##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779785_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	164885
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.39297692331019	34.0	34.0	34.0	34.0	34.0
2	33.55108105649392	34.0	34.0	34.0	34.0	34.0
3	33.695023804469784	34.0	34.0	34.0	34.0	34.0
4	33.43421778815538	34.0	34.0	34.0	34.0	34.0
5	33.489153046062405	34.0	34.0	34.0	34.0	34.0
6	37.07327531309701	38.0	37.0	38.0	36.0	38.0
7	37.20789034781818	38.0	38.0	38.0	36.0	38.0
8	37.0333019983625	38.0	37.0	38.0	36.0	38.0
9	36.94827910361767	38.0	37.0	38.0	35.0	38.0
10-11	36.874054644145914	38.0	37.0	38.0	35.0	38.0
12-13	37.06864784546805	38.0	37.5	38.0	35.5	38.0
14-15	37.112138763380536	38.0	38.0	38.0	36.0	38.0
16-17	37.013849046305	38.0	37.5	38.0	35.5	38.0
18-19	36.87883070018498	38.0	37.0	38.0	34.5	38.0
20-21	36.88433756860842	38.0	37.0	38.0	34.5	38.0
22-23	36.83015435000152	38.0	37.0	38.0	34.5	38.0
24-25	37.08296085150256	38.0	37.5	38.0	36.0	38.0
26-27	36.96429632774358	38.0	38.0	38.0	35.5	38.0
28-29	36.35577826970312	38.0	37.0	38.0	34.0	38.0
30-31	36.71794887345726	38.0	37.0	38.0	34.5	38.0
32-33	36.801531370349025	38.0	37.0	38.0	34.5	38.0
34-35	36.917824544379414	38.0	37.0	38.0	35.0	38.0
36-37	36.872956909361065	38.0	37.5	38.0	35.5	38.0
38-39	36.45450768717591	38.0	37.0	38.0	34.0	38.0
40-41	36.64475543560663	38.0	37.0	38.0	34.0	38.0
42-43	36.950198623282894	38.0	37.5	38.0	35.5	38.0
44-45	36.658771264820935	38.0	37.0	38.0	34.5	38.0
46-47	36.83235588440428	38.0	37.0	38.0	35.0	38.0
48-49	36.39979379567578	38.0	37.0	38.0	34.0	38.0
50-51	36.63581587166813	38.0	37.0	38.0	34.0	38.0
52-53	36.545495345240624	38.0	37.0	38.0	34.0	38.0
54-55	36.53053340206811	38.0	37.0	38.0	34.0	38.0
56-57	36.38609333778088	38.0	37.0	38.0	34.0	38.0
58-59	36.53100645904722	38.0	37.0	38.0	34.0	38.0
60-61	36.118258179943595	38.0	37.0	38.0	34.0	38.0
62-63	35.90738696667374	38.0	37.0	38.0	32.0	38.0
64-65	36.300324468569	38.0	37.0	38.0	34.0	38.0
66-67	36.08794917669891	38.0	37.0	38.0	32.5	38.0
68-69	36.208742456863874	38.0	37.0	38.0	34.0	38.0
70-71	35.6557297510386	38.0	37.0	38.0	30.5	38.0
72-73	34.72002607878218	38.0	36.5	38.0	23.5	38.0
74-75	34.57892773751402	38.0	36.5	38.0	23.5	38.0
76	34.2517087667162	38.0	35.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	10.0
23	19.0
24	41.0
25	86.0
26	192.0
27	377.0
28	584.0
29	1063.0
30	1734.0
31	2729.0
32	4481.0
33	6918.0
34	11168.0
35	19580.0
36	29472.0
37	86429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.48164508573717	18.72201254808684	11.609407424486973	32.18693494168902
2	18.058646935743095	18.57294477969494	39.677350880917004	23.69105740364496
3	17.82393789610941	21.09652181823695	35.473815083239835	25.605725202413804
4	12.771931952573004	23.69772872001698	35.76614003699548	27.76419929041453
5	12.593019377141646	33.418443157352094	40.45850137974952	13.53003608575674
6	26.850835430754767	42.02989962701277	17.700215301573824	13.41904964065864
7	25.158140522182126	27.976468447705976	21.71756072414107	25.14783030597083
8	22.461109257967674	40.835733996421745	18.258179943597053	18.444976802013525
9	22.516905722169998	15.922612730084603	19.59304970130697	41.96743184643842
10-11	23.39539679170331	27.774509506625826	28.18600843011796	20.6440852715529
12-13	22.707644722079024	21.348515632107226	28.522303423598267	27.42153622221548
14-15	21.034660520969158	22.092064165933834	22.235497467932195	34.63777784516481
16-17	25.705794947994054	28.692421991084693	22.784668102010492	22.817114958910757
18-19	25.69882038996877	25.143281681171725	23.919398368559904	25.238499560299605
20-21	21.124723291991387	24.01037086454195	23.581587166813232	31.283318676653426
22-23	21.346696182187586	28.476210692300697	23.889377444885827	26.28771568062589
24-25	25.641593255803375	28.57337801161433	23.551923339347717	22.233105393234574
26-27	25.480789641265122	29.286775631500745	24.007035206355944	21.225399520878188
28-29	21.06589441125633	29.330442429572123	24.500409376231918	25.10325378293962
30-31	20.76295599963611	28.97201079540286	29.12605755526579	21.13897564969524
32-33	24.18443157352094	30.11887072808321	24.889771659035084	20.806926039360764
34-35	22.047790884555905	24.584407314188677	32.94720562816508	20.420596173090335
36-37	21.155047457318737	32.241562300997664	25.449555750977954	21.153834490705645
38-39	20.48700609515723	25.70670467295388	28.863753525184222	24.94253570670467
40-41	20.683809928131726	23.19434757558298	29.844740273523975	26.277102222761318
42-43	25.125695189922553	23.175524462800894	29.328675485040907	22.370104862235646
44-45	20.311429177911876	27.91430390878491	29.9748309427783	21.79943597052491
46-47	24.82609091184765	23.08972920520363	26.48270006368075	25.601479819267976
48-49	24.23234375473815	22.78891348515632	25.89683718955636	27.081905570549168
50-51	20.43109566356705	23.078509514190916	30.184581976112923	26.30581284612912
52-53	19.022095224228305	22.99037808192584	35.08845369831101	22.899072995534848
54-55	18.523788863031612	23.47513706547892	31.29310318671549	26.707970884773975
56-57	23.07825337686137	23.24360732533162	26.750324639862132	26.927814657944875
58-59	19.014639947094764	23.42512877450356	34.094149481565076	23.466081796836608
60-61	23.105810122972947	27.727457487274997	26.500154702031754	22.6665776877203
62-63	18.47114244201037	33.3726654755802	24.788349597022485	23.367842485386948
64-65	18.513845500591426	33.96590943556459	23.897364350489823	23.62288071335416
66-67	18.563112215335984	33.8205270550262	23.597952936965143	24.01840779267268
68-69	18.49529020009826	34.008515851979446	23.238753191928136	24.257440755994153
70-71	18.730229976738475	33.19271280354473	23.428027258748852	24.649029960967948
72-73	19.036165772935725	31.45245432266518	23.11163953121588	26.399740373183217
74-75	19.311771342610005	31.24962088585328	23.432590471799976	26.006017299736744
76	19.50311161792001	31.578372739072947	23.29406912280276	25.624446520204287
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.5
15	8.0
16	16.0
17	19.0
18	24.0
19	31.5
20	71.5
21	109.0
22	135.5
23	237.5
24	398.0
25	483.0
26	533.5
27	639.5
28	890.0
29	1085.0
30	1278.0
31	1627.0
32	2120.5
33	2458.0
34	2830.5
35	3590.5
36	5990.0
37	8002.0
38	9453.0
39	11181.5
40	10772.0
41	9840.0
42	9595.0
43	9396.5
44	9471.5
45	9605.5
46	9466.0
47	9634.5
48	9304.5
49	8379.5
50	7953.0
51	7433.0
52	6282.0
53	5266.5
54	4882.0
55	4676.5
56	4025.0
57	3155.0
58	2731.0
59	2429.5
60	1988.5
61	1665.5
62	1482.0
63	1352.0
64	1163.5
65	1062.0
66	976.5
67	934.0
68	867.5
69	782.5
70	719.0
71	674.0
72	600.0
73	517.5
74	475.5
75	442.0
76	426.0
77	350.0
78	291.0
79	292.0
80	261.0
81	201.0
82	138.5
83	105.0
84	97.5
85	82.5
86	57.0
87	39.0
88	32.0
89	16.5
90	9.0
91	7.0
92	4.0
93	6.0
94	7.5
95	3.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.047912181217211997
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0015162082663674682
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0012129666130939745
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.01576856597022167
52-53	0.032143615246990324
54-55	0.05549322254904934
56-57	0.052764047669587893
58-59	0.037601965005913215
60-61	0.03153713194044334
62-63	0.030020923674075873
64-65	0.01819449919640962
66-67	0.03851168996573369
68-69	0.009703732904751796
70-71	0.012432907784213241
72-73	0.02001394911605058
74-75	0.016375049276768656
76	0.011523182824392759
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	164885.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.70139794402159
#Duplication Level	Percentage of deduplicated	Percentage of total
1	44.535972502074195	13.673166146101828
2	18.088973173718937	11.107135276101525
3	13.130654656078386	12.093883615853473
4	8.211844652522618	10.084604421263304
5	5.061040654260993	7.769051156866907
6	2.915728339457153	5.371016162780119
7	1.813440796491644	3.8972617278709403
8	1.177353719726601	2.8917124056160355
9	0.8533839042313619	2.3580070958546866
>10	4.152344830310932	20.991600206204325
>50	0.03950851408478527	0.7223216180974619
>100	0.013827979929674845	0.8533220123116111
>500	0.0019754257042392635	0.48943202838341876
>1k	0.0019754257042392635	0.9254935257907027
>5k	0.0	0.0
>10k+	0.0019754257042392635	6.77199260090366
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT	11166	6.77199260090366	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	1526	0.9254935257907027	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	807	0.48943202838341876	No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT	440	0.2668526548806744	RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	286	0.17345422567243837	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0012129666130939747	0.0
2	0.0	0.0	0.0	0.0012129666130939747	0.0
3	0.0	0.0	0.0	0.0012129666130939747	0.0
4	0.0	0.0	0.0	0.0036388998392819236	0.0
5	0.0	0.0	0.0	0.004245383145828911	0.0
6	0.0	0.0	0.0	0.004245383145828911	0.0
7	0.0	0.0	0.0	0.004245383145828911	0.0
8	0.0	0.0	0.0	0.004245383145828911	0.0
9	0.0	0.0	0.0	0.00667131637201686	0.0
10	0.0	0.0	0.0	0.007884282985110834	0.0
11	0.0	0.0	0.0	0.007884282985110834	0.0
12	0.0	0.0	0.0	0.008490766291657821	0.0
13	0.0	0.0	0.0	0.009097249598204809	0.0
14	0.0	0.0	0.0	0.009097249598204809	0.0
15	0.0	0.0	0.0	0.01334263274403372	0.0
16	0.0	0.0	0.0	0.013949116050580707	0.0
17	0.0	0.0	0.0	0.018800982502956608	0.0
18	0.0	0.0	0.0	0.018800982502956608	0.0
19	0.0	0.0	0.0	0.02062043242259757	0.0
20	0.0	0.0	0.0	0.02243988234223853	0.0
21	0.0	0.0	0.0	0.02486581556842648	0.0
22	0.0	0.0	0.0	0.026078782181520453	0.0
23	0.0	0.0	0.0	0.0285047154077084	0.0
24	0.0	0.0	0.0	0.02911119871425539	0.0
25	0.0	0.0	0.0	0.030324165327349363	0.0
26	0.0	0.0	0.0	0.030930648633896354	0.0
27	0.0	0.0	0.0	0.041847348151742125	0.0
28	0.0	0.0	0.0	0.05761591412196379	0.0
29	0.0	0.0	0.0	0.07702337993146739	0.0
30	0.0	0.0	0.0	0.10370864541953483	0.0
31	0.0	0.0	0.0	0.13221336082724322	0.0
32	0.0	0.0	0.0	0.1667829093004215	0.0
33	0.0	0.0	0.0	0.20256542438669375	0.0
34	0.0	0.0	0.0	0.26139430512175155	0.0
35	0.0	0.0	0.0	0.3626770173150984	0.0
36	0.0	0.0	0.0	0.4961033447554356	0.0
37	0.0	0.0	0.0	0.7702337993146738	0.0
38	0.0	0.0	0.0	1.1098644509809867	0.0
39	0.0	0.0	0.0	1.4725414682960851	0.0
40	0.0	0.0	0.0	1.9977560117657762	0.0
41	0.0	0.0	0.0	2.541771537738424	0.0
42	0.0	0.0	0.0	3.14643539436577	0.0
43	0.0	0.0	0.0	3.8232707644722077	0.0
44	0.0	0.0	0.0	4.491615368286988	0.0
45	0.0	0.0	0.0	5.2679140006671314	0.0
46	0.0	0.0	0.0	5.9999393516693456	0.0
47	0.0	0.0	0.0	6.775631500742942	0.0
48	0.0	0.0	0.0	7.439730721411893	0.0
49	0.0	0.0	0.0	8.109288291839768	0.0
50	0.0	0.0	0.0	8.791582011705128	0.0
51	0.0	0.0	0.0	9.430815416805652	0.0
52	0.0	0.0	0.0	10.169512084179884	0.0
53	0.0	0.0	0.0	10.875458653000576	0.0
54	0.0	0.0	0.0	11.567456105770688	0.0
55	0.0	0.0	0.0	12.178184795463505	0.0
56	0.0	0.0	0.0	12.824089516936047	0.0
57	0.0	0.0	0.0	13.387512508718197	0.0
58	0.0	0.0	0.0	13.979440215908058	0.0
59	0.0	0.0	0.0	14.576219789550292	0.0
60	0.0	0.0	0.0	15.165115080207418	0.0
61	0.0	0.0	0.0	15.722473238924099	0.0
62	0.0	0.0	0.0	16.267095248203294	0.0
63	0.0	0.0	0.0	16.868120204991357	0.0
64	0.0	0.0	0.0	17.536464808806137	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGA	15	0.002217584	70.03126	49
CGTCAAT	15	0.0022229294	69.98878	32
GACATTA	20	0.0069292793	52.523445	55
TCCGCGC	20	0.0069459337	52.491585	13
GTCAATC	20	0.0069459337	52.491585	33
GATGCGT	20	0.0069459337	52.491585	5
TTTGACC	40	7.453182E-7	52.491585	38
AGGATGC	20	0.0069459337	52.491585	3
CTCGTAT	1960	0.0	51.777412	39
TCGTATG	1995	0.0	51.74609	40
TATGCCG	1975	0.0	51.738537	43
CGTATGC	1990	0.0	51.700253	41
ATCTCGT	1875	0.0	51.698376	37
TCTCGTA	1875	0.0	51.698376	38
GCCGTCT	1980	0.0	51.623543	46
ATGCCGT	1985	0.0	51.47789	44
GTATGCC	1995	0.0	51.219856	42
CGTCTTC	1990	0.0	51.188225	48
CCGTCTT	1990	0.0	51.188225	47
TGCCGTC	1990	0.0	51.188225	45
>>END_MODULE