##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779784_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21612
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.89797334813992	23.0	12.0	32.0	12.0	34.0
2	22.41768461965575	23.0	12.0	33.0	12.0	34.0
3	22.74389228206552	23.0	11.0	33.0	11.0	34.0
4	23.28317601332593	23.0	12.0	33.0	12.0	34.0
5	21.173931149361465	21.0	11.0	33.0	11.0	34.0
6	24.18040903201925	26.0	12.0	34.0	11.0	38.0
7	24.529381824912086	26.0	12.0	36.0	11.0	38.0
8	24.54668702572645	26.0	11.0	36.0	11.0	38.0
9	24.49088469368869	26.0	11.0	37.0	11.0	38.0
10-11	23.71823523968166	24.5	11.5	34.0	11.0	38.0
12-13	23.629765870812513	25.5	11.0	35.5	11.0	38.0
14-15	22.959605774569685	23.0	11.0	34.0	11.0	38.0
16-17	22.99655284101425	23.0	11.0	34.0	11.0	38.0
18-19	22.847260781047567	22.5	11.0	34.0	11.0	38.0
20-21	23.04839903757172	23.0	11.0	34.0	10.5	38.0
22-23	23.10447899315195	22.5	11.0	35.0	10.5	38.0
24-25	23.35163335184157	23.0	11.0	35.0	10.0	38.0
26-27	22.683092726263187	22.5	11.0	34.0	10.0	38.0
28-29	22.67161761984083	22.0	11.0	34.0	10.5	38.0
30-31	22.657320007403293	21.5	11.0	34.5	11.0	38.0
32-33	22.947644826947993	22.5	11.0	35.5	11.0	38.0
34-35	23.140824541921155	23.0	11.0	35.5	11.0	38.0
36-37	22.9269618730335	22.5	11.0	34.0	11.0	38.0
38-39	22.714950027762356	21.0	11.0	34.0	11.0	38.0
40-41	22.727119193040906	22.0	11.0	34.0	11.0	38.0
42-43	22.92094669627984	22.5	11.0	34.5	11.0	38.0
44-45	23.053211178974642	23.0	11.0	34.5	11.0	38.0
46-47	22.86424208772904	22.0	11.0	34.5	11.0	38.0
48-49	22.610910605219324	21.5	11.0	34.0	11.0	38.0
50-51	22.41245604293911	21.0	11.0	34.0	11.0	38.0
52-53	22.85614473440681	23.0	11.0	34.0	11.0	38.0
54-55	22.760572829909307	22.5	11.0	34.0	11.0	38.0
56-57	22.634670553396262	22.0	11.0	34.0	11.0	38.0
58-59	22.51915602443087	21.5	11.0	34.0	11.0	38.0
60-61	22.859291134554876	23.0	11.0	34.0	11.0	38.0
62-63	22.532366277993706	21.5	11.0	34.0	11.0	38.0
64-65	22.463330557097912	22.0	11.0	34.0	11.0	38.0
66-67	22.850916157690172	22.5	11.0	34.0	11.0	38.0
68-69	22.742781787895616	22.5	11.0	34.0	10.5	38.0
70-71	22.635410882842866	22.5	11.0	34.0	11.0	38.0
72-73	22.329284656672222	21.5	11.0	34.0	10.0	38.0
74-75	22.516703683139	22.5	11.0	34.0	10.5	38.0
76	21.935915232278365	21.0	11.0	33.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
12	29.0
13	226.0
14	942.0
15	1732.0
16	2217.0
17	2484.0
18	2292.0
19	1931.0
20	1376.0
21	928.0
22	665.0
23	436.0
24	323.0
25	258.0
26	189.0
27	164.0
28	162.0
29	134.0
30	156.0
31	140.0
32	205.0
33	245.0
34	399.0
35	577.0
36	1121.0
37	2281.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.979640940218395	22.33481399222654	8.69887099759393	48.98667406996113
2	12.257079400333149	22.950212844715896	36.81750879141218	27.975198963538773
3	10.947621691652785	20.035165648713676	45.363686840644085	23.65352581898945
4	8.08347214510457	45.271145659818615	18.8321302979826	27.813251897094204
5	21.969276327965943	30.30723672034055	27.961317786414952	19.762169165278547
6	14.598371275217472	39.51045715343328	11.183601702757727	34.70756986859153
7	13.321302979826022	40.509901906348325	12.261706459374421	33.90708865445123
8	11.605738084220269	52.429430819065246	10.39796390559926	25.56686719111522
9	17.249676105867113	25.356283546178048	12.243198223209328	45.15084212474551
10-11	13.569164565162067	36.318626657103856	19.811211623441224	30.30099715429285
12-13	13.543401813807144	25.814362391264112	24.55348880251712	36.08874699241162
14-15	26.562246951854334	28.89202507924022	17.263956689725376	27.281771279180067
16-17	28.76179900055525	25.41180825467333	15.676476031834166	30.14991671293726
18-19	19.047288543401812	24.571997038682213	21.145659818619286	35.23505459929669
20-21	26.374236535258188	23.540162872478255	17.959929668702575	32.12567092356098
22-23	29.38182491208588	22.931704608550803	15.970294280955025	31.71617619840829
24-25	18.612344993522118	32.82204330927262	15.22071071626874	33.344900980936515
26-27	23.810845826392747	33.4513233388858	17.77484730705164	24.962983527669813
28-29	20.135579103234463	30.445143676831243	21.62324742029522	27.796029799639072
30-31	22.311678697020174	28.34305015732001	22.024801036461223	27.32047010919859
32-33	16.738386081806404	32.01693503609106	22.263094577086804	28.981584305015733
34-35	15.130833121254888	37.56796150197811	19.90838211137589	27.392823265391108
36-37	23.435367066935054	26.50100645519539	20.95971865528331	29.103907822586244
38-39	25.23887744950605	22.726326261481155	25.02371422622215	27.011082062790642
40-41	18.8321302979826	29.747362576346475	21.754118082546732	29.66638904312419
42-43	12.835461780492318	29.0741254858412	24.523412918748843	33.56699981491764
44-45	13.157651196150107	24.124288556753505	29.75567997778909	32.962380269307296
46-47	16.92809550249861	28.31991486211364	24.983805293355545	29.768184342032207
48-49	18.42263557282991	29.807514343883028	21.557468073292615	30.21238200999445
50-51	26.098926522302424	29.182861373311127	16.45382195076809	28.26439015361836
52-53	11.892367136345758	33.685014229194145	21.096226371439812	33.326392263020296
54-55	15.536069594188145	26.902966082087826	21.465920133265467	36.095044190458566
56-57	18.916292628753876	29.92226181111471	18.374901670445606	32.78654388968581
58-59	17.23276260990282	29.990745025451183	19.37528921795465	33.401203146691344
60-61	13.489437515907355	26.585529512482935	21.25222702977857	38.67280594183114
62-63	17.454881999074505	28.172142526608052	19.664507172605276	34.70846830171217
64-65	17.691702531352675	26.884631403581842	18.968948123467076	36.45471794159841
66-67	13.878797695351366	35.96732767198093	15.204664830969294	34.94920980169841
68-69	15.801406625948546	39.34851008698871	13.568850638534148	31.281232648528594
70-71	16.324264297612437	36.54913936701833	13.446233573940402	33.68036276142883
72-73	18.68261533478321	35.90995326454121	14.585164962287726	30.822266438387857
74-75	15.131883387320686	34.31050439611291	16.647385469689958	33.91022674687645
76	13.284286507495835	33.87932630020359	17.69850083287063	35.13788635942995
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	3.0
17	5.0
18	3.0
19	1.0
20	5.5
21	10.0
22	14.5
23	24.0
24	32.5
25	36.0
26	34.0
27	44.0
28	75.0
29	94.0
30	85.0
31	112.0
32	162.0
33	176.0
34	200.5
35	275.5
36	345.5
37	365.0
38	427.5
39	546.5
40	708.5
41	864.0
42	914.0
43	969.0
44	1119.5
45	1248.0
46	1281.0
47	1341.0
48	1376.5
49	1339.5
50	1327.0
51	1330.5
52	1233.5
53	1124.0
54	1115.0
55	1060.0
56	923.5
57	789.5
58	737.0
59	715.0
60	611.5
61	481.0
62	432.0
63	391.5
64	297.5
65	248.5
66	222.5
67	192.0
68	162.0
69	119.0
70	97.5
71	89.0
72	80.0
73	61.0
74	47.5
75	44.0
76	44.5
77	39.0
78	37.0
79	41.0
80	35.0
81	21.5
82	15.0
83	16.0
84	15.5
85	13.0
86	10.0
87	9.0
88	7.5
89	4.5
90	1.5
91	1.5
92	3.0
93	3.0
94	3.0
95	2.5
96	2.0
97	1.5
98	1.5
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.009254118082546734
9	0.0
10-11	0.0023135295206366834
12-13	0.0
14-15	0.0023135295206366834
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.004627059041273367
30-31	0.0
32-33	0.0
34-35	0.0023135295206366834
36-37	0.006940588561910051
38-39	0.0023135295206366834
40-41	0.0
42-43	0.0
44-45	0.004627059041273367
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.006940588561910051
54-55	0.004627059041273367
56-57	0.004627059041273367
58-59	0.009254118082546734
60-61	0.011567647603183417
62-63	0.009254118082546734
64-65	0.013881177123820101
66-67	0.016194706644456783
68-69	0.0
70-71	0.0
72-73	0.004627059041273367
74-75	0.009254118082546734
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	21612.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.30760688506386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.41520130681273	93.81824912085878
2	1.748822907658307	3.3684989820470106
3	0.5957528586528298	1.7212659633536922
4	0.13932929758816182	0.5367388487877105
5	0.05284904391275103	0.25448824727003516
6	0.04324012683770539	0.2498611882287618
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0048044585375228216	0.05089764945400703
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004627059041273367	0.0
2	0.0	0.0	0.0	0.004627059041273367	0.0
3	0.0	0.0	0.0	0.004627059041273367	0.0
4	0.0	0.0	0.0	0.009254118082546734	0.0
5	0.0	0.0	0.0	0.0138811771238201	0.0
6	0.0	0.0	0.0	0.0138811771238201	0.0
7	0.0	0.0	0.0	0.0138811771238201	0.0
8	0.0	0.0	0.0	0.0138811771238201	0.0
9	0.0	0.0	0.0	0.0138811771238201	0.0
10	0.0	0.0	0.0	0.0138811771238201	0.0
11	0.0	0.0	0.0	0.0138811771238201	0.0
12	0.0	0.0	0.0	0.0138811771238201	0.0
13	0.0	0.0	0.0	0.0138811771238201	0.0
14	0.0	0.0	0.0	0.018508236165093468	0.0
15	0.0	0.0	0.0	0.018508236165093468	0.0
16	0.0	0.0	0.0	0.018508236165093468	0.0
17	0.0	0.0	0.0	0.018508236165093468	0.0
18	0.0	0.0	0.0	0.037016472330186935	0.0
19	0.0	0.0	0.0	0.050897649454007036	0.0
20	0.0	0.0	0.0	0.050897649454007036	0.0
21	0.0	0.0	0.0	0.050897649454007036	0.0
22	0.0	0.0	0.0	0.17582824356838794	0.0
23	0.0	0.0	0.0	0.17582824356838794	0.0
24	0.0	0.0	0.0	0.1804553026096613	0.0
25	0.0	0.0	0.0	0.19896353877475476	0.0
26	0.0	0.0	0.0	0.19896353877475476	0.0
27	0.0	0.0	0.0	0.19896353877475476	0.0
28	0.0	0.0	0.0	0.21747177493984823	0.0
29	0.0	0.0	0.0	0.24523412918748844	0.0
30	0.0	0.0	0.0	0.277623542476402	0.0
31	0.0	0.0	0.0	0.2822506015176754	0.0
32	0.0	0.0	0.0	0.31001295576531557	0.0
33	0.0	0.0	0.0	0.328521191930409	0.0
34	0.0	0.0	0.0	0.3840459004256894	0.0
35	0.0	0.0	0.0	0.43494354987969647	0.0
36	0.0	0.0	0.0	0.4904682583749769	0.0
37	0.0	0.0	0.0	0.5968906163242643	0.0
38	0.0	0.0	0.0	0.694058856191005	0.0
39	0.0	0.0	0.0	0.8421247455117528	0.0
40	0.0	0.0	0.0	0.9901906348325005	0.0
41	0.0	0.0	0.0	1.198408291689802	0.0
42	0.0	0.0	0.0	1.314084767721636	0.0
43	0.0	0.0	0.0	1.5223024245789376	0.0
44	0.0	0.0	0.0	1.7258930223949658	0.0
45	0.0	0.0	0.0	1.9479918563760874	0.0
46	0.0	0.0	0.0	2.109938922820655	0.0
47	0.0	0.0	0.0	2.332037756801777	0.0
48	0.0	0.0	0.0	2.4523412918748844	0.0
49	0.0	0.0	0.0	2.5957801221543586	0.0
50	0.0	0.0	0.0	2.7484730705163796	0.0
51	0.0	0.0	0.0	2.9335554321673145	0.0
52	0.0	0.0	0.0	3.104756616694429	0.0
53	0.0	0.0	0.0	3.234314269850083	0.0
54	0.0	0.0	0.0	3.4147695724597447	0.0
55	0.0	0.0	0.0	3.5628354617804923	0.0
56	0.0	0.0	0.0	3.71090135110124	0.0
57	0.0	0.0	0.0	3.858967240421988	0.0
58	0.0	0.0	0.0	3.997779011660189	0.0
59	0.0	0.0	0.0	4.15047196002221	0.0
60	0.0	0.0	0.0	4.358689616879511	0.0
61	0.0	0.0	0.0	4.492874329076439	0.0
62	0.0	0.0	0.0	4.613177864149547	0.0
63	0.0	0.0	0.0	4.756616694429021	0.0
64	0.0	0.0	0.0	4.886174347584675	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGTAG	25	2.713652E-6	70.14617	35
AATGTAC	15	0.0021772294	70.14617	35
TGTACAT	15	0.0021772294	70.14617	37
TAGTGTA	20	7.673997E-5	70.146164	34
GTGTACA	20	7.673997E-5	70.146164	36
TAGGTGT	20	7.673997E-5	70.146164	33
CATCTGC	20	7.7630015E-5	69.983795	9
TGCTGCC	15	0.0021974032	69.983795	16
TTATACC	25	2.7514834E-6	69.983795	2
TAGTAAA	15	0.0021974032	69.983795	23
GATCTCT	25	2.7514834E-6	69.983795	41
GCGCCAA	15	0.0021974032	69.983795	18
ATATGGC	15	0.0021974032	69.983795	10
TTTATAC	15	0.0021974032	69.983795	1
AGCGACC	15	0.0021974032	69.983795	27
TATGGCG	15	0.0021974032	69.983795	11
GAAGGCT	20	7.7630015E-5	69.983795	26
ACATCTT	40	1.00462785E-8	61.23582	8
GGTGTAG	35	2.7996066E-7	60.12529	35
TTCCGAC	35	2.845136E-7	59.986115	19
>>END_MODULE