SRR1779784_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1779784_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21612
Sequences flagged as poor quality	0
Sequence length	76
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	9700	44.882472700351656	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	1398	6.468628539700166	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	619	2.8641495465482136	No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT	413	1.9109753840459005	Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	247	1.1428835831945217	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT	202	0.9346659263372201	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT	180	0.8328706274292059	No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	163	0.7542106237275588	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	137	0.6339070886544512	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT	132	0.6107717934480844	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAGCTCGTATGCCGT	105	0.4858411993337035	No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	73	0.33777531001295574	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	67	0.31001295576531557	No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT	49	0.226725893022395	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCG	48	0.22209883398112162	No Hit
CTTATACACATCTCCGAGCCCACGAGAATTGCTAAGATCTCGTATGCCGT	43	0.19896353877475476	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCAGT	41	0.18970942069220803	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCG	39	0.18045530260966128	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	39	0.18045530260966128	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTACGATCTCGTATGCCGT	37	0.17120118452711455	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCG	37	0.17120118452711455	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	37	0.17120118452711455	No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	35	0.16194706644456783	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTCTGCCGT	34	0.15732000740329447	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCCGT	32	0.14806588932074774	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGATAAGATCTCGTATGCCGT	31	0.14343883027947438	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCTAAGATCTCGTATGCCGT	31	0.14343883027947438	No Hit
CTTATACACCTCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	30	0.13881177123820101	No Hit
TCTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	30	0.13881177123820101	No Hit
TCCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	29	0.13418471219692762	No Hit
GTGTATAAGAGACAGGCAGCGTCAGATGTGTATAAGCTGTCTCTTATACA	26	0.12030353507310754	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTCAGATCTCGTATGCCGT	26	0.12030353507310754	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCC	25	0.11567647603183417	No Hit
CTTCTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	24	0.11104941699056081	No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCTAAGATCTCGTATGCCGT	22	0.10179529890801406	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTCTT	20	7.696665E-5	70.10441	47
TTGCAAA	15	0.002182378	70.10441	58
TTCTGCC	15	0.002182378	70.10441	52
CCTGAAA	15	0.002182378	70.10441	57
ACACACC	15	0.002182378	70.10441	70
TGCTTGC	20	7.696665E-5	70.10441	55
GACAAAA	20	7.696665E-5	70.10441	60
GCTTGCA	20	7.696665E-5	70.10441	56
GCTTGAC	20	7.696665E-5	70.10441	56
GAACAAA	20	7.696665E-5	70.10441	60
CTTGCAA	15	0.002182378	70.10441	57
TTGAACA	20	7.696665E-5	70.10441	58
AGTCTTC	20	7.696665E-5	70.10441	48
CTTGACA	20	7.696665E-5	70.10441	57
CTTGAAC	20	7.696665E-5	70.10441	57
TGAACAA	20	7.696665E-5	70.10441	59
ACACGAG	20	7.7859324E-5	69.94213	20
CTTTATA	35	3.5124685E-9	69.94213	2
CACACGA	20	7.7859324E-5	69.94213	19
TATGCAG	20	7.7859324E-5	69.94213	43

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content