FastQCFastQC Report
Wed 25 May 2016
SRR1779783_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779783_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128933
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT103418.020444727106327No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG14641.135473462961383No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC6730.5219765304460456No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT4280.33195535665810927RNA PCR Primer, Index 30 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC3160.24508853435505265No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT2470.1915723670433481No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT2440.1892455771602305No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT1450.1124615110173501No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGACC150.00221826570.01707555
ATCGTAT353.6834535E-969.9899239
CTTCGTC150.002221682669.98991421
ATAGAGC150.002221682669.9899148
GGATATC207.907626E-569.98991435
CGGATAT207.907626E-569.98991434
ATGACGG150.002221682669.98991444
CGTACAG150.002221682669.98991433
GACGACG150.002221682669.9899144
CTGGATC150.002221682669.98991445
CGTCTTA308.326071E-658.34756548
TATCGTA252.3847507E-455.99193238
AGTACCG252.3847507E-455.9919325
CTTTATA452.6815542E-854.43662
CGGAAAA200.00693141952.51280253
GGTAACG200.00693141952.51280252
GGGATAT200.00693141952.51280251
GGTCGGA200.006942067852.4924325
GCTGGAC200.006942067852.492433
TCTTCGT200.006942067852.4924320