FastQCFastQC Report
Wed 25 May 2016
SRR1779781_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779781_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37799
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG207.829863E-570.0000145
CTTCCGA150.002208227870.060
TACCTGA252.3614342E-456.0000049
CTTGTCA200.006900326352.5000041
CGGGTGG200.006900326352.50000410
TACCAGG200.006900326352.50000464
GAGTATC200.006900326352.50000428
GCGCCAA200.006900326352.50000447
AAGCCCG200.006900326352.50000415
ACATCCA200.006900326352.50000444
AGGTGTA200.006900326352.50000434
CTAGCAC200.006900326352.5000042
GGATATA305.8070465E-446.6666641
TTCCACC305.8070465E-446.66666446
CGCCGCC305.8070465E-446.66666416
GTGTAGA459.033895E-538.8888936
CCGTATC450.00425638931.11111355
AGAGGTG450.00425638931.11111332
ACGCCGC500.00712375128.00000215
CGACGAA500.00712375128.00000222
Produced by FastQC (version 0.11.5)