FastQCFastQC Report
Wed 25 May 2016
SRR1779782_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779782_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133005
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGT150.002220569270.034
ACATTGA150.002220569270.028
CATCGTT150.002220569270.032
ATGCGTG150.002220569270.07
TCGCCGG252.3833304E-456.00000452
CGGTAAG252.3833304E-456.00000428
ATCTCGG452.6793714E-854.44444342
CATCTTA200.006938596352.59
AAACGAC200.006938596352.51
TTCCACG200.006938596352.534
GTGTAAT200.006938596352.58
CGGACTT200.006938596352.539
CGGTGGT506.1460014E-849.00000446
GTAGATC651.8371793E-1048.4615438
TCGTTCG305.8605673E-446.66666423
GCCGGAT305.8605673E-446.66666454
TTCGGTA305.8605673E-446.66666426
CCGAAGA305.8605673E-446.66666421
GATCTCG451.6744889E-646.66666441
GTAAGCA404.5728186E-543.7530
Produced by FastQC (version 0.11.5)