##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779782_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	133005
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.506326829818427	34.0	27.0	34.0	12.0	34.0
2	30.072079996992596	34.0	31.0	34.0	12.0	34.0
3	29.702101424758467	34.0	31.0	34.0	11.0	34.0
4	29.93600240592459	34.0	31.0	34.0	12.0	34.0
5	30.42150295101688	34.0	32.0	34.0	11.0	34.0
6	33.19354911469494	38.0	34.0	38.0	12.0	38.0
7	33.071816848990636	38.0	34.0	38.0	12.0	38.0
8	32.50654486673433	37.0	32.0	38.0	11.0	38.0
9	33.403721664599075	38.0	34.0	38.0	12.0	38.0
10-11	32.108574865606556	37.0	30.0	38.0	11.5	38.0
12-13	32.9536671553701	38.0	34.0	38.0	11.0	38.0
14-15	31.697278297808353	37.0	28.5	38.0	11.0	38.0
16-17	32.34606969662795	37.5	30.5	38.0	11.0	38.0
18-19	33.140246607270406	38.0	34.0	38.0	11.0	38.0
20-21	31.932517574527274	37.0	29.5	38.0	11.0	38.0
22-23	32.80435321980377	38.0	32.5	38.0	11.0	38.0
24-25	33.12566444870494	38.0	34.0	38.0	11.0	38.0
26-27	32.03255140784181	37.5	29.0	38.0	11.0	38.0
28-29	33.19086124581783	38.0	34.0	38.0	11.0	38.0
30-31	33.522499154167136	38.0	35.0	38.0	11.0	38.0
32-33	33.60582308935754	38.0	35.0	38.0	11.0	38.0
34-35	33.604105108830495	38.0	34.5	38.0	11.0	38.0
36-37	33.118082026991466	38.0	34.0	38.0	11.0	38.0
38-39	33.09443629938724	38.0	34.0	38.0	11.0	38.0
40-41	33.13746851622119	38.0	34.0	38.0	11.0	38.0
42-43	33.44759971429646	38.0	35.0	38.0	11.0	38.0
44-45	33.52465320852599	38.0	34.5	38.0	11.0	38.0
46-47	33.446731325890006	38.0	34.0	38.0	11.0	38.0
48-49	33.20710499605278	38.0	34.0	38.0	11.0	38.0
50-51	32.59772940866885	38.0	32.5	38.0	11.0	38.0
52-53	33.19471448441788	38.0	34.0	38.0	11.0	38.0
54-55	33.29949250028194	38.0	34.0	38.0	11.0	38.0
56-57	33.311375512198794	38.0	34.0	38.0	11.0	38.0
58-59	33.49492876207661	38.0	35.0	38.0	11.0	38.0
60-61	33.42738242923198	38.0	34.0	38.0	11.0	38.0
62-63	33.418965452426605	38.0	34.0	38.0	11.0	38.0
64-65	33.4373895718206	38.0	34.5	38.0	11.0	38.0
66-67	33.28239915792639	38.0	34.0	38.0	11.0	38.0
68-69	32.70793955114469	38.0	32.5	38.0	11.0	38.0
70-71	32.72031878500808	37.5	32.0	38.0	11.0	38.0
72-73	32.935942257809856	38.0	33.5	38.0	11.0	38.0
74-75	33.05356189616931	38.0	33.5	38.0	11.0	38.0
76	32.153610766512536	37.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	22.0
13	342.0
14	1324.0
15	2384.0
16	3298.0
17	3574.0
18	3400.0
19	2864.0
20	2087.0
21	1429.0
22	1013.0
23	811.0
24	692.0
25	734.0
26	776.0
27	951.0
28	1126.0
29	1480.0
30	1967.0
31	2540.0
32	3464.0
33	4928.0
34	7108.0
35	11514.0
36	22372.0
37	50802.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.23193287419927	23.738383808005775	9.583170430964483	28.446512886830472
2	19.71354460358633	27.25987744821623	29.54550580805233	23.481072140145105
3	18.888763580316528	23.43370549979324	31.977745197548963	25.699785722341268
4	13.228825983985566	22.32472463441224	31.917597082816435	32.52885229878576
5	13.186722303672793	34.102477350475546	35.17311379271456	17.5376865531371
6	24.79323930107365	41.16868665604042	17.478421701602958	16.55965234128297
7	25.029698801521782	29.04993909866017	20.770364355423226	25.14999774439482
8	22.694566206269126	35.79973082909151	20.213381854271773	21.292321110367592
9	23.266690226095328	19.455326059986316	19.999699241336273	37.278284472582094
10-11	22.841557582273815	26.819347222953212	24.7385358004827	25.60055939429027
12-13	22.467952332619074	23.79985714822751	26.36367053870155	27.36851998045186
14-15	24.027472857959157	25.376680400589457	22.89254458512526	27.703302156326124
16-17	25.14238240059547	26.369032626715637	23.169516820859446	25.31906815182945
18-19	22.209064525345486	26.17366656140509	25.39247530112329	26.224793612126135
20-21	23.554001729258296	25.856922672079996	25.18815082139769	25.40092477726401
22-23	24.94502065719581	25.638982139702037	24.426617144532702	24.989380058569445
24-25	21.685388189651285	28.211981774710154	24.17332070194433	25.929309333694228
26-27	23.02007090038984	28.60300668779393	24.499355280124206	23.877567131692025
28-29	22.20158722401212	27.51250944552423	26.236564799115786	24.049338531347864
30-31	22.686450458443137	27.18178708239195	25.820930870760012	24.310831588404906
32-33	21.021093271280293	28.449037438582902	25.900627422380445	24.629241867756356
34-35	21.44951617632685	28.95047480132027	25.527619677751627	24.07238934460125
36-37	23.536622048428335	26.6449842081516	25.093623101218228	24.724770642201836
38-39	22.986751282012722	25.95681008165782	26.650450396258478	24.40598824007098
40-41	21.82069027739663	26.83518226826912	26.39234010879331	24.95178734554094
42-43	20.404196850482503	26.687442906067087	27.04006255427448	25.86829768917593
44-45	19.870151352245472	25.615220938188433	28.31256907842798	26.202058631138115
46-47	20.709087081397275	26.40787356581104	27.72740259544969	25.15563675734199
48-49	20.750801664605333	26.787439522722917	27.3114819423403	25.150276870331457
50-51	22.748649471630447	26.39141075234862	25.725638798978974	25.134300977041956
52-53	18.840912081508357	27.393274056807716	27.403049044119026	26.362764817564898
54-55	19.12359584354652	26.41092347253342	27.25943247266876	27.2060482112513
56-57	19.794498144618263	26.912998199133025	26.81299180774711	26.4795118485016
58-59	19.626656640661714	26.99050662656265	26.612275589811073	26.770561142964567
60-61	19.164015912047585	26.36842857250284	26.61583233695039	27.85172317849918
62-63	19.34650695901288	26.430714398502175	26.644259472303055	27.578519170181888
64-65	20.26515863645593	25.956744399407416	25.786414191927925	27.991682772208726
66-67	19.546098864119173	27.969890322265297	24.745733397001814	27.738277416613716
68-69	19.69805192364082	28.688450636451808	24.302835274392308	27.310662165515065
70-71	20.656599262425424	27.960542391741573	23.910648967884303	27.472209377948705
72-73	21.245112781954887	27.314661654135335	23.70827067669173	27.731954887218045
74-75	20.60117302052786	26.92796450860967	24.270621851267013	28.20024061959546
76	20.58224250580814	26.97457952076269	24.227273069028517	28.215904904400652
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.5
11	0.5
12	0.0
13	0.0
14	0.5
15	5.0
16	9.0
17	9.0
18	18.5
19	21.0
20	29.5
21	45.0
22	41.0
23	58.0
24	118.0
25	157.0
26	214.0
27	336.0
28	481.5
29	562.0
30	650.5
31	916.5
32	1368.0
33	1642.0
34	1918.0
35	2591.0
36	3398.5
37	3809.0
38	4294.5
39	5375.5
40	6448.0
41	7233.0
42	7541.0
43	7915.0
44	8656.5
45	9087.0
46	9150.0
47	9148.0
48	8862.0
49	8349.5
50	8121.0
51	7590.0
52	6465.5
53	5547.5
54	5223.0
55	4801.0
56	3880.0
57	3127.5
58	2874.0
59	2516.0
60	1950.5
61	1554.5
62	1366.0
63	1247.5
64	1040.0
65	862.5
66	715.0
67	656.0
68	613.0
69	525.5
70	461.5
71	442.0
72	425.5
73	371.0
74	303.5
75	274.0
76	259.5
77	234.0
78	189.0
79	155.0
80	149.5
81	138.0
82	116.0
83	100.0
84	95.5
85	81.0
86	53.0
87	35.0
88	35.0
89	33.0
90	22.0
91	13.0
92	13.0
93	11.5
94	10.0
95	7.5
96	5.0
97	4.0
98	3.0
99	4.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.518514341566106E-4
2	0.0
3	0.0
4	0.0
5	0.0
6	7.518514341566106E-4
7	0.002255554302469832
8	0.0030074057366264425
9	0.006014811473252885
10-11	0.0030074057366264425
12-13	0.0
14-15	7.518514341566106E-4
16-17	3.759257170783053E-4
18-19	0.002255554302469832
20-21	0.0
22-23	3.759257170783053E-4
24-25	0.002255554302469832
26-27	0.001127777151234916
28-29	0.0041351828878613585
30-31	3.759257170783053E-4
32-33	3.759257170783053E-4
34-35	0.0015037028683132212
36-37	0.018796285853915264
38-39	0.008270365775722717
40-41	3.759257170783053E-4
42-43	3.759257170783053E-4
44-45	0.0030074057366264425
46-47	0.002255554302469832
48-49	3.759257170783053E-4
50-51	0.001127777151234916
52-53	0.009398142926957632
54-55	0.0052629600390962745
56-57	0.010149994361114243
58-59	0.013157400097740687
60-61	0.018044434419758656
62-63	0.009022217209879328
64-65	0.021051840156385098
66-67	0.018420360136836962
68-69	0.0015037028683132212
70-71	0.001127777151234916
72-73	0.0037592571707830533
74-75	0.01127777151234916
76	0.0015037028683132212
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	133005.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.49994361114244
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7941025452945	48.458328634261875
2	8.723929680432283	10.730423668283146
3	4.597911929387027	8.483139731589038
4	2.7201154062446515	6.691477763993834
5	1.6699674808675027	5.135145295289651
6	0.982909117582337	3.6269313183714895
7	0.6173745079341794	2.6577948197436188
8	0.4058778943250446	1.9969174091199577
9	0.3251913249712707	1.7999323333709258
>10	1.1613975891831096	10.3725423856246
>50	0.0012225237780874839	0.04736664035186647
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0037592571707830533	0.0
5	0.0	0.0	0.0	0.0037592571707830533	0.0
6	0.0	0.0	0.0	0.0037592571707830533	0.0
7	0.0	0.0	0.0	0.006014811473252885	0.0
8	0.0	0.0	0.0	0.009022217209879328	0.0
9	0.0	0.0	0.0	0.01127777151234916	0.0
10	0.0	0.0	0.0	0.01127777151234916	0.0
11	0.0	0.0	0.0	0.01127777151234916	0.0
12	0.0	0.002255554302469832	0.0	0.01127777151234916	0.0
13	0.0	0.002255554302469832	0.0	0.01127777151234916	0.0
14	0.0	0.002255554302469832	0.0	0.01202962294650577	0.0
15	0.0	0.002255554302469832	0.0	0.015037028683132213	0.0
16	0.0	0.002255554302469832	0.0	0.015037028683132213	0.0
17	0.0	0.002255554302469832	0.0	0.015037028683132213	0.0
18	0.0	0.002255554302469832	0.0	0.015037028683132213	0.0
19	0.0	0.002255554302469832	0.0	0.021051840156385098	0.0
20	0.0	0.002255554302469832	0.0	0.025562948761324762	0.0
21	0.0	0.002255554302469832	0.0	0.026314800195481373	0.0
22	0.0	0.002255554302469832	0.0	0.03608886883951731	0.0
23	0.0	0.002255554302469832	0.0	0.041351828878613585	0.0
24	0.0	0.002255554302469832	0.0	0.05262960039096275	0.0
25	0.0	0.002255554302469832	0.0	0.05789256043005902	0.0
26	0.0	0.002255554302469832	0.0	0.062403669034998686	0.0
27	0.0	0.002255554302469832	0.0	0.07443329198150446	0.0
28	0.0	0.002255554302469832	0.0	0.08270365775722717	0.0
29	0.0	0.002255554302469832	0.0	0.10450734934776888	0.0
30	0.0	0.002255554302469832	0.0	0.15037028683132214	0.0
31	0.0	0.002255554302469832	0.0	0.19698507574903198	0.0
32	0.0	0.002255554302469832	0.0	0.23532949889101915	0.0
33	0.0	0.002255554302469832	0.0	0.29773316792601784	0.0
34	0.0	0.002255554302469832	0.0	0.3601368369610165	0.0
35	0.0	0.002255554302469832	0.0	0.4646441863087854	0.0
36	0.0	0.002255554302469832	0.0	0.6315552046915529	0.0
37	0.0	0.002255554302469832	0.0	0.8541032292019097	0.0
38	0.0	0.002255554302469832	0.0	1.220254877636179	0.0
39	0.0	0.002255554302469832	0.0	1.7412879215067103	0.0
40	0.0	0.002255554302469832	0.0	2.293898725611819	0.0
41	0.0	0.002255554302469832	0.0	2.946505770459757	0.0
42	0.0	0.002255554302469832	0.0	3.5577609864290816	0.0
43	0.0	0.002255554302469832	0.0	4.193827299725574	0.0
44	0.0	0.002255554302469832	0.0	4.814104732904778	0.0
45	0.0	0.002255554302469832	0.0	5.556182098417353	0.0
46	0.0	0.002255554302469832	0.0	6.259163189353783	0.0
47	0.0	0.002255554302469832	0.0	7.03432201796925	0.0
48	0.0	0.002255554302469832	0.0	7.734295703169054	0.0
49	0.0	0.002255554302469832	0.0	8.480884177286567	0.0
50	0.0	0.002255554302469832	0.0	9.219954137062517	0.0
51	0.0	0.002255554302469832	0.0	10.067290703357017	0.0
52	0.0	0.002255554302469832	0.0	10.871019886470433	0.0
53	0.0	0.002255554302469832	0.0	11.525130634186684	0.0
54	0.0	0.002255554302469832	0.0	12.217585805044923	0.0
55	0.0	0.002255554302469832	0.0	12.878463215668583	0.0
56	0.0	0.002255554302469832	0.0	13.55588135784369	0.0
57	0.0	0.002255554302469832	0.0	14.239314311492048	0.0
58	0.0	0.002255554302469832	0.0	14.920491710837938	0.0
59	0.0	0.002255554302469832	0.0	15.608435773091237	0.0
60	0.0	0.002255554302469832	0.0	16.31367241833014	0.0
61	0.0	0.002255554302469832	0.0	16.986579451900305	0.0
62	0.0	0.002255554302469832	0.0	17.591819856396377	0.0
63	0.0	0.002255554302469832	0.0	18.206082478102328	0.0
64	0.0	0.002255554302469832	0.0	18.84891545430623	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGT	15	0.0022205692	70.0	34
ACATTGA	15	0.0022205692	70.0	28
CATCGTT	15	0.0022205692	70.0	32
ATGCGTG	15	0.0022205692	70.0	7
TCGCCGG	25	2.3833304E-4	56.000004	52
CGGTAAG	25	2.3833304E-4	56.000004	28
ATCTCGG	45	2.6793714E-8	54.444443	42
CATCTTA	20	0.0069385963	52.5	9
AAACGAC	20	0.0069385963	52.5	1
TTCCACG	20	0.0069385963	52.5	34
GTGTAAT	20	0.0069385963	52.5	8
CGGACTT	20	0.0069385963	52.5	39
CGGTGGT	50	6.1460014E-8	49.000004	46
GTAGATC	65	1.8371793E-10	48.46154	38
TCGTTCG	30	5.8605673E-4	46.666664	23
GCCGGAT	30	5.8605673E-4	46.666664	54
TTCGGTA	30	5.8605673E-4	46.666664	26
CCGAAGA	30	5.8605673E-4	46.666664	21
GATCTCG	45	1.6744889E-6	46.666664	41
GTAAGCA	40	4.5728186E-5	43.75	30
>>END_MODULE