FastQCFastQC Report
Wed 25 May 2016
SRR1779779_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779779_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323431
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT200356.1945206241825925TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG26000.8038808895869599TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC15470.47830912930424113RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT5170.15984862304479164TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC4560.14098834063525142RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT3310.10234022094357065No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGC200.006953735452.48817419
ACGTGTA200.006953735452.48817418
GCATGTC200.006953735452.48817433
TTTTCCG200.006953735452.48817417
ATCTCGT28500.051.9356737
TCTCGTA28400.051.87211238
GATCTCG28550.051.7221436
CCGTCTT29950.051.5481947
GCCGTCT30150.051.43847746
CGTCTTC30100.051.4155748
TATGCCG30150.051.41461643
CGTATGC30400.051.33711641
TCGTATG30400.051.10690340
CTCGTAT29950.050.9401139
CTGCTTG30250.050.82909854
ATGCCGT30500.050.8246144
TGCCGTC30700.050.7371645
GTATGCC30600.050.6585242
TGATCTC29050.050.47055435
GTCTTCT30850.050.06759349
Produced by FastQC (version 0.11.5)