##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779782_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	133005
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.4452013082215	34.0	34.0	34.0	34.0	34.0
2	33.58047441825495	34.0	34.0	34.0	34.0	34.0
3	33.72200293222059	34.0	34.0	34.0	34.0	34.0
4	33.4659674448329	34.0	34.0	34.0	34.0	34.0
5	33.520326303522424	34.0	34.0	34.0	34.0	34.0
6	37.15434006240367	38.0	38.0	38.0	36.0	38.0
7	37.251900304499834	38.0	38.0	38.0	36.0	38.0
8	37.1285891507838	38.0	38.0	38.0	36.0	38.0
9	37.0196082854028	38.0	38.0	38.0	36.0	38.0
10-11	36.961362354798695	38.0	37.0	38.0	35.0	38.0
12-13	37.15073869403406	38.0	37.5	38.0	36.0	38.0
14-15	37.17589564302094	38.0	38.0	38.0	36.0	38.0
16-17	37.07436186609526	38.0	38.0	38.0	36.0	38.0
18-19	36.92656291116876	38.0	37.5	38.0	35.0	38.0
20-21	36.954802451035675	38.0	37.0	38.0	35.5	38.0
22-23	36.92030750723657	38.0	37.0	38.0	35.0	38.0
24-25	37.14556219690989	38.0	38.0	38.0	36.0	38.0
26-27	37.02293898725612	38.0	38.0	38.0	36.0	38.0
28-29	36.42711552197286	38.0	37.0	38.0	34.0	38.0
30-31	36.812326604263	38.0	37.0	38.0	35.0	38.0
32-33	36.927262133002515	38.0	37.0	38.0	35.5	38.0
34-35	36.88009097402353	38.0	37.0	38.0	35.5	38.0
36-37	36.84540054885154	38.0	37.0	38.0	35.0	38.0
38-39	36.37899703018684	38.0	37.0	38.0	34.0	38.0
40-41	36.6837487312507	38.0	37.0	38.0	34.5	38.0
42-43	37.02853652118341	38.0	37.5	38.0	36.0	38.0
44-45	36.74301717980527	38.0	37.0	38.0	34.5	38.0
46-47	36.895943761512726	38.0	37.0	38.0	35.5	38.0
48-49	36.48382015713695	38.0	37.0	38.0	34.0	38.0
50-51	36.668425999022595	38.0	37.0	38.0	34.0	38.0
52-53	36.597018909063564	38.0	37.0	38.0	34.0	38.0
54-55	36.59315439269201	38.0	37.0	38.0	34.0	38.0
56-57	36.442772828089176	38.0	37.0	38.0	34.0	38.0
58-59	36.55953911507086	38.0	37.0	38.0	34.0	38.0
60-61	36.15988496673057	38.0	37.0	38.0	34.0	38.0
62-63	35.94438930867261	38.0	37.0	38.0	32.5	38.0
64-65	36.412326604263	38.0	37.0	38.0	34.0	38.0
66-67	36.16441487162137	38.0	37.0	38.0	32.5	38.0
68-69	36.233795721965336	38.0	37.0	38.0	34.0	38.0
70-71	35.3936957257246	38.0	36.5	38.0	28.5	38.0
72-73	33.84732904778016	38.0	35.0	38.0	21.5	38.0
74-75	33.58639524829894	38.0	35.0	38.0	19.0	38.0
76	33.31768730498853	38.0	34.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	3.0
22	0.0
23	16.0
24	30.0
25	51.0
26	161.0
27	286.0
28	469.0
29	806.0
30	1427.0
31	2243.0
32	3575.0
33	5652.0
34	9304.0
35	17082.0
36	23559.0
37	68339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.15261950430629	16.155553048253037	11.016585806160442	34.67524164128023
2	19.625577985790006	18.490282320213524	39.588737265516336	22.29540242848013
3	18.147438066238113	20.269914664862224	38.18878989511673	23.39385737378294
4	12.547648584639676	27.37491071764219	34.497951204841925	25.579489492876206
5	11.972482237509869	31.693545355437763	42.977331679260175	13.356640727792188
6	23.793842336754256	45.04116386602007	18.450434194203226	12.714559603022444
7	23.841208977106124	27.65760685688508	20.73906995977595	27.762114206232845
8	21.508965828352316	41.79767677906845	17.744445697530168	18.94891169504906
9	21.06236607646329	17.83241231532649	18.031652945377992	43.07356866283222
10-11	21.627758354949062	29.179730085335137	28.468102702905906	20.724408856809895
12-13	21.378519604526144	20.653734821999173	29.799255667080182	28.168489906394495
14-15	20.416525694522765	20.336453516785085	21.666854629525208	37.58016615916695
16-17	27.353482951768733	28.5808804180294	21.130408631254465	22.935227998947408
18-19	27.3696477576031	24.27465132889741	22.09428216984324	26.261418743656257
20-21	20.011653697229427	22.887109507161384	22.09691364986279	35.004323145746405
22-23	20.275929476335477	29.49813916770046	22.6754633284463	27.55046802751776
24-25	26.590829633583823	29.620802303681455	22.31954557928491	21.46882248344981
26-27	26.95575354309988	30.96763279575956	22.42434494943799	19.65226871170257
28-29	19.994737039960903	30.476297883538212	23.411149956768543	26.11781511973234
30-31	26.547498214352842	24.60772151422879	28.888011728882372	19.956768542535993
32-33	25.76707642569828	30.244351716100898	24.579151159730834	19.40942069846998
34-35	21.37964738167738	29.92293522799895	29.705650163527686	18.991767226795986
36-37	19.58948911695049	28.966204278034663	31.46573437088831	19.97857223412654
38-39	19.273335588887637	24.082929213187473	30.943949475583626	25.699785722341268
40-41	19.480094733280705	21.889026728318484	31.169880831547687	27.460997706853124
42-43	26.01350335328261	21.7173919581366	30.56562208655379	21.70348260202701
44-45	19.136874553588214	28.551934137814367	31.565730611631142	20.74546069696628
46-47	25.414082177361752	22.273598736889593	25.759933837073795	26.552385248674863
48-49	24.754708469606406	21.313860381188675	24.916732453667155	29.01469869553776
50-51	19.71756850242127	21.23575059403856	31.551342978313833	27.495337925226337
52-53	17.769956229412028	21.319359836349143	38.523005881202714	22.387678053036115
54-55	16.964739069111427	21.748942172073342	33.22689233662435	28.05942642219088
56-57	23.733179893344165	21.72116042752593	26.748576543237736	27.79708313589217
58-59	17.636174917640687	22.129834378807708	37.05567339079682	23.178317312754785
60-61	23.934101968216723	28.60043771575551	25.826357708535458	21.639102607492312
62-63	17.01769229322955	37.288435143926144	23.495591027882753	22.198281534961552
64-65	16.645361165837006	38.366068608725726	22.462514851187343	22.52605537424992
66-67	16.6898564240642	38.23151149585217	22.35016282970194	22.728469250381693
68-69	16.72882439189443	38.38302191811722	22.0910560547389	22.797097635249443
70-71	17.430126850689145	37.15740162868164	21.739441014805514	23.673030505823704
72-73	17.797037148443376	34.32621446834111	21.850278237328922	26.0264701458866
74-75	18.419974208878013	32.94721843125312	22.39879992330337	26.23400743656549
76	18.706107042422968	33.39699535317383	22.182203708437974	25.71469389596523
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	2.0
15	2.5
16	1.5
17	2.0
18	13.0
19	29.5
20	35.5
21	36.0
22	53.0
23	76.0
24	138.0
25	194.0
26	270.0
27	358.5
28	451.0
29	531.0
30	672.0
31	943.0
32	1284.5
33	1496.0
34	1754.0
35	2378.5
36	3144.5
37	3544.0
38	6113.0
39	10499.0
40	12065.0
41	10728.0
42	9642.0
43	8984.5
44	8408.0
45	8203.5
46	7918.0
47	7808.0
48	7468.5
49	6994.5
50	6750.0
51	6208.5
52	5025.0
53	4100.5
54	3818.0
55	3501.0
56	2868.5
57	2197.0
58	1841.0
59	1592.0
60	1238.5
61	1051.0
62	968.0
63	909.5
64	740.5
65	603.5
66	570.5
67	564.0
68	497.5
69	418.5
70	394.5
71	383.0
72	361.0
73	308.5
74	264.0
75	250.0
76	232.5
77	219.5
78	186.0
79	148.0
80	142.5
81	114.5
82	97.0
83	102.0
84	85.0
85	61.5
86	40.5
87	26.0
88	34.5
89	30.5
90	12.5
91	8.0
92	9.0
93	6.5
94	2.0
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04511108604939664
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	3.759257170783053E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	7.518514341566106E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.012781474380662381
52-53	0.029322205932107815
54-55	0.050749971805571226
56-57	0.040599977444456974
58-59	0.03834442314198714
60-61	0.03157776023457765
62-63	0.0281944287808729
64-65	0.014285177248975602
66-67	0.03308146310289087
68-69	0.007518514341566107
70-71	0.010525920078192549
72-73	0.018796285853915264
74-75	0.010149994361114243
76	0.008270365775722717
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	133005.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.524529153039364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	44.524570460815646	12.255178376752754
2	16.594280095058593	9.13499492500282
3	12.1991859925155	10.073305514830269
4	8.118222295064054	8.938009849253788
5	5.424895517495698	7.4658847411751434
6	3.466360730967795	5.724596819668434
7	2.103307929744052	4.052479230104131
8	1.5050943756999644	3.31416112176234
9	1.1336010270698462	2.8081651065749407
>10	4.840339807151247	20.727040336829443
>50	0.06282608101832883	1.0961993910003383
>100	0.016389412439564044	1.0766512537122666
>500	0.00273156873992734	0.4736664035186647
>1k	0.00546313747985468	2.290139468441036
>5k	0.0	0.0
>10k+	0.00273156873992734	10.569527461373633
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT	14058	10.569527461373633	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG	2024	1.52174730273298	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	1022	0.7683921657080561	No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT	630	0.4736664035186647	Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	496	0.3729183113416789	No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC	230	0.17292582985602045	Illumina PCR Primer Index 10 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC	197	0.1481147325288523	Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT	187	0.1405962181872862	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGT	176	0.13232585241156347	No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG	146	0.10977030938686516	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.518514341566106E-4	0.0
2	0.0	0.0	0.0	7.518514341566106E-4	0.0
3	0.0	0.0	0.0	7.518514341566106E-4	0.0
4	0.0	0.0	0.0	0.0052629600390962745	0.0
5	0.0	0.0	0.0	0.0052629600390962745	0.0
6	0.0	0.0	0.0	0.0052629600390962745	0.0
7	0.0	0.0	0.0	0.007518514341566107	0.0
8	0.0	0.0	0.0	0.01127777151234916	0.0
9	0.0	0.0	0.0	0.015788880117288823	0.0
10	0.0	0.0	0.0	0.015788880117288823	0.0
11	0.0	0.0	0.0	0.015788880117288823	0.0
12	0.0	0.0	0.0	0.015788880117288823	0.0
13	0.0	0.0	0.0	0.015788880117288823	0.0
14	0.0	0.0	0.0	0.015788880117288823	0.0
15	0.0	0.0	0.0	0.018796285853915268	0.0
16	0.0	0.0	0.0	0.018796285853915268	0.0
17	0.0	0.0	0.0	0.018796285853915268	0.0
18	0.0	0.0	0.0	0.020299988722228487	0.0
19	0.0	0.0	0.0	0.027818503063794593	0.0
20	0.0	0.0	0.0	0.03383331453704748	0.0
21	0.0	0.0	0.0	0.03458516597120409	0.0
22	0.0	0.0	0.0	0.03984812601030036	0.0
23	0.0	0.0	0.0	0.04285553174692681	0.0
24	0.0	0.0	0.0	0.05037404608849291	0.0
25	0.0	0.0	0.0	0.056388857561745796	0.0
26	0.0	0.0	0.0	0.060899966166685464	0.0
27	0.0	0.0	0.0	0.07593699484981768	0.0
28	0.0	0.0	0.0	0.084959212059697	0.0
29	0.0	0.0	0.0	0.10676290365023872	0.0
30	0.0	0.0	0.0	0.15187398969963536	0.0
31	0.0	0.0	0.0	0.19924063005150183	0.0
32	0.0	0.0	0.0	0.2383369046276456	0.0
33	0.0	0.0	0.0	0.3052516822675839	0.0
34	0.0	0.0	0.0	0.3721664599075223	0.0
35	0.0	0.0	0.0	0.4744182549528213	0.0
36	0.0	0.0	0.0	0.6518551934137814	0.0
37	0.0	0.0	0.0	0.890192098041427	0.0
38	0.0	0.0	0.0	1.2721326265929853	0.0
39	0.0	0.0	0.0	1.7961730762001429	0.0
40	0.0	0.0	0.0	2.3645727604225404	0.0
41	0.0	0.0	0.0	3.0284575767828277	0.0
42	0.0	0.0	0.0	3.659260930040224	0.0
43	0.0	0.0	0.0	4.297582797639187	0.0
44	0.0	0.0	0.0	4.927634299462426	0.0
45	0.0	0.0	0.0	5.668207962106687	0.0
46	0.0	0.0	0.0	6.392240893199504	0.0
47	0.0	0.0	0.0	7.1726626818540655	0.0
48	0.0	0.0	0.0	7.9064696815909175	0.0
49	0.0	0.0	0.0	8.692154430284576	0.0
50	0.0	0.0	0.0	9.451524378782752	0.0
51	0.0	0.0	0.0	10.29510168790647	0.0
52	0.0	0.0	0.0	11.084545693770911	0.0
53	0.0	0.0	0.0	11.728882372843126	0.0
54	0.0	0.0	0.0	12.406300515018232	0.0
55	0.0	0.0	0.0	13.057403856997857	0.0
56	0.0	0.0	0.0	13.729559039133868	0.0
57	0.0	0.0	0.0	14.402466072704033	0.0
58	0.0	0.0	0.0	15.078380512010826	0.0
59	0.0	0.0	0.0	15.768580128566596	0.0
60	0.0	0.0	0.0	16.471561219503027	0.0
61	0.0	0.0	0.0	17.137701590165783	0.0
62	0.0	0.0	0.0	17.73241607458366	0.0
63	0.0	0.0	0.0	18.345926844855455	0.0
64	0.0	0.0	0.0	19.00454870117665	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	15	0.0022180618	70.01993	48
GTGTACG	15	0.0022213736	69.99361	69
TGTACGC	15	0.0022213736	69.99361	70
TGTGTAC	15	0.0022213736	69.99361	68
CTGGAAG	15	0.0022213736	69.99361	68
ATCGTAT	35	2.9577131E-7	59.994522	39
GCGAAGT	25	2.3844058E-4	55.99489	32
ATATCGT	25	2.3844058E-4	55.99489	37
GAGCATG	20	0.006910179	52.554478	1
GAACTCC	20	0.0069307825	52.51495	46
TGGCGTC	20	0.0069307825	52.51495	60
CTTATGC	20	0.0069307825	52.51495	51
AATTCAC	20	0.0069307825	52.51495	60
TTTCGTC	20	0.006941101	52.49521	18
TATATCG	20	0.006941101	52.49521	36
CCATGAC	20	0.006941101	52.49521	3
GTGTGTA	20	0.006941101	52.49521	67
TCTAAAA	20	0.006941101	52.49521	2
CTGCATG	20	0.006941101	52.49521	54
AACGAGA	20	0.006941101	52.49521	69
>>END_MODULE