##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779780_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	136084
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.020244848769877	34.0	31.0	34.0	12.0	34.0
2	30.694350548190823	34.0	32.0	34.0	21.0	34.0
3	30.280811851503483	34.0	32.0	34.0	11.0	34.0
4	30.349614943711238	34.0	32.0	34.0	12.0	34.0
5	30.954550130801564	34.0	33.0	34.0	21.0	34.0
6	33.78026072131919	38.0	34.0	38.0	21.0	38.0
7	33.99332030216631	38.0	35.0	38.0	21.0	38.0
8	33.27286822844714	38.0	34.0	38.0	11.0	38.0
9	34.09293524587755	38.0	36.0	38.0	21.0	38.0
10-11	32.763385115075984	37.5	30.5	38.0	16.5	38.0
12-13	33.71689177272861	38.0	34.0	38.0	21.0	38.0
14-15	32.312637782546076	37.0	30.0	38.0	11.0	38.0
16-17	33.02828400105817	37.5	32.5	38.0	16.0	38.0
18-19	33.931788454190055	38.0	35.0	38.0	21.0	38.0
20-21	32.640549219599656	37.5	30.5	38.0	16.0	38.0
22-23	33.5553702125158	38.0	35.0	38.0	16.0	38.0
24-25	33.86221010552306	38.0	35.0	38.0	21.0	38.0
26-27	32.74707533582199	38.0	32.5	38.0	11.0	38.0
28-29	33.99323212133682	38.0	35.0	38.0	21.0	38.0
30-31	34.32549381264513	38.0	37.0	38.0	21.0	38.0
32-33	34.430781723053414	38.0	37.0	38.0	21.0	38.0
34-35	34.43647306075659	38.0	36.5	38.0	22.0	38.0
36-37	33.93974677405132	38.0	35.5	38.0	21.0	38.0
38-39	33.91701449104964	38.0	35.0	38.0	21.0	38.0
40-41	33.92092751535816	38.0	35.5	38.0	21.0	38.0
42-43	34.2338555598013	38.0	36.5	38.0	21.5	38.0
44-45	34.3632792980806	38.0	36.5	38.0	21.5	38.0
46-47	34.26666617677317	38.0	36.0	38.0	21.0	38.0
48-49	34.03230357720231	38.0	35.5	38.0	21.0	38.0
50-51	33.437141765380204	38.0	35.0	38.0	16.0	38.0
52-53	34.011897063578374	38.0	35.5	38.0	21.0	38.0
54-55	34.13062520208107	38.0	36.0	38.0	21.0	38.0
56-57	34.161554628023865	38.0	36.0	38.0	21.0	38.0
58-59	34.317847799888305	38.0	37.0	38.0	21.5	38.0
60-61	34.25541577261103	38.0	36.0	38.0	21.0	38.0
62-63	34.21094324093942	38.0	36.0	38.0	21.0	38.0
64-65	34.27158593221833	38.0	36.5	38.0	21.0	38.0
66-67	34.06386129155521	38.0	36.0	38.0	21.0	38.0
68-69	33.450475441638986	38.0	34.0	38.0	15.5	38.0
70-71	33.45731313012551	38.0	34.0	38.0	18.5	38.0
72-73	33.768268128508865	38.0	34.5	38.0	20.5	38.0
74-75	33.87869624643602	38.0	35.0	38.0	21.0	38.0
76	32.932894388759884	37.0	33.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	22.0
13	323.0
14	1103.0
15	2018.0
16	2619.0
17	2771.0
18	2562.0
19	2168.0
20	1587.0
21	1212.0
22	906.0
23	742.0
24	642.0
25	684.0
26	787.0
27	892.0
28	1108.0
29	1467.0
30	1789.0
31	2404.0
32	3331.0
33	4755.0
34	7256.0
35	11998.0
36	23951.0
37	56987.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.21560212809735	19.36671467622939	11.963934040739543	28.453749154933718
2	21.333882014050147	22.855001322712443	31.166044501925285	24.645072161312132
3	18.599541459686662	21.128861585491315	31.00805384909321	29.26354310572881
4	13.238881867082098	22.567678786631788	32.69598189353635	31.49745745274977
5	13.028717556803151	35.49351870903266	34.312630434143614	17.165133300020578
6	27.15421584044914	38.602460281300985	18.058229596860716	16.185094281389162
7	26.597785110119858	29.410122061449602	22.1231784478134	21.86891438061714
8	23.087210840363305	37.8281061697187	18.375415184738838	20.709267805179156
9	23.987477125912207	19.772765688500858	19.79334318113338	36.44641400445356
10-11	23.129804088711218	26.32126218015608	24.843109301744537	25.705824429388162
12-13	23.218012404103348	23.818009464742364	26.08940066429558	26.874577466858707
14-15	24.06022772782887	25.198132029232053	23.291214585162788	27.45042565777629
16-17	24.815278854526817	26.505417629617114	23.635121083746377	25.044182432109697
18-19	21.913993033612087	26.45353536838083	26.09860231331109	25.533869284695992
20-21	23.371226595338175	26.159577907762856	25.79546456600335	24.673730930895623
22-23	24.870207410945568	25.810078445060903	24.614847610824317	24.704866533169216
24-25	21.895128251292434	28.243943842064073	24.764018356781463	25.09690954986203
26-27	22.711340054672114	27.904088651127246	25.62167484788807	23.762896446312574
28-29	22.016086657774544	27.531039753666164	26.602511105966908	23.850362482592384
30-31	22.50897426947426	26.957713462690187	26.261082350174707	24.272229917660848
32-33	21.204550130801564	27.965080391522886	26.125775256459242	24.704594221216308
34-35	21.46576082363618	28.650164058509915	26.219232140035786	23.66484297781811
36-37	23.515533702290917	26.74464754261019	25.62344277115075	24.116375983948142
38-39	23.06519294790296	26.00515899553916	26.893652671727676	24.0359953848302
40-41	21.85042841815964	26.65414970385503	26.69824076659661	24.79718111138872
42-43	20.431506119494568	26.414685317254733	27.200243971443584	25.953564591807115
44-45	20.459589353165097	24.82105851056012	29.024162612619232	25.69518952365555
46-47	20.723332488232895	25.560246475380023	28.244358954574018	25.472062081813075
48-49	20.698840776734702	26.239229878933735	28.225892381459776	24.836036962871788
50-51	22.598637556125485	25.97057591546087	26.726019444587333	24.704767083826308
52-53	18.72329010755612	27.18740928282446	27.81356449141425	26.275736118205167
54-55	19.11547478191853	26.36966922166778	27.836161472150977	26.678694524262713
56-57	19.586443094584514	26.364895802327588	27.28321702734385	26.765444075744043
58-59	19.554848382402657	26.465097820175504	27.171372716181853	26.80868108123999
60-61	18.735553195476978	25.89749337605974	27.864647452033854	27.50230597642943
62-63	19.565880420523488	26.399938266387885	26.995226668920434	27.038954644168196
64-65	20.251587581584054	25.956958899276767	25.767698594696302	28.02375492444288
66-67	19.656847613587924	27.31989828166571	24.938998397789245	28.084255706957123
68-69	19.864053056050558	27.71774475042713	24.600518068083698	27.817684125438614
70-71	21.067812054496553	26.658926235652032	24.47090724710101	27.8023544627504
72-73	21.60300126399953	26.87872953349599	23.898409712219642	27.619859490284842
74-75	21.44991015686721	26.805589749431363	23.763049301648778	27.981450792052648
76	21.15740740740741	26.078777189888303	24.496619635508523	28.26719576719577
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.5
12	1.0
13	0.5
14	1.0
15	2.0
16	6.5
17	11.0
18	16.0
19	27.5
20	35.5
21	37.0
22	58.0
23	115.5
24	192.0
25	232.0
26	305.0
27	486.5
28	626.0
29	657.0
30	810.5
31	1149.5
32	1606.5
33	1878.0
34	2193.5
35	2825.5
36	3489.0
37	3836.0
38	4348.5
39	5608.0
40	6789.0
41	7350.5
42	7478.0
43	7733.5
44	8655.5
45	9294.0
46	9266.0
47	9099.0
48	8782.5
49	8169.5
50	7706.0
51	7380.5
52	6526.0
53	5449.5
54	4902.0
55	4544.5
56	3816.5
57	3109.5
58	2773.0
59	2446.5
60	1918.0
61	1598.0
62	1480.0
63	1379.0
64	1148.5
65	981.5
66	847.0
67	750.0
68	699.0
69	640.0
70	576.0
71	520.0
72	512.5
73	480.5
74	412.5
75	369.0
76	331.0
77	272.0
78	244.0
79	237.0
80	215.5
81	190.0
82	152.0
83	118.0
84	106.5
85	84.5
86	73.5
87	73.0
88	53.0
89	30.0
90	19.5
91	15.0
92	18.0
93	15.5
94	10.0
95	5.0
96	3.0
97	4.5
98	7.5
99	8.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0014696804914611564
7	0.0036742012286528907
8	0.0
9	0.009552923194497517
10-11	0.0014696804914611564
12-13	0.0
14-15	3.674201228652891E-4
16-17	3.674201228652891E-4
18-19	0.0014696804914611564
20-21	0.0
22-23	0.0011022603685958672
24-25	0.002571940860057024
26-27	0.0
28-29	0.006246142088709915
30-31	3.674201228652891E-4
32-33	0.0
34-35	0.0033067811057876017
36-37	0.018371006143264455
38-39	0.00808324270303636
40-41	0.0014696804914611564
42-43	0.0018371006143264453
44-45	0.004409041474383469
46-47	0.00404162135151818
48-49	0.0011022603685958672
50-51	0.0036742012286528907
52-53	0.011390023808823962
54-55	0.00808324270303636
56-57	0.015064225037476854
58-59	0.013227124423150407
60-61	0.018003586020399167
62-63	0.011390023808823962
64-65	0.02057552688045619
66-67	0.01616648540607272
68-69	0.0011022603685958672
70-71	0.0014696804914611564
72-73	0.005878721965844626
74-75	0.009920343317362804
76	0.002939360982922313
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	136084.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.36577408071485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.5618077093487	46.982745951030246
2	10.42163120142739	12.790629317186445
3	5.307212396268666	9.770435907233766
4	2.968542312804608	7.286675876664413
5	1.5148067872923876	4.647864554245907
6	0.9184638781448706	3.381734810852121
7	0.577183297608641	2.479350989094971
8	0.43588116250943015	2.1398547955674436
9	0.2646421343807254	1.46159724875812
>10	1.0298291202145875	9.059110549366569
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.348402457305782E-4	0.0
2	0.0	0.0	0.0	7.348402457305782E-4	0.0
3	0.0	0.0	0.0	7.348402457305782E-4	0.0
4	0.0	0.0	0.0	0.012492284177419828	0.0
5	0.0	0.0	0.0	0.013227124423150407	0.0
6	0.0	0.0	0.0	0.013961964668880986	0.0
7	0.0	0.0	0.0	0.013961964668880986	0.0
8	0.0	0.0	0.0	0.013961964668880986	0.0
9	0.0	0.0	0.0	0.015431645160342141	0.0
10	0.0	0.0	0.0	0.016901325651803297	0.0
11	0.0	0.0	0.0	0.017636165897533876	0.0
12	0.0	0.0	0.0	0.017636165897533876	0.0
13	0.0	0.0	0.0	0.019105846388995033	0.0
14	0.0	0.0	0.0	0.019105846388995033	0.0
15	0.0	0.0	0.0	0.020575526880456187	0.0
16	0.0	0.0	0.0	0.021310367126186766	0.0
17	0.0	0.0	0.0	0.021310367126186766	0.0
18	0.0	0.0	0.0	0.022780047617647924	0.0
19	0.0	0.0	0.0	0.02424972810910908	0.0
20	0.0	0.0	0.0	0.02865876958349255	0.0
21	0.0	0.0	0.0	0.030863290320684283	0.0
22	0.0	0.0	0.0	0.03821169277799007	0.0
23	0.0	0.0	0.0	0.0404162135151818	0.0
24	0.0	0.0	0.0	0.041151053760912375	0.0
25	0.0	0.0	0.0	0.041151053760912375	0.0
26	0.0	0.0	0.0	0.04482525498956527	0.0
27	0.0	0.0	0.0	0.07715822580171071	0.0
28	0.0	0.0	0.0	0.10361247464801152	0.0
29	0.0	0.0	0.0	0.13080156374004293	0.0
30	0.0	0.0	0.0	0.15211193086622968	0.0
31	0.0	0.0	0.0	0.19252814438141147	0.0
32	0.0	0.0	0.0	0.2189823932277123	0.0
33	0.0	0.0	0.0	0.2498456835483966	0.0
34	0.0	0.0	0.0	0.30422386173245936	0.0
35	0.0	0.0	0.0	0.3630110813909056	0.0
36	0.0	0.0	0.0	0.522471414714441	0.0
37	0.0	0.0	0.0	0.8619676082419682	0.0
38	0.0	0.0	0.0	1.325651803297963	0.0
39	0.0	0.0	0.0	1.93189500602569	0.0
40	0.0	0.0	0.0	2.602804150377708	0.0
41	0.0	0.0	0.0	3.4067193792069603	0.0
42	0.0	0.0	0.0	4.456071250110226	0.0
43	0.0	0.0	0.0	5.624467240821845	0.0
44	0.0	0.0	0.0	6.544487228476529	0.0
45	0.0	0.0	0.0	7.432909085564798	0.0
46	0.0	0.0	0.0	8.223597189970901	0.0
47	0.0	0.0	0.0	9.015754974868464	0.0
48	0.0	0.0	0.0	9.775579788953882	0.0
49	0.0	0.0	0.0	10.42150436495106	0.0
50	0.0	0.0	0.0	11.074042503159813	0.0
51	0.0	0.0	0.0	11.926457188207284	0.0
52	0.0	0.0	0.0	13.085300255724405	0.0
53	0.0	0.0	0.0	14.31101378560301	0.0
54	0.0	0.0	0.0	15.360365656506275	0.0
55	0.0	0.0	0.0	16.10255430469416	0.0
56	0.0	0.0	0.0	16.838129390670467	0.0
57	0.0	0.0	0.0	17.572969636401048	0.0
58	0.0	0.0	0.0	18.257105905176214	0.0
59	0.0	0.0	0.0	18.90009112019047	0.0
60	0.0	0.0	0.0	19.513682725375503	0.0
61	0.0	0.0	0.0	20.135357573263573	0.0
62	0.0	0.0	0.0	20.804062196878398	0.0
63	0.0	0.0	0.0	21.515387554745597	0.0
64	0.0	0.0	0.0	22.287704653008436	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTACA	15	0.0022186895	70.01581	37
TTCGGTA	20	7.909109E-5	69.99008	26
CCGGAAT	15	0.002221928	69.990074	3
CGTTACT	15	0.002221928	69.990074	17
GGGCGCC	15	0.002221928	69.990074	9
GCCTAAA	30	8.3476225E-6	58.32506	1
CGTTCGG	25	2.3851958E-4	55.99206	24
GTTCGGT	25	2.3851958E-4	55.99206	25
GTAGATC	75	0.0	55.99206	38
TCGCCGT	20	0.0069327373	52.51186	52
TCGTTCG	20	0.0069428273	52.49256	23
GTACATG	20	0.0069428273	52.49256	1
GACGTTA	20	0.0069428273	52.49256	15
GGCGCCG	20	0.0069428273	52.49256	10
GGTCTAT	20	0.0069428273	52.49256	27
GACGATC	20	0.0069428273	52.49256	23
TGTACTT	20	0.0069428273	52.49256	10
TACCGTC	20	0.0069428273	52.49256	7
GACCGCG	30	5.8545166E-4	46.677208	59
CGACGAT	45	1.6764116E-6	46.66005	22
>>END_MODULE