##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779781_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37799
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.315299346543558	34.0	21.0	34.0	12.0	34.0
2	27.434270747903383	34.0	21.0	34.0	12.0	34.0
3	27.456467102304295	34.0	23.0	34.0	11.0	34.0
4	27.607449932537897	34.0	23.0	34.0	12.0	34.0
5	27.75573427868462	34.0	23.0	34.0	11.0	34.0
6	30.270721447657344	37.0	24.0	38.0	12.0	38.0
7	30.605836133231037	37.0	26.0	38.0	12.0	38.0
8	30.109844175771848	36.0	24.0	38.0	11.0	38.0
9	30.611391835762852	37.0	26.0	38.0	11.0	38.0
10-11	29.492817270298154	35.0	22.0	38.0	11.0	38.0
12-13	30.06738273499299	37.0	23.0	38.0	11.0	38.0
14-15	28.988862139210035	35.0	21.0	38.0	11.0	38.0
16-17	29.37071086536681	36.0	22.0	38.0	11.0	38.0
18-19	29.818447577978255	37.0	21.5	38.0	11.0	38.0
20-21	29.13950104500119	35.0	22.0	38.0	11.0	38.0
22-23	29.723206963147174	37.0	22.0	38.0	11.0	38.0
24-25	30.02609857403635	37.0	22.5	38.0	11.0	38.0
26-27	29.08861345538242	36.0	21.0	38.0	11.0	38.0
28-29	29.88863461996349	37.0	22.0	38.0	11.0	38.0
30-31	30.133958570332545	37.0	22.0	38.0	11.0	38.0
32-33	30.291515648562132	37.5	23.0	38.0	11.0	38.0
34-35	30.3744543506442	37.0	23.0	38.0	11.0	38.0
36-37	29.96502552977592	37.0	22.0	38.0	11.0	38.0
38-39	29.885711262202705	37.0	21.5	38.0	11.0	38.0
40-41	29.93534220482023	37.0	22.0	38.0	11.0	38.0
42-43	30.203219661895815	37.0	22.5	38.0	11.0	38.0
44-45	30.280179369824598	37.0	23.0	38.0	11.0	38.0
46-47	30.15534802508003	37.0	22.0	38.0	11.0	38.0
48-49	29.965038757639093	37.0	22.0	38.0	11.0	38.0
50-51	29.45018386729808	36.5	21.0	38.0	11.0	38.0
52-53	29.9919310034657	37.0	23.0	38.0	11.0	38.0
54-55	30.082819651313528	37.0	23.0	38.0	11.0	38.0
56-57	30.00578057620572	37.0	22.5	38.0	11.0	38.0
58-59	30.061747665282148	37.0	22.0	38.0	11.0	38.0
60-61	30.10369321939734	37.0	23.0	38.0	11.0	38.0
62-63	30.031244212809863	37.0	22.0	38.0	11.0	38.0
64-65	30.073956982988967	37.0	22.0	38.0	11.0	38.0
66-67	30.079711103468348	37.0	23.0	38.0	11.0	38.0
68-69	29.611087594909918	37.0	21.5	38.0	11.0	38.0
70-71	29.64151168020318	36.5	22.0	38.0	11.0	38.0
72-73	29.612172279689936	37.0	21.5	38.0	11.0	38.0
74-75	29.819558718484615	37.0	22.0	38.0	11.0	38.0
76	29.107225058863992	36.0	21.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	25.0
13	197.0
14	836.0
15	1589.0
16	1955.0
17	2193.0
18	1964.0
19	1444.0
20	1059.0
21	670.0
22	500.0
23	354.0
24	262.0
25	226.0
26	241.0
27	213.0
28	259.0
29	363.0
30	405.0
31	534.0
32	734.0
33	1061.0
34	1575.0
35	2538.0
36	5153.0
37	11449.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.0777269239927	20.931770681764068	10.529379084102754	30.46112331014048
2	16.56657583533956	21.397391465382682	30.540490489166377	31.495542210111378
3	15.455435328976957	22.648747321357707	36.15704119156592	25.73877615809942
4	11.148443080504775	31.495542210111378	28.762665678986217	28.59334903039763
5	11.41564591655864	34.757533268075875	35.998306833514114	17.828513981851373
6	20.475169987036008	38.820541312802604	16.077995608117046	24.626293092044342
7	20.16244245727287	35.528334832530824	18.61474152071538	25.69448118948092
8	17.432139266627864	37.74009206836341	15.966453251494789	28.861315413513942
9	21.925600592655307	19.433273362260557	17.739972483860726	40.90115356122341
10-11	19.162896526179328	28.7668333465619	23.582559462391195	28.48771066486758
12-13	18.98859758194661	24.646154660176194	25.810206619222733	30.55504113865446
14-15	24.929560696853052	26.318504702567562	21.44926385967697	27.302670740902418
16-17	26.4071325581703	25.87139701310899	21.264071325581703	26.45739910313901
18-19	21.783933224863418	25.80922523380557	23.663637445930394	28.74320409540061
20-21	23.492684991666447	25.942485250932563	23.136855472366992	27.427974285033997
22-23	26.40518552814339	25.051921423374562	21.482902308353726	27.059990740128313
24-25	20.680714843181608	30.278979324576373	21.62784237469741	27.41246345754461
26-27	22.841155616699297	30.402402243504945	22.853061008518967	23.903381131276788
28-29	21.90972589692031	28.66044025822838	24.760556672663775	24.669277172187535
30-31	22.343479238594124	27.269600645528264	25.592285408151117	24.794634707726498
32-33	19.233313050609805	29.904230270642078	25.05357284584248	25.808883832905632
34-35	18.925354180721456	31.931161289469156	23.954654285222958	25.188830244586423
36-37	22.9539861879184	27.046013812081604	23.829809753128885	26.170190246871112
38-39	23.148564624950392	25.406799841248844	25.983595713718742	25.46103982008202
40-41	20.003439289918912	27.782848525735147	25.666362421789223	26.547349762556717
42-43	18.052303662843762	27.59501038400995	25.915049539002872	28.43763641414342
44-45	17.584961570515787	25.745770110989113	28.923313005172435	27.74595531332266
46-47	18.938832742473146	28.10863008624795	27.228953912905446	25.723583258373463
48-49	19.785176660449487	28.38869267298967	25.21396351707078	26.612167149490055
50-51	22.94891128925576	28.147736592851285	22.63142577453237	26.271926343360587
52-53	16.643956131183106	29.996428051700647	25.2139861620077	28.145629655108547
54-55	18.1085800460354	27.037860147630767	25.585363917771254	29.268195888562587
56-57	19.864001375861566	28.284538755639048	24.403021603672492	27.44843826482689
58-59	19.44359927506515	28.09651687325546	24.035294273279273	28.424589578400116
60-61	17.84542519976716	26.591522463883155	24.685135206646557	30.877917129703125
62-63	19.172917410802874	26.72804963553862	24.575676998584488	29.523355955074017
64-65	20.1918370046967	26.526427201164253	23.126281669643447	30.155454124495602
66-67	18.389998677073688	30.670723640693215	21.35732239714248	29.581955285090622
68-69	18.773231742000345	32.73674881278358	20.473034644231912	28.01698480098417
70-71	19.590465356226357	30.54578163443477	20.92251117754438	28.94124183179449
72-73	20.88971784594627	29.956215193724617	21.008770189293227	28.145296771035888
74-75	18.756779638595656	29.391221525517896	21.96867476254729	29.88332407333915
76	18.14381713318165	28.56235779670882	23.49330652415472	29.800518545954812
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	3.0
16	5.5
17	5.0
18	5.5
19	8.0
20	17.0
21	24.0
22	29.0
23	38.0
24	67.5
25	93.0
26	108.0
27	130.0
28	182.0
29	227.0
30	242.0
31	281.5
32	401.5
33	497.0
34	566.0
35	771.5
36	951.0
37	994.0
38	1098.5
39	1334.0
40	1593.0
41	1795.0
42	1869.0
43	2006.5
44	2216.5
45	2395.5
46	2502.0
47	2395.0
48	2292.0
49	2262.0
50	2228.0
51	2121.0
52	1897.5
53	1710.0
54	1639.0
55	1587.5
56	1374.5
57	1133.0
58	1053.0
59	904.0
60	698.5
61	592.0
62	542.0
63	473.5
64	370.5
65	309.5
66	271.5
67	260.0
68	213.5
69	150.0
70	131.5
71	130.0
72	117.0
73	88.0
74	79.5
75	87.0
76	74.0
77	59.5
78	50.0
79	42.0
80	39.5
81	33.5
82	29.0
83	28.0
84	24.5
85	20.0
86	13.0
87	7.0
88	8.5
89	8.5
90	6.0
91	5.0
92	5.0
93	2.5
94	0.0
95	1.5
96	3.0
97	2.0
98	2.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.005291145268393344
7	0.002645572634196672
8	0.002645572634196672
9	0.007936717902590016
10-11	0.005291145268393344
12-13	0.0
14-15	0.001322786317098336
16-17	0.001322786317098336
18-19	0.001322786317098336
20-21	0.0
22-23	0.003968358951295008
24-25	0.001322786317098336
26-27	0.002645572634196672
28-29	0.007936717902590016
30-31	0.001322786317098336
32-33	0.0
34-35	0.001322786317098336
36-37	0.015873435805180032
38-39	0.010582290536786687
40-41	0.001322786317098336
42-43	0.001322786317098336
44-45	0.006613931585491679
46-47	0.002645572634196672
48-49	0.001322786317098336
50-51	0.005291145268393344
52-53	0.011905076853885022
54-55	0.005291145268393344
56-57	0.011905076853885022
58-59	0.006613931585491679
60-61	0.013227863170983359
62-63	0.00925950421968835
64-65	0.017196222122278365
66-67	0.010582290536786687
68-69	0.001322786317098336
70-71	0.0
72-73	0.001322786317098336
74-75	0.005291145268393344
76	0.002645572634196672
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	37799.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.32479695230032
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.17859586697686	65.86417630096034
2	7.838374161762693	12.12201380988915
3	3.619816614205557	8.397047540940235
4	1.587518817572191	4.910182809069023
5	0.7971807855481047	3.082092118839123
6	0.44135760229916515	2.047673218868224
7	0.2326536198166142	1.2592925738776157
8	0.13001231695634324	0.8042540807957882
9	0.04105652114410839	0.28572184449324056
>10	0.13343369371835226	1.2275457022672558
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.005291145268393344	0.0
5	0.0	0.0	0.0	0.005291145268393344	0.0
6	0.0	0.0	0.0	0.005291145268393344	0.0
7	0.0	0.0	0.0	0.005291145268393344	0.0
8	0.0	0.0	0.0	0.005291145268393344	0.0
9	0.0	0.0	0.0	0.007936717902590016	0.0
10	0.0	0.0	0.0	0.007936717902590016	0.0
11	0.0	0.0	0.0	0.010582290536786687	0.0
12	0.0	0.0	0.0	0.013227863170983359	0.0
13	0.0	0.0	0.0	0.013227863170983359	0.0
14	0.0	0.0	0.0	0.013227863170983359	0.0
15	0.0	0.0	0.0	0.013227863170983359	0.0
16	0.0	0.0	0.0	0.013227863170983359	0.0
17	0.0	0.0	0.0	0.015873435805180032	0.0
18	0.0	0.0	0.0	0.023810153707770048	0.0
19	0.0	0.0	0.0	0.023810153707770048	0.0
20	0.0	0.0	0.0	0.023810153707770048	0.0
21	0.0	0.0	0.0	0.026455726341966718	0.0
22	0.0	0.0	0.0	0.052911452683933435	0.0
23	0.0	0.0	0.0	0.052911452683933435	0.0
24	0.0	0.0	0.0	0.07143046112331014	0.0
25	0.0	0.0	0.0	0.07143046112331014	0.0
26	0.0	0.0	0.0	0.07143046112331014	0.0
27	0.0	0.0	0.0	0.07143046112331014	0.0
28	0.0	0.0	0.0	0.08994946956268685	0.0
29	0.0	0.0	0.0	0.1137596232704569	0.0
30	0.0	0.0	0.0	0.1322786317098336	0.0
31	0.0	0.0	0.0	0.1481520675150136	0.0
32	0.0	0.0	0.0	0.17460779385698033	0.0
33	0.0	0.0	0.0	0.19577237493055372	0.0
34	0.0	0.0	0.0	0.22487367390671711	0.0
35	0.0	0.0	0.0	0.2804306992248472	0.0
36	0.0	0.0	0.0	0.3756713140559274	0.0
37	0.0	0.0	0.0	0.6031905605968412	0.0
38	0.0	0.0	0.0	0.8201275166009683	0.0
39	0.0	0.0	0.0	1.1905076853885024	0.0
40	0.0	0.0	0.0	1.4709383846133495	0.0
41	0.0	0.0	0.0	1.870419852377047	0.0
42	0.0	0.0	0.0	2.169369560041271	0.0
43	0.0	0.0	0.0	2.4286356781925447	0.0
44	0.0	0.0	0.0	2.7857879838090955	0.0
45	0.0	0.0	0.0	3.0741554009365326	0.0
46	0.0	0.0	0.0	3.44188999708987	0.0
47	0.0	0.0	0.0	3.9048652080742876	0.0
48	0.0	0.0	0.0	4.433979734913622	0.0
49	0.0	0.0	0.0	4.777904177359189	0.0
50	0.0	0.0	0.0	5.05568930394984	0.0
51	0.0	0.0	0.0	5.508082224397471	0.0
52	0.0	0.0	0.0	5.9446017090399215	0.0
53	0.0	0.0	0.0	6.185348818751819	0.0
54	0.0	0.0	0.0	6.47900738114765	0.0
55	0.0	0.0	0.0	6.8361596867642005	0.0
56	0.0	0.0	0.0	7.182729701843964	0.0
57	0.0	0.0	0.0	7.531945289557925	0.0
58	0.0	0.0	0.0	7.875869732003492	0.0
59	0.0	0.0	0.0	8.214503029180666	0.0
60	0.0	0.0	0.0	8.690706103336067	0.0
61	0.0	0.0	0.0	9.005529246805471	0.0
62	0.0	0.0	0.0	9.407656287203364	0.0
63	0.0	0.0	0.0	9.783327601259293	0.0
64	0.0	0.0	0.0	10.164290060583614	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGTGG	20	7.829863E-5	70.00001	45
CTTCCGA	15	0.0022082278	70.0	60
TACCTGA	25	2.3614342E-4	56.000004	9
CTTGTCA	20	0.0069003263	52.500004	1
CGGGTGG	20	0.0069003263	52.500004	10
TACCAGG	20	0.0069003263	52.500004	64
GAGTATC	20	0.0069003263	52.500004	28
GCGCCAA	20	0.0069003263	52.500004	47
AAGCCCG	20	0.0069003263	52.500004	15
ACATCCA	20	0.0069003263	52.500004	44
AGGTGTA	20	0.0069003263	52.500004	34
CTAGCAC	20	0.0069003263	52.500004	2
GGATATA	30	5.8070465E-4	46.666664	1
TTCCACC	30	5.8070465E-4	46.666664	46
CGCCGCC	30	5.8070465E-4	46.666664	16
GTGTAGA	45	9.033895E-5	38.88889	36
CCGTATC	45	0.004256389	31.111113	55
AGAGGTG	45	0.004256389	31.111113	32
ACGCCGC	50	0.007123751	28.000002	15
CGACGAA	50	0.007123751	28.000002	22
>>END_MODULE