346.0
28	1711.0
29	1920.0
30	2575.0
31	3396.0
32	4457.0
33	6149.0
34	9081.0
35	14721.0
36	29293.0
37	67860.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	37.71840980077024	21.057706886617947	12.566940594960124	28.656942717651695
2	18.26485125996907	23.554598659671893	33.80174060708979	24.378809473269246
3	17.410316281044363	25.1939230372684	30.786912090244716	26.60884859144252
4	13.124298753676806	20.314764836097886	33.87209267064924	32.688843739576065
5	13.11156260423932	35.18209661279073	35.59935712769506	16.106983655274888
6	27.288606397302313	38.296478693853786	18.519911209228418	15.895003699615481
7	25.633047665835747	31.33790643934569	21.29223603049546	21.7368098643231
8	22.518998320020863	37.47566456214407	20.349581218197148	19.655755899637924
9	23.535833858993644	17.061720607501577	21.235867824736765	38.16657770876801
10-11	24.049393048947273	25.991697067287717	25.419771168991062	24.539138714773944
12-13	23.610395123874213	23.473936379901144	26.066652515389517	26.849015980835127
14-15	23.845552024453557	25.218032071031754	23.357936488682952	27.578479415831737
16-17	24.323390009370197	26.817863413480346	24.049865208676376	24.80888136847308
18-19	22.135122103584113	26.55680932554183	26.122555426505862	25.185513144368194
20-21	23.300785395881977	26.073627073414805	26.139430512175153	24.486157018528065
22-23	24.634587159301805	25.993437731225967	24.666731359396415	24.705243750075812
24-25	22.57149875212654	27.789823538866028	24.813728730808073	24.824948978199362
26-27	22.893904521043517	27.76792239416076	25.448419954574263	23.889753130221457
28-29	22.214034225824943	27.352760112933698	26.543361506106123	23.88984415513524
30-31	22.89262213057586	27.120417260514905	26.002971768202084	23.98398884070716
32-33	21.493465142371956	27.80210449707372	26.116384146526368	24.58804621402796
34-35	22.017291415852185	27.66201983873071	26.13029515494649	24.190393590470617
36-37	23.50935830904409	25.967298237526048	26.08376528250841	24.439578170921454
38-39	23.28025158987454	26.28713877171009	26.77994680705883	23.65266283135654
40-41	22.254265145588086	26.532753528259434	26.959722955065107	24.253258371087377
42-43	21.219592074380014	26.822352819878336	26.806887307969895	25.151167797771752
44-45	20.876901428952667	25.935248307819208	27.542516800504625	25.645333462723503
46-47	21.469553614750122	25.86335516739447	27.706210577389616	24.960880640465792
48-49	21.464050702004428	26.610364799708886	27.35209388361585	24.57349061467083
50-51	22.74502668607472	26.24393498301795	26.24423823386706	24.766800097040274
52-53	19.7991696261498	26.866445678446976	27.525467729755466	25.808916965647754
54-55	20.31187452236253	26.43594502468552	27.10615379016704	26.146026662784916
56-57	20.42993794772566	26.573901650481314	27.08554479227956	25.91061560951347
58-59	20.21253688612839	26.61260239407274	26.94317489104755	26.231685828751317
60-61	19.76590414141598	25.904020091357438	27.054177510054778	27.275898257171804
62-63	20.148237556142547	26.142942491227966	27.071246827055173	26.637573125574317
64-65	20.977809861205472	25.992126079782587	26.09464476366107	26.935419295350872
66-67	20.443661266988364	27.005468425378588	25.404065985059642	27.146804322573402
68-69	20.65233542267466	27.731836527993835	24.981123149841252	26.634704899490245
70-71	21.310913667101314	26.990326591260576	24.803954271158688	26.894805470479426
72-73	21.938342289450382	26.91050352381761	24.09266245345043	27.058491733281574
74-75	21.349939954146805	26.66672731904363	24.60363671652292	27.37969601028664
76	21.26927905580388	26.64224501307003	24.298130166605006	27.790345764521078
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.5
8	1.0
9	0.5
10	0.0
11	0.5
12	1.0
13	1.5
14	3.5
15	8.0
16	16.5
17	22.0
18	43.5
19	64.0
20	70.0
21	77.0
22	120.0
23	192.5
24	306.0
25	390.0
26	495.0
27	666.5
28	879.0
29	1025.0
30	1190.5
31	1570.5
32	2153.0
33	2521.0
34	2908.0
35	3735.5
36	4630.5
37	5085.0
38	5569.0
39	6817.5
40	7950.5
41	8720.5
42	9122.0
43	9416.0
44	10068.0
45	10338.0
46	10250.0
47	10398.5
48	10122.0
49	9453.5
50	9210.0
51	8711.5
52	7491.5
53	6471.5
54	6173.0
55	5708.0
56	4827.5
57	3934.5
58	3457.0
59	3051.0
60	2481.0
61	2175.5
62	2034.0
63	1788.0
64	1438.0
65	1286.0
66	1141.0
67	1044.0
68	962.5
69	843.5
70	749.0
71	692.0
72	673.5
73	574.5
74	465.0
75	436.0
76	421.0
77	381.0
78	322.0
79	288.0
80	242.0
81	187.5
82	148.5
83	118.0
84	110.0
85	95.5
86	73.0
87	57.0
88	58.5
89	47.5
90	32.0
91	25.0
92	21.0
93	18.0
94	12.0
95	6.0
96	3.0
97	4.0
98	6.0
99	8.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0018194499196409618
7	0.004851866452375898
8	0.0012129666130939745
9	0.007884282985110834
10-11	0.0021226915729144554
12-13	0.0
14-15	6.064833065469872E-4
16-17	3.032416532734936E-4
18-19	0.002729174879461443
20-21	0.0
22-23	0.0018194499196409618
24-25	0.002729174879461443
26-27	3.032416532734936E-4
28-29	0.005761591412196379
30-31	0.0
32-33	0.0
34-35	0.00333565818600843
36-37	0.0191042241562301
38-39	0.008187524638384328
40-41	0.0012129666130939745
42-43	0.0012129666130939745
44-45	0.005458349758922886
46-47	0.0030324165327349365
48-49	0.0
50-51	0.0030324165327349365
52-53	0.011826424477666252
54-55	0.00667131637201686
56-57	0.014555599357127694
58-59	0.011826424477666252
60-61	0.023046365648785518
62-63	0.008794007944931315
64-65	0.02243988234223853
66-67	0.017891257543136127
68-69	3.032416532734936E-4
70-71	0.0
72-73	0.004245383145828911
74-75	0.00667131637201686
76	0.002425933226187949
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	164885.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.700397246565785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.03137031807145	45.39103011189617
2	10.5214502676839	12.562695211814296
3	5.556853622113636	9.95239106043606
4	2.909475095746518	6.947872759802287
5	1.5746111726281784	4.700245625739152
6	0.9549254853357985	3.4205658489250084
7	0.5404471895730264	2.2585438335809807
8	0.4022877576521024	1.9213391151408559
9	0.27123947296240236	1.4573793856324104
>10	1.2302284710017575	11.150802074172908
>50	0.007111147231224032	0.23713497285987203
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0012129666130939747	0.0
2	0.0	0.0	0.0	0.0012129666130939747	0.0
3	0.0	0.0	0.0	0.0012129666130939747	0.0
4	0.0	0.0	0.0	0.0036388998392819236	0.0
5	0.0	0.0	0.0	0.004851866452375899	0.0
6	0.0	0.0	0.0	0.004851866452375899	0.0
7	0.0	0.0	0.0	0.004851866452375899	0.0
8	0.0	0.0	0.0	0.004851866452375899	0.0
9	0.0	0.0	0.0	0.00667131637201686	0.0
10	0.0	0.0	0.0	0.007277799678563847	0.0
11	0.0	0.0	0.0	0.007277799678563847	0.0
12	0.0	0.0	0.0	0.007277799678563847	0.0
13	0.0	0.0	0.0	0.007277799678563847	0.0
14	0.0	0.0	0.0	0.007277799678563847	0.0
15	0.0	0.0	0.0	0.010310216211298785	0.0
16	0.0	0.0	0.0	0.010916699517845772	0.0
17	0.0	0.0	0.0	0.013949116050580707	0.0
18	0.0	0.0	0.0	0.013949116050580707	0.0
19	0.0	0.0	0.0	0.015162082663674682	0.0
20	0.0	0.0	0.0	0.016981532583315643	0.0
21	0.0	0.0	0.0	0.019407465809503595	0.0
22	0.0	0.0	0.0	0.02486581556842648	0.0
23	0.0	0.0	0.0	0.025472298874973466	0.0
24	0.0	0.0	0.0	0.030930648633896354	0.0
25	0.0	0.0	0.0	0.032143615246990324	0.0
26	0.0	0.0	0.0	0.03275009855353731	0.0
27	0.0	0.0	0.0	0.0430603147648361	0.0
28	0.0	0.0	0.0	0.06004184734815174	0.0
29	0.0	0.0	0.0	0.08066227977074931	0.0
30	0.0	0.0	0.0	0.10613457864572277	0.0
31	0.0	0.0	0.0	0.13281984413379022	0.0
32	0.0	0.0	0.0	0.16617642599387453	0.0
33	0.0	0.0	0.0	0.19953300785395883	0.0
34	0.0	0.0	0.0	0.25593595536282865	0.0
35	0.0	0.0	0.0	0.35236680110379964	0.0
36	0.0	0.0	0.0	0.4815477453983079	0.0
37	0.0	0.0	0.0	0.7405161172938715	0.0
38	0.0	0.0	0.0	1.0807532522667314	0.0
39	0.0	0.0	0.0	1.4367589532098128	0.0
40	0.0	0.0	0.0	1.9625799799860508	0.0
41	0.0	0.0	0.0	2.496891773053947	0.0
42	0.0	0.0	0.0	3.0857870637110714	0.0
43	0.0	0.0	0.0	3.7535251842193045	0.0
44	0.0	0.0	0.0	4.423689237953726	0.0
45	0.0	0.0	0.0	5.1975619371076816	0.0
46	0.0	0.0	0.0	5.907753889074203	0.0
47	0.0	0.0	0.0	6.673135821936501	0.0
48	0.0	0.0	0.0	7.326924826394153	0.0
49	0.0	0.0	0.0	7.978894380932164	0.0
50	0.0	0.0	0.0	8.656336234345149	0.0
51	0.0	0.0	0.0	9.308912272189708	0.0
52	0.0	0.0	0.0	10.062771022227613	0.0
53	0.0	0.0	0.0	10.780847257179246	0.0
54	0.0	0.0	0.0	11.484974376080299	0.0
55	0.0	0.0	0.0	12.122388331261183	0.0
56	0.0	0.0	0.0	12.784668102010492	0.0
57	0.0	0.0	0.0	13.357188343390849	0.0
58	0.0	0.0	0.0	13.965491099857477	0.0
59	0.0	0.0	0.0	14.5646966067259	0.0
60	0.0	0.0	0.0	15.181490129484185	0.0
61	0.0	0.0	0.0	15.75643630409073	0.0
62	0.0	0.0	0.0	16.306516663128846	0.0
63	0.0	0.0	0.0	16.900870303544895	0.0
64	0.0	0.0	0.0	17.558904691148378	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGGTA	15	0.0022225087	69.99211	40
CGCTTAT	30	8.354E-6	58.32676	50
GCACCGT	20	0.006944625	52.494083	6
ATCGTGA	20	0.006944625	52.494083	29
TGCATCA	20	0.006944625	52.494083	10
CTTGAAT	20	0.006944625	52.494083	12
GTCGCTT	35	2.0810146E-5	49.994366	48
CTTCCGA	35	2.0810146E-5	49.994366	18
TAGATCT	115	0.0	48.690166	39
TCGCCGT	30	5.8591645E-4	46.675568	52
GCCGAGT	30	5.8591645E-4	46.675568	58
CCGAGTT	30	5.8591645E-4	46.675568	59
GCATCGG	30	5.8679364E-4	46.661407	8
ATCTCGG	85	1.8189894E-12	45.289017	42
GATCTCG	85	1.8189894E-12	45.289017	41
GTCGCCG	70	3.8016879E-10	44.99493	51
CGACGAA	110	0.0	44.540436	22
GTAGATC	135	0.0	44.069107	38
CGACGAC	40	4.5806613E-5	43.745068	22
GACGAAT	40	4.5806613E-5	43.745068	23
>>END_MODULE