FastQCFastQC Report
Wed 25 May 2016
SRR1779780_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779780_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136084
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACA150.002218689570.0158137
TTCGGTA207.909109E-569.9900826
CCGGAAT150.00222192869.9900743
CGTTACT150.00222192869.99007417
GGGCGCC150.00222192869.9900749
GCCTAAA308.3476225E-658.325061
CGTTCGG252.3851958E-455.9920624
GTTCGGT252.3851958E-455.9920625
GTAGATC750.055.9920638
TCGCCGT200.006932737352.5118652
TCGTTCG200.006942827352.4925623
GTACATG200.006942827352.492561
GACGTTA200.006942827352.4925615
GGCGCCG200.006942827352.4925610
GGTCTAT200.006942827352.4925627
GACGATC200.006942827352.4925623
TGTACTT200.006942827352.4925610
TACCGTC200.006942827352.492567
GACCGCG305.8545166E-446.67720859
CGACGAT451.6764116E-646.6600522