##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779781_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37799
Sequences flagged as poor quality	0
Sequence length	76
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.38310537315802	34.0	34.0	34.0	34.0	34.0
2	33.55390354242176	34.0	34.0	34.0	34.0	34.0
3	33.69493901955078	34.0	34.0	34.0	34.0	34.0
4	33.439032778644936	34.0	34.0	34.0	34.0	34.0
5	33.49054207783275	34.0	34.0	34.0	34.0	34.0
6	37.10545252519908	38.0	37.0	38.0	36.0	38.0
7	37.2066456784571	38.0	38.0	38.0	36.0	38.0
8	37.04613878674039	38.0	37.0	38.0	36.0	38.0
9	36.942882086827694	38.0	37.0	38.0	35.0	38.0
10-11	36.89035424217572	38.0	37.0	38.0	35.0	38.0
12-13	37.058612661710626	38.0	37.5	38.0	35.5	38.0
14-15	37.13014894573931	38.0	37.5	38.0	36.0	38.0
16-17	36.9612952723617	38.0	37.0	38.0	35.0	38.0
18-19	36.76049630942617	38.0	37.0	38.0	34.0	38.0
20-21	36.841622794253816	38.0	37.0	38.0	34.5	38.0
22-23	36.82994259107384	38.0	37.0	38.0	34.5	38.0
24-25	37.009166909177495	38.0	37.0	38.0	35.0	38.0
26-27	36.84182121220138	38.0	37.0	38.0	34.5	38.0
28-29	36.16381385750946	38.0	37.0	38.0	34.0	38.0
30-31	36.58092806688008	38.0	37.0	38.0	34.0	38.0
32-33	36.7546495939046	38.0	37.0	38.0	34.5	38.0
34-35	36.778221646075295	38.0	37.0	38.0	34.5	38.0
36-37	36.621537606815	38.0	37.0	38.0	34.0	38.0
38-39	35.15779517976666	38.0	36.0	38.0	29.0	38.0
40-41	36.06863938199423	38.0	36.5	38.0	32.5	38.0
42-43	36.847099129606605	38.0	37.0	38.0	34.5	38.0
44-45	36.50433873912009	38.0	37.0	38.0	34.0	38.0
46-47	36.715931638403134	38.0	37.0	38.0	34.5	38.0
48-49	36.19119553427339	38.0	37.0	38.0	33.5	38.0
50-51	36.29246805471044	38.0	37.0	38.0	33.5	38.0
52-53	36.22534987698087	38.0	37.0	38.0	34.0	38.0
54-55	36.220653985555174	38.0	37.0	38.0	34.0	38.0
56-57	36.12971242625466	38.0	37.0	38.0	33.0	38.0
58-59	36.213167015000394	38.0	37.0	38.0	34.0	38.0
60-61	35.65255694595095	38.0	36.5	38.0	31.0	38.0
62-63	35.541694224714945	38.0	36.0	38.0	31.0	38.0
64-65	36.093454853303	38.0	37.0	38.0	34.0	38.0
66-67	35.79446546204926	38.0	36.5	38.0	32.5	38.0
68-69	35.67204158840181	38.0	36.5	38.0	31.0	38.0
70-71	33.832125188497045	37.5	33.5	38.0	19.5	38.0
72-73	30.684819704224978	37.0	23.5	38.0	11.0	38.0
74-75	30.13665705441943	37.0	23.0	38.0	11.0	38.0
76	29.91348977486177	37.0	23.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	3.0
24	11.0
25	22.0
26	57.0
27	95.0
28	194.0
29	355.0
30	562.0
31	961.0
32	1489.0
33	2296.0
34	3857.0
35	6947.0
36	6816.0
37	14133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.068445291408604	15.71118522047536	13.006193425440685	44.21417606267535
2	13.368078520595784	15.439561893171778	50.51191830471705	20.68044128151538
3	12.846900711659037	18.90526204396942	49.765866821873594	18.48197042249795
4	8.968491229926718	37.35548559485701	33.072303500092595	20.60371967512368
5	8.357363951427287	30.524617053361204	50.951083361993696	10.166935633217811
6	18.016349638879337	53.16807322945052	17.27294372867007	11.54263340300008
7	17.558665573163314	26.35519458186725	19.513743749834653	36.57239609513479
8	15.616815259662953	51.71565385327654	15.14325775814175	17.524273128918754
9	16.4554617847033	17.55337442789492	15.003042408529327	50.98812137887245
10-11	16.578480912193445	33.43871530992884	31.521997936453346	18.460805841424378
12-13	16.857588825101192	17.644646683774702	33.44797481414852	32.04978967697558
14-15	16.507050451070135	17.44093759094156	19.493901955078176	46.558110002910134
16-17	30.260059789941536	30.67541469351041	16.747797560782033	22.316727955766027
18-19	30.912193444271015	22.13815180295775	17.840419058705255	29.109235694065983
20-21	16.55599354480277	19.147331939998413	17.694912563824438	46.60176195137438
22-23	16.681658244927114	32.634461229133045	17.840419058705255	32.84346146723458
24-25	29.983597449667982	32.93076536416307	17.959469827244106	19.12616735892484
26-27	30.74023122304823	34.409640466679015	18.78356570279637	16.06656260747639
28-29	29.679356596735364	22.139474589274847	19.042831820947644	29.138336993042145
30-31	31.01537077700468	19.282256144342444	33.36860763512262	16.33376544353025
32-33	19.135426863144527	33.01013254318897	32.05904918119527	15.79539141247123
34-35	27.799677240138625	34.23503267282203	22.545569988624038	15.4197200984153
36-37	18.819280933358026	31.84475779782534	19.107648350485462	30.228312918331174
38-39	15.536125294319955	21.769094420487313	32.61991057964496	30.074869705547762
40-41	15.579777242784202	18.319267705494855	35.09748935157015	31.003465700150795
42-43	29.583184517904147	18.135640303186634	33.47487334153472	18.806301837374498
44-45	16.389322468848384	32.17016323183153	34.8223497976137	16.618164501706396
46-47	29.50474880287838	19.065319188338318	23.195058070319323	28.234873938463977
48-49	28.181962485780048	17.993862271488663	20.106352019894704	33.717823222836586
50-51	17.881758410392774	17.8433940548478	33.98288155997407	30.291965974785356
52-53	14.721409952763405	17.207616073673208	47.98814454132871	20.082829432234675
54-55	14.201394862565012	18.04057541389304	37.27485674205629	30.483172981485644
56-57	28.55214399152991	17.717046056114345	23.799629433562732	29.93118051879301
58-59	16.027574891499945	18.679210331322114	45.42447337779189	19.868741399386046
60-61	28.150510035325404	32.16331714803594	22.809361893547493	16.87681092309117
62-63	14.986768986504368	49.52897591955544	18.302460968510186	17.18179412543001
64-65	13.93853603037479	51.35932476947704	18.05553718133591	16.646602018812263
66-67	13.639490884072927	51.299251144452384	17.98576380619724	17.075494165277448
68-69	13.955949467557376	51.36583107348369	17.46544083603413	17.212778622924795
70-71	14.710940600608547	48.24844556158222	18.358248445561582	18.68236539224765
72-73	15.755146319521618	42.13631793406361	18.594221304969043	23.514314441445734
74-75	15.87295287985819	39.27163531497209	19.89575892266582	24.959652882503903
76	15.80242340864596	40.298428488279804	19.54600772527647	24.353140377797764
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	2.0
17	4.0
18	8.5
19	10.5
20	9.0
21	10.0
22	24.5
23	37.0
24	63.5
25	92.0
26	91.5
27	121.0
28	174.5
29	198.0
30	244.5
31	297.0
32	376.5
33	450.0
34	498.5
35	641.0
36	849.5
37	964.0
38	1046.0
39	1266.5
40	2596.0
41	4614.5
42	5442.0
43	5131.0
44	4274.0
45	3117.0
46	2506.0
47	2199.5
48	1742.0
49	1455.0
50	1319.0
51	1267.5
52	1062.5
53	841.5
54	774.0
55	743.5
56	623.0
57	464.0
58	395.0
59	330.5
60	235.5
61	195.5
62	186.0
63	163.0
64	126.5
65	99.0
66	85.5
67	86.0
68	91.5
69	84.5
70	70.0
71	68.0
72	66.0
73	59.5
74	48.0
75	41.0
76	40.5
77	41.5
78	41.0
79	39.0
80	36.5
81	29.0
82	23.0
83	22.0
84	16.5
85	9.0
86	6.0
87	5.0
88	4.0
89	2.5
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04497473478134342
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.001322786317098336
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.00925950421968835
52-53	0.027778512659065058
54-55	0.046297521098441756
56-57	0.05026588004973676
58-59	0.02910129897616339
60-61	0.019841794756475038
62-63	0.023810153707770045
64-65	0.011905076853885022
66-67	0.021164581073573375
68-69	0.003968358951295008
70-71	0.010582290536786687
72-73	0.013227863170983359
74-75	0.005291145268393344
76	0.002645572634196672
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	37799.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.04584777375063
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.15098871187255	17.894653297706288
2	19.822272035865822	13.100875684541919
3	10.855816187655112	10.762189475912061
4	6.172444159795052	8.158946003862535
5	3.498518933632215	5.780576205719727
6	2.0814986790489156	4.127093309346808
7	0.9927147546233288	2.2963570464827114
8	0.7525418301176847	1.9894706209158972
9	0.5043631414618525	1.5000396835895131
>10	1.0407493395244578	4.788486467895976
>50	0.04803458490112881	1.0793936347522421
>100	0.06404611320150508	4.96838540702135
>500	0.0	0.0
>1k	0.008005764150188135	2.7910791290774886
>5k	0.008005764150188135	20.76245403317548
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT	7848	20.76245403317548	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	1055	2.7910791290774886	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	477	1.2619381465118125	No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT	413	1.0926214979232254	RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	311	0.822773089235165	No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	155	0.41006375830048414	No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	148	0.39154474986110743	RNA PCR Primer, Index 24 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT	135	0.3571523056165507	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT	133	0.3518611603481574	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	106	0.2804306992248472	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGT	83	0.21958252863832375	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	77	0.20370909283314373	No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	75	0.1984179475647504	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAGCTCGTATGCCGT	62	0.16402550332019367	No Hit
TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT	60	0.1587343580518003	RNA PCR Primer, Index 24 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCG	51	0.13492420434403027	No Hit
TTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	46	0.12169634117304691	No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	44	0.11640519590465356	No Hit
CTTATACACATCTCCGAGCCCACGAGAAGTGATAGCATCTCGTATGCCGT	42	0.11111405063626023	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.005291145268393344	0.0
5	0.0	0.0	0.0	0.007936717902590016	0.0
6	0.0	0.0	0.0	0.007936717902590016	0.0
7	0.0	0.0	0.0	0.007936717902590016	0.0
8	0.0	0.0	0.0	0.007936717902590016	0.0
9	0.0	0.0	0.0	0.007936717902590016	0.0
10	0.0	0.0	0.0	0.007936717902590016	0.0
11	0.0	0.0	0.0	0.010582290536786687	0.0
12	0.0	0.0	0.0	0.013227863170983359	0.0
13	0.0	0.0	0.0	0.013227863170983359	0.0
14	0.0	0.0	0.0	0.013227863170983359	0.0
15	0.0	0.0	0.0	0.013227863170983359	0.0
16	0.0	0.0	0.0	0.013227863170983359	0.0
17	0.0	0.0	0.0	0.013227863170983359	0.0
18	0.0	0.0	0.0	0.021164581073573375	0.0
19	0.0	0.0	0.0	0.021164581073573375	0.0
20	0.0	0.0	0.0	0.021164581073573375	0.0
21	0.0	0.0	0.0	0.023810153707770048	0.0
22	0.0	0.0	0.0	0.031746871610360064	0.0
23	0.0	0.0	0.0	0.031746871610360064	0.0
24	0.0	0.0	0.0	0.04497473478134342	0.0
25	0.0	0.0	0.0	0.04497473478134342	0.0
26	0.0	0.0	0.0	0.04497473478134342	0.0
27	0.0	0.0	0.0	0.04497473478134342	0.0
28	0.0	0.0	0.0	0.060848170586523455	0.0
29	0.0	0.0	0.0	0.0740760337575068	0.0
30	0.0	0.0	0.0	0.08994946956268685	0.0
31	0.0	0.0	0.0	0.10582290536786687	0.0
32	0.0	0.0	0.0	0.1296330590756369	0.0
33	0.0	0.0	0.0	0.1507976401492103	0.0
34	0.0	0.0	0.0	0.18254451175957037	0.0
35	0.0	0.0	0.0	0.24074710971189714	0.0
36	0.0	0.0	0.0	0.34392444244556736	0.0
37	0.0	0.0	0.0	0.5767348342548745	0.0
38	0.0	0.0	0.0	0.7963173628931982	0.0
39	0.0	0.0	0.0	1.1481785232413555	0.0
40	0.0	0.0	0.0	1.4233180771978096	0.0
41	0.0	0.0	0.0	1.8280906902299003	0.0
42	0.0	0.0	0.0	2.1270403978941244	0.0
43	0.0	0.0	0.0	2.3730786528744146	0.0
44	0.0	0.0	0.0	2.724939813222572	0.0
45	0.0	0.0	0.0	3.0159528029842058	0.0
46	0.0	0.0	0.0	3.383687399137543	0.0
47	0.0	0.0	0.0	3.830789174316781	0.0
48	0.0	0.0	0.0	4.357258128521918	0.0
49	0.0	0.0	0.0	4.709119288870076	0.0
50	0.0	0.0	0.0	4.97632212492394	0.0
51	0.0	0.0	0.0	5.415487182200588	0.0
52	0.0	0.0	0.0	5.836133231037858	0.0
53	0.0	0.0	0.0	6.076880340749756	0.0
54	0.0	0.0	0.0	6.362602185242996	0.0
55	0.0	0.0	0.0	6.70917220032276	0.0
56	0.0	0.0	0.0	7.0530966427683275	0.0
57	0.0	0.0	0.0	7.397021085213894	0.0
58	0.0	0.0	0.0	7.730363237122675	0.0
59	0.0	0.0	0.0	8.050477525860472	0.0
60	0.0	0.0	0.0	8.51345273684489	0.0
61	0.0	0.0	0.0	8.81504801714331	0.0
62	0.0	0.0	0.0	9.209238339638615	0.0
63	0.0	0.0	0.0	9.590200798962936	0.0
64	0.0	0.0	0.0	9.965872113018863	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTTGAC	15	0.002189178	70.152725	56
CTTGACA	15	0.002189178	70.152725	57
TGATCTC	15	0.002212375	69.96689	35
CTCTCCG	15	0.002212375	69.96689	10
CCTTTTT	15	0.002212375	69.96689	62
TTTACAC	35	3.59978E-9	69.96689	2
TGCGGTA	15	0.002212375	69.96689	44
CTTTTTG	15	0.002212375	69.96689	63
CTTTATA	65	0.0	59.202755	2
TGCAAAA	30	8.18316E-6	58.38307	59
GCCTTTT	20	0.006877039	52.544765	61
TTGTAAT	20	0.00691325	52.475166	63
CAACTCG	20	0.00691325	52.475166	36
GACGAAC	20	0.00691325	52.475166	27
CTTAATA	20	0.00691325	52.475166	2
TTTATAC	80	0.0	52.475166	3
ACGAACC	20	0.00691325	52.475166	28
GGACGAA	20	0.00691325	52.475166	26
GCATCTC	1195	0.0	51.52373	35
TCTCGTA	1205	0.0	51.38647	38
>>END_MODULE