FastQCFastQC Report
Wed 25 May 2016
SRR1779785_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779785_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164885
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT111666.77199260090366No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG15260.9254935257907027No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC8070.48943202838341876No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT4400.2668526548806744RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC2860.17345422567243837No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA150.00221758470.0312649
CGTCAAT150.002222929469.9887832
GACATTA200.006929279352.52344555
TCCGCGC200.006945933752.49158513
GTCAATC200.006945933752.49158533
GATGCGT200.006945933752.4915855
TTTGACC407.453182E-752.49158538
AGGATGC200.006945933752.4915853
CTCGTAT19600.051.77741239
TCGTATG19950.051.7460940
TATGCCG19750.051.73853743
CGTATGC19900.051.70025341
ATCTCGT18750.051.69837637
TCTCGTA18750.051.69837638
GCCGTCT19800.051.62354346
ATGCCGT19850.051.4778944
GTATGCC19950.051.21985642
CGTCTTC19900.051.18822548
CCGTCTT19900.051.18822547
TGCCGTC19900.051.18822545
Produced by FastQC (version 0.11.5)