FastQCFastQC Report
Wed 25 May 2016
SRR1779781_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779781_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37799
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT784820.76245403317548No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG10552.7910791290774886No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC4771.2619381465118125No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT4131.0926214979232254RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC3110.822773089235165No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC1550.41006375830048414No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC1480.39154474986110743RNA PCR Primer, Index 24 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT1350.3571523056165507No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT1330.3518611603481574No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1060.2804306992248472No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGT830.21958252863832375No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC770.20370909283314373No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG750.1984179475647504No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAGCTCGTATGCCGT620.16402550332019367No Hit
TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT600.1587343580518003RNA PCR Primer, Index 24 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCG510.13492420434403027No Hit
TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC460.12169634117304691No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG440.11640519590465356No Hit
CTTATACACATCTCCGAGCCCACGAGAAGTGATAGCATCTCGTATGCCGT420.11111405063626023No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAC150.00218917870.15272556
CTTGACA150.00218917870.15272557
TGATCTC150.00221237569.9668935
CTCTCCG150.00221237569.9668910
CCTTTTT150.00221237569.9668962
TTTACAC353.59978E-969.966892
TGCGGTA150.00221237569.9668944
CTTTTTG150.00221237569.9668963
CTTTATA650.059.2027552
TGCAAAA308.18316E-658.3830759
GCCTTTT200.00687703952.54476561
TTGTAAT200.0069132552.47516663
CAACTCG200.0069132552.47516636
GACGAAC200.0069132552.47516627
CTTAATA200.0069132552.4751662
TTTATAC800.052.4751663
ACGAACC200.0069132552.47516628
GGACGAA200.0069132552.47516626
GCATCTC11950.051.5237335
TCTCGTA12050.051.3864738
Produced by FastQC (version 0.11.5)