##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779783_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	128933
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.533494140367477	34.0	27.0	34.0	12.0	34.0
2	30.104294478527606	34.0	31.0	34.0	12.0	34.0
3	29.886157926985334	34.0	31.0	34.0	11.0	34.0
4	29.913869994493265	34.0	31.0	34.0	12.0	34.0
5	30.274592230072983	34.0	32.0	34.0	11.0	34.0
6	33.28321686457307	37.0	34.0	38.0	21.0	38.0
7	33.49386115269171	38.0	34.0	38.0	21.0	38.0
8	32.882070532757325	38.0	34.0	38.0	11.0	38.0
9	33.797049630428205	38.0	34.0	38.0	21.0	38.0
10-11	32.39368121427408	37.0	30.5	38.0	16.5	38.0
12-13	33.28502400471562	38.0	34.0	38.0	16.0	38.0
14-15	31.94385456012037	37.0	29.0	38.0	11.0	38.0
16-17	32.61578494256707	37.5	31.0	38.0	16.0	38.0
18-19	33.47210178930142	38.0	34.0	38.0	21.0	38.0
20-21	32.17853458773161	37.0	30.0	38.0	16.0	38.0
22-23	33.10076939185468	38.0	33.0	38.0	16.0	38.0
24-25	33.438146168940456	38.0	34.0	38.0	21.0	38.0
26-27	32.32452126298155	38.0	31.5	38.0	11.0	38.0
28-29	33.60228956124499	38.0	34.5	38.0	21.0	38.0
30-31	33.974463481032785	38.0	36.0	38.0	21.0	38.0
32-33	34.07970030946305	38.0	36.0	38.0	21.0	38.0
34-35	34.05318653874493	38.0	36.0	38.0	21.0	38.0
36-37	33.55529616157229	38.0	34.5	38.0	16.0	38.0
38-39	33.49769647801571	38.0	34.5	38.0	16.0	38.0
40-41	33.48968844283465	38.0	34.0	38.0	16.0	38.0
42-43	33.87122769190199	38.0	35.0	38.0	21.0	38.0
44-45	33.96189105969767	38.0	35.5	38.0	21.0	38.0
46-47	33.86601568256381	38.0	35.0	38.0	21.0	38.0
48-49	33.60074224597271	38.0	34.5	38.0	16.0	38.0
50-51	32.96917779001497	37.5	32.5	38.0	15.5	38.0
52-53	33.557436808264754	38.0	34.0	38.0	21.0	38.0
54-55	33.68618972644707	38.0	34.5	38.0	21.0	38.0
56-57	33.71001217686705	38.0	34.5	38.0	21.0	38.0
58-59	33.94829097283085	38.0	36.0	38.0	21.0	38.0
60-61	33.860799795242485	38.0	35.0	38.0	21.0	38.0
62-63	33.83588375357744	38.0	35.0	38.0	21.0	38.0
64-65	33.87201492247912	38.0	35.5	38.0	21.0	38.0
66-67	33.69129703024051	38.0	34.0	38.0	21.0	38.0
68-69	33.064766196396576	38.0	33.5	38.0	15.0	38.0
70-71	33.07687325975507	37.5	33.5	38.0	15.5	38.0
72-73	33.34884397322641	38.0	34.0	38.0	18.5	38.0
74-75	33.47057774192798	38.0	34.0	38.0	20.0	38.0
76	32.51514352415596	37.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	13.0
13	274.0
14	1044.0
15	2003.0
16	2646.0
17	2724.0
18	2569.0
19	2106.0
20	1654.0
21	1228.0
22	927.0
23	878.0
24	857.0
25	852.0
26	1046.0
27	1149.0
28	1389.0
29	1767.0
30	2222.0
31	2834.0
32	3801.0
33	5117.0
34	7799.0
35	12155.0
36	23001.0
37	46878.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.25759115199367	20.77668246298465	10.910317761938371	32.05540862308331
2	17.958164317901545	20.449380686092777	33.48095522480668	28.111499771198993
3	20.059255582356727	22.023066243707973	30.617452475316636	27.300225698618664
4	15.276926775922378	20.580456516175065	33.439848603538266	30.70276810436428
5	13.016062606159789	33.24750063986722	34.04171158663802	19.694725167334973
6	26.36583625476996	40.827723140880465	16.417956752396613	16.388483851952966
7	25.412054883771436	31.64271254275675	20.74119462951903	22.204037943952777
8	21.97428021841648	37.37434847356664	20.018925291635643	20.632446016381238
9	23.575905184449752	17.33889733486395	20.810710185845924	38.27448729484037
10-11	23.20889792056093	26.242350440940363	25.461688215994847	25.087063422503856
12-13	23.318015240532837	23.223004285187987	26.08884493824288	27.3701355360363
14-15	24.158858933391244	25.324201904880088	22.931079949120466	27.5858592126082
16-17	25.02191060473269	26.439313441865153	23.589771431673814	24.94900452172834
18-19	22.16745520825254	26.376716047467617	25.793841619483445	25.661987124796404
20-21	23.181032008872826	25.64044891532812	25.956504541118253	25.222014534680802
22-23	25.14377901272391	25.485048146094215	24.515145756822474	24.856027084359404
24-25	22.367864857423186	28.307886807233356	24.160691225117407	25.163557110226055
26-27	22.974214990130417	28.293706347944454	25.30607338005065	23.426005281874485
28-29	22.54833142657695	27.427042329991664	26.76232766477284	23.262298578658548
30-31	22.844810870762334	26.948492627954053	26.53005824730674	23.676638253976872
32-33	21.326192673714257	28.069229754989028	26.289235494404075	24.315342076892648
34-35	21.78421707819329	28.75192448644813	25.99850306948317	23.46535536587541
36-37	23.862605012682973	26.133128544056845	25.587799523709787	24.416466919550395
38-39	23.613136421457604	25.868341038130993	27.0799075424281	23.438614997983308
40-41	22.114456125927333	26.735902397784873	26.639727297053085	24.50991417923471
42-43	20.545634343551857	26.416147984410447	27.151028639016538	25.88718903302115
44-45	20.402164006903103	25.195555641736632	28.664559539275952	25.73772081208431
46-47	21.159461880625607	26.036508041153954	27.995315305532824	24.80871477268761
48-49	20.94623155527117	26.700405250809535	27.520601865317122	24.832761328602178
50-51	22.511478919120158	26.493035088263582	26.099028945490648	24.896457047125615
52-53	19.306696452811455	27.357875875549766	27.621607365865387	25.713820305773393
54-55	19.640488656195462	26.500678689160363	27.43377932906729	26.425053325576886
56-57	20.176700783828544	27.008536400904447	26.923986859915374	25.890775955351636
58-59	20.231225081832427	26.844138316191184	26.54512030530088	26.379516296675508
60-61	19.579299323527586	25.87662136163346	27.149273878234965	27.394805436603985
62-63	20.23146496222405	26.17555345258226	26.803859817868727	26.789121767324964
64-65	20.929510924584875	26.070058530795574	25.65658054481349	27.343849999806064
66-67	20.030484966955225	27.79329175587204	24.803360327655216	27.37286294951752
68-69	20.56704309248286	28.705829429466696	24.064623212235908	26.66250426581454
70-71	21.247634411938076	27.432289889243943	24.241072813576146	27.07900288524183
72-73	21.863098700795035	27.15028117122358	23.900329649020748	27.086290478960635
74-75	21.2228862628903	27.02783411996944	24.270018499199146	27.47926111794112
76	21.10912898472039	26.429070038005122	24.63507329558675	27.826727681687736
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	4.5
14	7.5
15	6.5
16	8.5
17	11.0
18	19.0
19	32.0
20	67.0
21	97.0
22	115.0
23	152.5
24	220.5
25	269.0
26	360.0
27	482.5
28	636.5
29	759.0
30	892.0
31	1178.0
32	1573.5
33	1816.0
34	2079.5
35	2707.5
36	3420.0
37	3768.0
38	4111.5
39	5130.0
40	6246.0
41	6907.5
42	7128.0
43	7305.0
44	7782.0
45	8121.5
46	8161.0
47	8149.5
48	7891.0
49	7553.0
50	7462.0
51	6906.0
52	5935.0
53	5203.0
54	4886.0
55	4691.5
56	4075.5
57	3365.0
58	3076.0
59	2679.5
60	2109.5
61	1797.0
62	1658.0
63	1473.0
64	1167.0
65	982.5
66	870.5
67	822.0
68	730.5
69	587.0
70	526.5
71	518.0
72	462.0
73	402.5
74	355.0
75	311.0
76	274.0
77	225.5
78	202.5
79	191.0
80	179.0
81	142.5
82	104.0
83	90.0
84	82.0
85	69.0
86	50.5
87	37.0
88	31.0
89	22.0
90	13.5
91	10.0
92	12.0
93	9.0
94	7.5
95	6.5
96	4.0
97	6.0
98	6.0
99	8.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	7.755966277058628E-4
7	0.004653579766235177
8	0.0038779831385293137
9	0.006980369649352764
10-11	0.003102386510823451
12-13	3.877983138529314E-4
14-15	7.755966277058628E-4
16-17	0.0
18-19	0.0023267898831175883
20-21	0.0
22-23	0.0019389915692646568
24-25	0.0019389915692646568
26-27	0.0011633949415587941
28-29	0.004265781452382245
30-31	0.0
32-33	0.0
34-35	0.0019389915692646568
36-37	0.015511932554117255
38-39	0.006980369649352764
40-41	0.0011633949415587941
42-43	3.877983138529314E-4
44-45	0.004265781452382245
46-47	0.0019389915692646568
48-49	3.877983138529314E-4
50-51	7.755966277058628E-4
52-53	0.010858352787882079
54-55	0.006204773021646902
56-57	0.011246151101735009
58-59	0.00853156290476449
60-61	0.022492302203470017
62-63	0.01163394941558794
64-65	0.020553310634205364
66-67	0.013185142670999668
68-69	7.755966277058628E-4
70-71	7.755966277058628E-4
72-73	0.006204773021646902
74-75	0.006592571335499834
76	0.0023267898831175883
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	128933.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.12968751211869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.75176607899748	49.7157438359458
2	9.56569813870631	12.077590686635695
3	4.785306222740955	9.062846594743005
4	2.5382394495976413	6.40953053136125
5	1.3428343264328275	4.23863557041254
6	0.8170035014435776	3.0946305445463924
7	0.5184593648258492	2.2911124382431187
8	0.38700165857853674	1.9545035018187742
9	0.26414398918852505	1.5007794746108445
>10	1.0283186927943977	9.611193410531051
>50	0.0012285766939001167	0.043433411151528316
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.755966277058627E-4	0.0
2	0.0	0.0	0.0	7.755966277058627E-4	0.0
3	0.0	0.0	0.0	7.755966277058627E-4	0.0
4	0.0	0.0	0.0	0.0015511932554117254	0.0
5	0.0	0.0	0.0	0.0031023865108234508	0.0
6	0.0	0.0	0.0	0.0031023865108234508	0.0
7	0.0	0.0	0.0	0.0031023865108234508	0.0
8	0.0	0.0	0.0	0.0031023865108234508	0.0
9	0.0	0.0	0.0	0.0038779831385293137	0.0
10	0.0	0.0	0.0	0.0054291763939410395	0.0
11	0.0	0.0	0.0	0.0054291763939410395	0.0
12	0.0	0.0	0.0	0.006980369649352764	0.0
13	0.0	0.0	0.0	0.006980369649352764	0.0
14	0.0	0.0	0.0	0.006980369649352764	0.0
15	0.0	0.0	0.0	0.00853156290476449	0.0
16	0.0	0.0	0.0	0.00853156290476449	0.0
17	0.0	0.0	0.0	0.009307159532470353	0.0
18	0.0	0.0	0.0	0.013185142670999666	0.0
19	0.0	0.0	0.0	0.013960739298705529	0.0
20	0.0	0.0	0.0	0.013960739298705529	0.0
21	0.0	0.0	0.0	0.014736335926411392	0.0
22	0.0	0.0	0.0	0.027145881969705195	0.0
23	0.0	0.0	0.0	0.027921478597411058	0.0
24	0.0	0.0	0.0	0.03645304150217555	0.0
25	0.0	0.0	0.0	0.038004234757587275	0.0
26	0.0	0.0	0.0	0.039555428012999	0.0
27	0.0	0.0	0.0	0.05661855382252798	0.0
28	0.0	0.0	0.0	0.07213048637664524	0.0
29	0.0	0.0	0.0	0.09152040206929181	0.0
30	0.0	0.0	0.0	0.11478830090046768	0.0
31	0.0	0.0	0.0	0.1372806031039377	0.0
32	0.0	0.0	0.0	0.16752887158446636	0.0
33	0.0	0.0	0.0	0.20553310634205363	0.0
34	0.0	0.0	0.0	0.2520689040044054	0.0
35	0.0	0.0	0.0	0.3412625161905796	0.0
36	0.0	0.0	0.0	0.4785431192945173	0.0
37	0.0	0.0	0.0	0.7577579052686279	0.0
38	0.0	0.0	0.0	1.0633429765847378	0.0
39	0.0	0.0	0.0	1.4557948702039043	0.0
40	0.0	0.0	0.0	1.9102944940395399	0.0
41	0.0	0.0	0.0	2.429168637974762	0.0
42	0.0	0.0	0.0	3.062831082810452	0.0
43	0.0	0.0	0.0	3.67245003218726	0.0
44	0.0	0.0	0.0	4.323175602832479	0.0
45	0.0	0.0	0.0	4.994842282425756	0.0
46	0.0	0.0	0.0	5.688225667594797	0.0
47	0.0	0.0	0.0	6.412632917872073	0.0
48	0.0	0.0	0.0	7.03931499305841	0.0
49	0.0	0.0	0.0	7.615583287443866	0.0
50	0.0	0.0	0.0	8.237611782863969	0.0
51	0.0	0.0	0.0	8.917034428734304	0.0
52	0.0	0.0	0.0	9.580169545422816	0.0
53	0.0	0.0	0.0	10.16807178922386	0.0
54	0.0	0.0	0.0	10.844392048583373	0.0
55	0.0	0.0	0.0	11.511405148410415	0.0
56	0.0	0.0	0.0	12.081468669774225	0.0
57	0.0	0.0	0.0	12.672473300086091	0.0
58	0.0	0.0	0.0	13.216942132735607	0.0
59	0.0	0.0	0.0	13.755981788991182	0.0
60	0.0	0.0	0.0	14.340006049653697	0.0
61	0.0	0.0	0.0	14.844143857662507	0.0
62	0.0	0.0	0.0	15.379305530779552	0.0
63	0.0	0.0	0.0	15.914467203896598	0.0
64	0.0	0.0	0.0	16.38447876028635	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTTG	15	0.0022207554	69.997284	11
TAGATCC	15	0.0022207554	69.997284	39
GGTGGAC	35	2.9559305E-7	59.99767	47
GTGGACG	25	2.3835104E-4	55.99783	48
TGTACGG	25	2.3835104E-4	55.99783	54
ATTTGAC	25	2.3835104E-4	55.99783	35
AATGTGC	25	2.3835104E-4	55.99783	45
GCCGTAT	45	2.6793714E-8	54.442333	54
TGGACGC	20	0.006939176	52.497967	49
TGACCAG	20	0.006939176	52.497967	16
CTCGGCG	20	0.006939176	52.497967	44
ACGTGTA	20	0.006939176	52.497967	34
TCCGTAT	20	0.006939176	52.497967	13
GCGTAGA	20	0.006939176	52.497967	36
TGCGTTG	20	0.006939176	52.497967	30
GTAGATC	125	0.0	50.39805	38
TCGGCGG	35	2.077555E-5	49.99806	45
GGCGGTC	35	2.077555E-5	49.99806	47
CCGACAA	35	2.077555E-5	49.99806	21
ATCTCGG	85	0.0	49.409847	42
>>END_MODULE