FastQCFastQC Report
Wed 25 May 2016
SRR1779784_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779784_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21612
Sequences flagged as poor quality0
Sequence length76
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG252.713652E-670.1461735
AATGTAC150.002177229470.1461735
TGTACAT150.002177229470.1461737
TAGTGTA207.673997E-570.14616434
GTGTACA207.673997E-570.14616436
TAGGTGT207.673997E-570.14616433
CATCTGC207.7630015E-569.9837959
TGCTGCC150.002197403269.98379516
TTATACC252.7514834E-669.9837952
TAGTAAA150.002197403269.98379523
GATCTCT252.7514834E-669.98379541
GCGCCAA150.002197403269.98379518
ATATGGC150.002197403269.98379510
TTTATAC150.002197403269.9837951
AGCGACC150.002197403269.98379527
TATGGCG150.002197403269.98379511
GAAGGCT207.7630015E-569.98379526
ACATCTT401.00462785E-861.235828
GGTGTAG352.7996066E-760.1252935
TTCCGAC352.845136E-759.98611519
Produced by FastQC (version 0.11.5)