FastQCFastQC Report
Wed 25 May 2016
SRR1779782_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779782_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133005
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT1405810.569527461373633No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG20241.52174730273298No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC10220.7683921657080561No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT6300.4736664035186647Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC4960.3729183113416789No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC2300.17292582985602045Illumina PCR Primer Index 10 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC1970.1481147325288523Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT1870.1405962181872862No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGT1760.13232585241156347No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG1460.10977030938686516No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA150.002218061870.0199348
GTGTACG150.002221373669.9936169
TGTACGC150.002221373669.9936170
TGTGTAC150.002221373669.9936168
CTGGAAG150.002221373669.9936168
ATCGTAT352.9577131E-759.99452239
GCGAAGT252.3844058E-455.9948932
ATATCGT252.3844058E-455.9948937
GAGCATG200.00691017952.5544781
GAACTCC200.006930782552.5149546
TGGCGTC200.006930782552.5149560
CTTATGC200.006930782552.5149551
AATTCAC200.006930782552.5149560
TTTCGTC200.00694110152.4952118
TATATCG200.00694110152.4952136
CCATGAC200.00694110152.495213
GTGTGTA200.00694110152.4952167
TCTAAAA200.00694110152.495212
CTGCATG200.00694110152.4952154
AACGAGA200.00694110152.4952169
Produced by FastQC (version 0.11.5)