FastQCFastQC Report
Wed 25 May 2016
SRR1779783_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779783_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128933
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTG150.002220755469.99728411
TAGATCC150.002220755469.99728439
GGTGGAC352.9559305E-759.9976747
GTGGACG252.3835104E-455.9978348
TGTACGG252.3835104E-455.9978354
ATTTGAC252.3835104E-455.9978335
AATGTGC252.3835104E-455.9978345
GCCGTAT452.6793714E-854.44233354
TGGACGC200.00693917652.49796749
TGACCAG200.00693917652.49796716
CTCGGCG200.00693917652.49796744
ACGTGTA200.00693917652.49796734
TCCGTAT200.00693917652.49796713
GCGTAGA200.00693917652.49796736
TGCGTTG200.00693917652.49796730
GTAGATC1250.050.3980538
TCGGCGG352.077555E-549.9980645
GGCGGTC352.077555E-549.9980647
CCGACAA352.077555E-549.9980621
ATCTCGG850.049.40984742
Produced by FastQC (version 0.11.5)