##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779783_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	128933
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.38412974180388	34.0	34.0	34.0	34.0	34.0
2	33.55011517609921	34.0	34.0	34.0	34.0	34.0
3	33.68909433581783	34.0	34.0	34.0	34.0	34.0
4	33.425088999713026	34.0	34.0	34.0	34.0	34.0
5	33.50340874717877	34.0	34.0	34.0	34.0	34.0
6	37.092645017179464	38.0	37.0	38.0	36.0	38.0
7	37.194899676576206	38.0	38.0	38.0	36.0	38.0
8	37.035910123862784	38.0	37.0	38.0	36.0	38.0
9	36.93573406342829	38.0	37.0	38.0	35.0	38.0
10-11	36.87300380817945	38.0	37.0	38.0	35.0	38.0
12-13	37.076245026486625	38.0	37.5	38.0	35.5	38.0
14-15	37.09770578517525	38.0	38.0	38.0	36.0	38.0
16-17	36.99732806961755	38.0	37.5	38.0	35.0	38.0
18-19	36.86298697773262	38.0	37.0	38.0	34.5	38.0
20-21	36.892510063366245	38.0	37.0	38.0	34.5	38.0
22-23	36.824346753740315	38.0	37.0	38.0	34.5	38.0
24-25	37.06724810560523	38.0	37.5	38.0	36.0	38.0
26-27	36.92826894588663	38.0	38.0	38.0	35.0	38.0
28-29	36.318382415673256	38.0	37.0	38.0	34.0	38.0
30-31	36.783883877672906	38.0	37.0	38.0	34.5	38.0
32-33	36.76202368672101	38.0	37.0	38.0	34.5	38.0
34-35	36.877064056525484	38.0	37.5	38.0	35.5	38.0
36-37	36.79050747287351	38.0	37.0	38.0	34.5	38.0
38-39	35.9835534735095	38.0	37.0	38.0	31.5	38.0
40-41	36.489366570234154	38.0	37.0	38.0	34.0	38.0
42-43	36.914827856328486	38.0	37.0	38.0	35.5	38.0
44-45	36.621648453072524	38.0	37.0	38.0	34.0	38.0
46-47	36.81357371658148	38.0	37.0	38.0	35.0	38.0
48-49	36.38004234757587	38.0	37.0	38.0	34.0	38.0
50-51	36.57038539396431	38.0	37.0	38.0	34.0	38.0
52-53	36.488691801168045	38.0	37.0	38.0	34.0	38.0
54-55	36.501144005025864	38.0	37.0	38.0	34.0	38.0
56-57	36.34113066476387	38.0	37.0	38.0	34.0	38.0
58-59	36.493698277399886	38.0	37.0	38.0	34.0	38.0
60-61	36.06426205858857	38.0	37.0	38.0	33.5	38.0
62-63	35.857976623517644	38.0	37.0	38.0	32.0	38.0
64-65	36.29421870273708	38.0	37.0	38.0	34.0	38.0
66-67	36.08469515174548	38.0	37.0	38.0	32.5	38.0
68-69	36.17435412190828	38.0	37.0	38.0	34.0	38.0
70-71	35.51441446332591	38.0	36.5	38.0	30.0	38.0
72-73	34.28949144129122	38.0	36.0	38.0	22.5	38.0
74-75	34.13124646134038	38.0	36.0	38.0	21.5	38.0
76	33.7815687217392	38.0	34.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	4.0
22	4.0
23	11.0
24	39.0
25	80.0
26	165.0
27	290.0
28	561.0
29	901.0
30	1483.0
31	2401.0
32	3627.0
33	5786.0
34	9334.0
35	16069.0
36	22108.0
37	66069.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.45999130920603	18.99171270718232	12.027438078092992	33.52085790551865
2	17.271761302381858	18.174555777031483	41.36411934880907	23.18956357177759
3	16.98091256699216	21.288576237270522	37.355835976825176	24.37467521891215
4	12.654634577648856	25.07348778047513	35.39202531547393	26.879852326402087
5	11.99227505758805	33.219579161269806	42.05052236432876	12.737623416813385
6	25.710252611821645	43.75450815539854	17.58975591974126	12.945483313038556
7	23.975242955643626	28.41088006949346	21.394832975266223	26.219043999596693
8	21.280820270993463	42.17616901801711	17.84802959676731	18.694981114222113
9	21.486353377335515	15.747713928939838	19.382159726369512	43.38377296735514
10-11	22.569474067926755	28.468662018257547	28.402736304902547	20.559127608913155
12-13	22.244499080917997	20.95351849410159	29.16786237813438	27.63412004684604
14-15	20.364452855358987	21.756648802091007	21.968774479768562	35.91012386278145
16-17	25.99955015395593	29.013906447534765	22.0796847975305	22.9068586009788
18-19	26.01894006964858	24.91216368191231	23.551379398602375	25.517516849836735
20-21	20.434644350166366	24.107094382353626	22.982479272180125	32.47578199529988
22-23	20.853854327441386	29.120550983844325	23.396260073061203	26.62933461565309
24-25	25.950501039307543	29.094018552415225	23.217277324481124	21.738203083796108
26-27	25.656736444509935	30.011323710764504	24.011695997145804	20.32024384757975
28-29	20.478465559631747	29.561477666695108	24.570901165721732	25.38915560795142
30-31	20.134876253558048	24.5802083252542	34.39150566573336	20.893409755454385
32-33	25.605547067081353	23.99657186290554	25.246058030139686	25.15182303987342
34-35	25.992181985992723	23.430774122994112	23.961670014658775	26.615373876354386
36-37	27.100509566984403	28.121970325673022	23.683618623626224	21.093901483716348
38-39	20.542840079731334	25.495412345947123	28.670317141461073	25.29143043286048
40-41	19.807574476666176	22.898327038074036	30.581775030442166	26.712323454817614
42-43	25.48232602330677	22.851879859616467	29.91487794000737	21.750916177069396
44-45	19.919648189369674	28.171608509846198	30.93971287412842	20.969030426655706
46-47	24.885405598256458	22.957272381779685	26.38773626612271	25.76958575384114
48-49	24.40686247896194	22.350368020599845	25.489595371239325	27.75317412919889
50-51	20.167089432407902	22.600901383103334	30.800229612219095	26.43177957226967
52-53	18.621304988750097	22.575451935759176	36.436108309411125	22.3671347660796
54-55	17.96740113060111	22.973628360473192	31.93307958826565	27.125890920660044
56-57	23.711360206104768	22.86009164522975	26.250033949862843	27.178514198802638
58-59	18.382198912259195	23.14785361274255	35.10757151391486	23.362375961083394
60-61	23.36169073748647	28.213095612166917	26.342515546141883	22.08269810420473
62-63	17.925871331872223	35.267169650303536	24.058263348784887	22.748695669039353
64-65	17.915223537898857	35.96852201635956	23.25050129736145	22.86575314838014
66-67	17.776010551633174	36.129257506400805	22.845837535883312	23.248894406082705
68-69	17.805977211203587	36.5039597278996	22.522358306896365	23.16770475400045
70-71	18.260373802053234	34.85419082598696	22.8291517509124	24.056283621047406
72-73	18.845998696744964	32.25323486517516	22.777872591305425	26.122893846774442
74-75	19.281486825060696	31.08812510180811	23.125373296410924	26.505014776720266
76	19.56918017018702	30.730625130898176	23.487980638705526	26.212214060209284
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	3.5
15	6.0
16	8.0
17	11.0
18	19.5
19	39.5
20	63.5
21	76.0
22	98.5
23	155.0
24	230.0
25	271.0
26	348.5
27	465.0
28	647.5
29	791.0
30	917.5
31	1170.0
32	1606.0
33	1916.0
34	2120.0
35	2645.5
36	3389.5
37	3812.0
38	3961.0
39	4882.5
40	6092.0
41	7980.0
42	9431.0
43	10501.0
44	11789.5
45	11014.0
46	10020.0
47	9387.0
48	8000.5
49	6862.0
50	6477.0
51	5890.0
52	4846.0
53	4113.0
54	3837.0
55	3534.0
56	2958.0
57	2275.5
58	1866.0
59	1722.0
60	1418.5
61	1149.0
62	1039.0
63	899.5
64	761.5
65	690.5
66	592.5
67	567.0
68	530.5
69	516.5
70	477.5
71	416.0
72	386.5
73	330.0
74	272.0
75	241.0
76	238.5
77	227.0
78	185.0
79	152.0
80	129.5
81	103.5
82	100.5
83	101.0
84	70.5
85	32.0
86	25.5
87	27.0
88	22.5
89	15.0
90	8.0
91	4.0
92	4.0
93	3.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04731139429005762
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	7.755966277058628E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	3.877983138529314E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.015511932554117255
52-53	0.0333506549913521
54-55	0.04925038585932228
56-57	0.051577175742439876
58-59	0.032575058363646235
60-61	0.03296285667749917
62-63	0.027533680283558126
64-65	0.013572940984852597
66-67	0.0333506549913521
68-69	0.007755966277058627
70-71	0.011246151101735009
72-73	0.019389915692646566
74-75	0.010858352787882079
76	0.012409546043293805
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	128933.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.07040090589686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	47.01947079380929	14.609138079467632
2	18.050424363454816	11.216678429882187
3	11.979530703944084	11.166264649081306
4	7.641038442336495	9.496405109630583
5	4.760359460808787	7.3953138451754015
6	2.698452321517723	5.030519727300225
7	1.7873190214677985	3.887290298061784
8	1.1208187718422367	2.785943086719459
9	0.8212680978532202	2.2965416146370594
>10	4.051422865701448	20.272544654975842
>50	0.042436345481777334	0.8244592152513321
>100	0.019970044932601096	1.3410065693034368
>500	0.002496255616575137	0.5219765304460456
>1k	0.002496255616575137	1.135473462961383
>5k	0.0	0.0
>10k+	0.002496255616575137	8.020444727106327
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT	10341	8.020444727106327	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	1464	1.135473462961383	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	673	0.5219765304460456	No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT	428	0.33195535665810927	RNA PCR Primer, Index 30 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	316	0.24508853435505265	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT	247	0.1915723670433481	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT	244	0.1892455771602305	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT	145	0.1124615110173501	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.755966277058627E-4	0.0
2	0.0	0.0	0.0	7.755966277058627E-4	0.0
3	0.0	0.0	0.0	7.755966277058627E-4	0.0
4	0.0	0.0	0.0	0.0015511932554117254	0.0
5	0.0	0.0	0.0	0.0031023865108234508	0.0
6	0.0	0.0	0.0	0.0031023865108234508	0.0
7	0.0	0.0	0.0	0.0031023865108234508	0.0
8	0.0	0.0	0.0	0.0031023865108234508	0.0
9	0.0	0.0	0.0	0.0062047730216469015	0.0
10	0.0	0.0	0.0	0.007755966277058627	0.0
11	0.0	0.0	0.0	0.007755966277058627	0.0
12	0.0	0.0	0.0	0.010082756160176216	0.0
13	0.0	0.0	0.0	0.010082756160176216	0.0
14	0.0	0.0	0.0	0.010858352787882079	0.0
15	0.0	0.0	0.0	0.012409546043293803	0.0
16	0.0	0.0	0.0	0.013185142670999666	0.0
17	0.0	0.0	0.0	0.013960739298705529	0.0
18	0.0	0.0	0.0	0.017838722437234843	0.0
19	0.0	0.0	0.0	0.020165512320352432	0.0
20	0.0	0.0	0.0	0.020165512320352432	0.0
21	0.0	0.0	0.0	0.021716705575764158	0.0
22	0.0	0.0	0.0	0.024043495458881743	0.0
23	0.0	0.0	0.0	0.02869707522511692	0.0
24	0.0	0.0	0.0	0.030248268480528646	0.0
25	0.0	0.0	0.0	0.0333506549913521	0.0
26	0.0	0.0	0.0	0.034901848246763824	0.0
27	0.0	0.0	0.0	0.0519649740562928	0.0
28	0.0	0.0	0.0	0.06437452009958661	0.0
29	0.0	0.0	0.0	0.0853156290476449	0.0
30	0.0	0.0	0.0	0.10703233462340905	0.0
31	0.0	0.0	0.0	0.12874904019917321	0.0
32	0.0	0.0	0.0	0.16365088844593703	0.0
33	0.0	0.0	0.0	0.20320631645893603	0.0
34	0.0	0.0	0.0	0.2512933073766995	0.0
35	0.0	0.0	0.0	0.3459160959568148	0.0
36	0.0	0.0	0.0	0.5018110181256932	0.0
37	0.0	0.0	0.0	0.8058448961863914	0.0
38	0.0	0.0	0.0	1.1145323540133247	0.0
39	0.0	0.0	0.0	1.5116378273987265	0.0
40	0.0	0.0	0.0	1.972342224256009	0.0
41	0.0	0.0	0.0	2.509055090628466	0.0
42	0.0	0.0	0.0	3.152800291624332	0.0
43	0.0	0.0	0.0	3.781809156693787	0.0
44	0.0	0.0	0.0	4.448822256520828	0.0
45	0.0	0.0	0.0	5.126693709135752	0.0
46	0.0	0.0	0.0	5.8464473796467935	0.0
47	0.0	0.0	0.0	6.579386192828833	0.0
48	0.0	0.0	0.0	7.214599830919935	0.0
49	0.0	0.0	0.0	7.802502074720979	0.0
50	0.0	0.0	0.0	8.44702287234455	0.0
51	0.0	0.0	0.0	9.117138358682416	0.0
52	0.0	0.0	0.0	9.774844298976989	0.0
53	0.0	0.0	0.0	10.34801020685162	0.0
54	0.0	0.0	0.0	11.011920920167839	0.0
55	0.0	0.0	0.0	11.664973280696175	0.0
56	0.0	0.0	0.0	12.22650523915522	0.0
57	0.0	0.0	0.0	12.813631886328558	0.0
58	0.0	0.0	0.0	13.340261996540839	0.0
59	0.0	0.0	0.0	13.879301652796414	0.0
60	0.0	0.0	0.0	14.457121140437282	0.0
61	0.0	0.0	0.0	14.962034545073799	0.0
62	0.0	0.0	0.0	15.497971814818548	0.0
63	0.0	0.0	0.0	16.016070362126065	0.0
64	0.0	0.0	0.0	16.485306321888114	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGACC	15	0.002218265	70.017075	55
ATCGTAT	35	3.6834535E-9	69.98992	39
CTTCGTC	15	0.0022216826	69.989914	21
ATAGAGC	15	0.0022216826	69.989914	8
GGATATC	20	7.907626E-5	69.989914	35
CGGATAT	20	7.907626E-5	69.989914	34
ATGACGG	15	0.0022216826	69.989914	44
CGTACAG	15	0.0022216826	69.989914	33
GACGACG	15	0.0022216826	69.989914	4
CTGGATC	15	0.0022216826	69.989914	45
CGTCTTA	30	8.326071E-6	58.347565	48
TATCGTA	25	2.3847507E-4	55.991932	38
AGTACCG	25	2.3847507E-4	55.991932	5
CTTTATA	45	2.6815542E-8	54.4366	2
CGGAAAA	20	0.006931419	52.512802	53
GGTAACG	20	0.006931419	52.512802	52
GGGATAT	20	0.006931419	52.512802	51
GGTCGGA	20	0.0069420678	52.49243	25
GCTGGAC	20	0.0069420678	52.49243	3
TCTTCGT	20	0.0069420678	52.49243	20
>>END_MODULE