FastQCFastQC Report
Wed 25 May 2016
SRR1779780_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779780_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136084
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT103357.5945739396255245No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG15641.1492901443226242No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC6400.47029775726757No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT5310.39020017048293704TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC3620.26601216895446933No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT1750.12859704300285116No Hit
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC1680.12345316128273714RNA PCR Primer, Index 12 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC1580.11610475882543134TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT1500.11022603685958672No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT1420.1043473148937421No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCC150.002218736370.0154456
CAACCGT150.002221974869.989719
TTGAAAG252.385258E-455.99176832
ACGTTTT200.00693288252.5115870
CGTGAGC200.00693288252.5115846
ATACAGC200.00693288252.5115861
TTTACGT200.00694297252.49228332
CTACGGA200.00694297252.4922833
AAGGGCC200.00694297252.49228310
CCAAAAG200.00694297252.49228324
CCGCCGA200.00694297252.49228338
AATCTCG16700.047.35830336
ATCTCGT16950.047.27918237
TCTCGTA17050.047.0018938
GCCGTCT18100.046.80590446
CTGCTTG17750.046.74270254
CGTATGC18200.046.723941
CTCGTAT18100.046.59535639
CGTCTTC17900.046.54657748
TGCCGTC18250.046.42119645
Produced by FastQC (version 0.11.5)