FastQCFastQC Report
Wed 25 May 2016
SRR1779779_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779779_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323431
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT401.0631993E-861.2559652
ACGATAC352.0829195E-550.00485624
CGACGAT651.8553692E-1048.45126722
CTCGGTG800.048.1222444
GCGAAGT305.8702886E-446.671232
ATCCCGG451.6826725E-646.66398642
TCGGTGG900.046.66398645
CGACGCT551.3108911E-744.5360113
CGGTGGT953.6379788E-1240.5302646
GTGGACG350.001253831540.00388748
CGGTCGC350.001253831540.00388749
CCTCGGT350.001254785639.99770443
GGTGCAA350.001255740239.9915161
GGTGGAC459.181972E-538.8926747
TATCACG459.190342E-538.88665453
ATCTCGG901.05501385E-1038.88665442
CATGACG459.198719E-538.88064611
CGACGAA1300.037.6843222
GATCTCG852.568413E-937.05669441
GTAGATC1350.036.29421238