##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779779_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	323431
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.57915289505335	34.0	34.0	34.0	34.0	34.0
2	33.66405199254246	34.0	34.0	34.0	34.0	34.0
3	33.76843283420575	34.0	34.0	34.0	34.0	34.0
4	33.57327219716107	34.0	34.0	34.0	34.0	34.0
5	33.62844316098333	34.0	34.0	34.0	34.0	34.0
6	37.31112663906676	38.0	38.0	38.0	37.0	38.0
7	37.38494454767787	38.0	38.0	38.0	37.0	38.0
8	37.28069356369674	38.0	38.0	38.0	36.0	38.0
9	37.18074025062533	38.0	38.0	38.0	36.0	38.0
10-11	37.134288302605505	38.0	38.0	38.0	35.5	38.0
12-13	37.294645534905435	38.0	38.0	38.0	36.5	38.0
14-15	37.30412514570341	38.0	38.0	38.0	36.5	38.0
16-17	37.24471216426378	38.0	38.0	38.0	36.0	38.0
18-19	37.123848672514384	38.0	38.0	38.0	36.0	38.0
20-21	37.111357600230036	38.0	38.0	38.0	36.0	38.0
22-23	37.090115047722705	38.0	38.0	38.0	36.0	38.0
24-25	37.281822088791735	38.0	38.0	38.0	36.0	38.0
26-27	37.22045042064613	38.0	38.0	38.0	36.5	38.0
28-29	36.77131289208518	38.0	37.0	38.0	35.0	38.0
30-31	37.02932773914684	38.0	38.0	38.0	36.0	38.0
32-33	37.079588845843475	38.0	38.0	38.0	36.0	38.0
34-35	37.16609415918697	38.0	38.0	38.0	36.0	38.0
36-37	37.16906542662886	38.0	38.0	38.0	36.0	38.0
38-39	36.75661887697839	38.0	38.0	38.0	34.5	38.0
40-41	36.9549610272361	38.0	37.5	38.0	35.5	38.0
42-43	37.23197992771256	38.0	38.0	38.0	36.5	38.0
44-45	37.00980425500339	38.0	38.0	38.0	35.5	38.0
46-47	37.14433835965013	38.0	38.0	38.0	36.0	38.0
48-49	36.81033976334983	38.0	37.0	38.0	35.0	38.0
50-51	37.0016804202442	38.0	37.5	38.0	35.5	38.0
52-53	36.92811449737347	38.0	38.0	38.0	35.5	38.0
54-55	36.91818656838707	38.0	37.5	38.0	35.5	38.0
56-57	36.800298981853935	38.0	37.0	38.0	35.0	38.0
58-59	36.92443983415319	38.0	37.5	38.0	35.5	38.0
60-61	36.62301232720426	38.0	37.0	38.0	34.0	38.0
62-63	36.43641456755846	38.0	37.0	38.0	34.0	38.0
64-65	36.78463721783008	38.0	37.0	38.0	34.5	38.0
66-67	36.59090810713877	38.0	37.0	38.0	34.5	38.0
68-69	36.70055436862886	38.0	37.0	38.0	34.0	38.0
70-71	36.23188098852614	38.0	37.0	38.0	32.5	38.0
72-73	35.361783502509034	38.0	37.0	38.0	26.5	38.0
74-75	35.22499389359709	38.0	37.0	38.0	25.0	38.0
76	34.940778713234046	38.0	37.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	2.0
22	6.0
23	17.0
24	54.0
25	105.0
26	237.0
27	410.0
28	808.0
29	1429.0
30	2278.0
31	3624.0
32	5894.0
33	9611.0
34	16488.0
35	31171.0
36	49241.0
37	202055.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.361931630118384	19.27794327360017	11.520674604279382	30.83945049200207
2	18.67971839434068	19.53677909662339	38.90907179583899	22.87443071319694
3	17.131010942055646	22.247403619319112	35.71673710930616	24.904848329319083
4	12.199201684439648	23.426635047351677	37.88072262708275	26.493440641125925
5	11.564754151581019	34.71064925749233	41.24712844470691	12.47746814621975
6	25.651530001762353	43.70391211726767	18.087010830749062	12.557547050220913
7	24.518057947444742	28.948987573856556	23.004906765276058	23.528047713422648
8	21.692416620546577	40.957112954540534	19.146896865173716	18.203573559739173
9	22.05509057573331	16.51758798630929	20.908941938156826	40.51837949980058
10-11	22.616725051092814	28.21776514928996	29.305787014850154	19.859722784767076
12-13	22.406788464927605	22.544066586072454	28.875556146442367	26.17358880255758
14-15	20.77506485154484	23.29476766296366	23.187171297742022	32.742996187749476
16-17	24.840074080715823	28.848038685221887	24.007902767514555	22.303984466547732
18-19	24.597518481530837	25.959941996901964	25.076445980750144	24.36609354081705
20-21	20.1954976486484	25.71444914062041	24.670331539029963	29.419721671701225
22-23	21.016847488335998	29.108372419465052	24.93360253036969	24.94117756182926
24-25	24.51766699234451	29.19768851181716	24.957795861830146	21.32684863400819
26-27	24.21181024700786	29.90143183553834	25.05959540056457	20.827162516889228
28-29	20.681845586848507	29.82691826077278	25.632360534395282	23.858875617983433
30-31	20.400796460450607	29.619455154267833	25.370635467843215	24.609112917438342
32-33	19.86235085690611	34.313346587061844	25.187443380504654	20.63685917552739
34-35	24.923708611728625	26.193531232318485	28.907247604589543	19.97551255136335
36-37	25.254227331331876	28.161957264455168	25.798083671633208	20.785731732579745
38-39	20.464643154181264	26.259542220751875	29.28089762576871	23.994916999298148
40-41	20.373588184187664	24.245202222421476	30.293323769211984	25.08788582417888
42-43	24.125622236650898	24.287635655319544	29.993816281730208	21.592925826299354
44-45	19.980150325726353	28.30959308167739	30.572363193385915	21.13789339921034
46-47	23.885774709288842	24.16419576354771	27.186942500873446	24.76308702629
48-49	22.901020619544816	23.91669320504219	27.240586091005504	25.941700084407493
50-51	20.116394491980095	23.969101653457354	30.592517231889122	25.321986622673425
52-53	18.63537111150975	23.977347939850183	34.971050889813995	22.416230058826077
54-55	18.101992204417495	24.376972096764217	31.837839509991955	25.68319618882633
56-57	22.023891465985674	24.192622817695707	27.825994150102012	25.957491566216607
58-59	18.553515533175798	24.06281316886684	34.19618444331721	23.187486854640156
60-61	22.009897162297996	27.942782030464702	27.22492847753808	22.82239232969922
62-63	17.99802372458329	32.82008934991673	25.79859000944839	23.383296916051595
64-65	17.830355113482625	33.69751264396963	24.99617317947711	23.475959063070643
66-67	17.956291978614587	33.367150782002724	24.61255565573631	24.064001583646377
68-69	18.25035095639483	33.77004471215391	24.040667652024418	23.938936679426842
70-71	18.794722096813096	33.21649669435288	23.78921783874379	24.19956337009023
72-73	18.619822672773438	31.549030947219325	23.612292296130903	26.218854083876337
74-75	18.54325114530845	31.494552150767895	23.51024863591174	26.451948068011916
76	18.801698253816586	31.389865518830145	23.810495653867918	25.997940573485344
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.5
12	5.0
13	4.0
14	9.5
15	18.0
16	33.0
17	46.0
18	73.0
19	142.0
20	231.0
21	278.0
22	336.0
23	553.0
24	893.0
25	1074.0
26	1352.5
27	1703.0
28	2035.0
29	2295.0
30	2557.5
31	3262.0
32	4586.0
33	5468.0
34	6093.0
35	7756.5
36	9562.5
37	10330.0
38	11390.5
39	17248.5
40	24398.5
41	26225.0
42	25699.0
43	24261.5
44	22719.5
45	21948.0
46	21281.0
47	20725.0
48	19272.5
49	17413.0
50	16450.0
51	15373.0
52	12584.0
53	10259.0
54	9646.0
55	8623.0
56	6758.5
57	5127.5
58	4338.0
59	3603.5
60	2497.5
61	1975.5
62	1825.0
63	1635.0
64	1227.5
65	968.5
66	893.0
67	859.0
68	798.5
69	709.5
70	689.0
71	697.0
72	583.5
73	435.0
74	372.0
75	344.0
76	308.0
77	269.5
78	239.0
79	211.0
80	177.0
81	141.0
82	111.5
83	84.0
84	81.0
85	65.5
86	46.0
87	39.0
88	37.0
89	24.0
90	8.0
91	2.5
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.049469593205351374
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	9.275548726003382E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	3.091849575334461E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.01484087796160541
52-53	0.03246442054101184
54-55	0.05287062773821928
56-57	0.054880329962186676
58-59	0.038029749776613865
60-61	0.032773605498545286
62-63	0.029527163444444098
64-65	0.017159765143106256
66-67	0.039111897127980924
68-69	0.008966363768469937
70-71	0.013913323089005072
72-73	0.02287968685747501
74-75	0.014377100525305242
76	0.012367398301337843
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	323431.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.69065735813821
#Duplication Level	Percentage of deduplicated	Percentage of total
1	38.593843395098624	7.985319898216312
2	14.124327555289899	5.844832437212265
3	11.909742976688584	7.392612334624696
4	9.148236700537955	7.571321240079028
5	6.838015540944411	7.074151828365246
6	4.415720263000598	5.481849297067999
7	2.857142857142857	4.138131471627642
8	1.9037656903765692	3.1512130871808828
9	1.2761506276150627	2.3763955836020667
>10	8.407053197848176	33.40186933225325
>50	0.47220561864913335	6.467221756727091
>100	0.04781829049611477	1.478522466924939
>500	0.0014943215780035865	0.15984862304479164
>1k	0.002988643156007173	1.2821900188912008
>5k	0.0	0.0
>10k+	0.0014943215780035865	6.1945206241825925
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT	20035	6.1945206241825925	TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	2600	0.8038808895869599	TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	1547	0.47830912930424113	RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT	517	0.15984862304479164	TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	456	0.14098834063525142	RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT	331	0.10234022094357065	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007420438980802706	0.0
2	0.0	0.0	0.0	0.008347993853403044	0.0
3	0.0	0.0	0.0	0.008347993853403044	0.0
4	0.0	0.0	0.0	0.012058213343804398	0.0
5	0.0	0.0	0.0	0.012367398301337843	0.0
6	0.0	0.0	0.0	0.012367398301337843	0.0
7	0.0	0.0	0.0	0.012367398301337843	0.0
8	0.0	0.0	0.0	0.012985768216404736	0.0
9	0.0	0.0	0.0	0.01576843283420575	0.0
10	0.0	0.0	0.0	0.01886028240954021	0.0
11	0.0	0.0	0.0	0.01886028240954021	0.0
12	0.0	0.0	0.0	0.021024577112274335	0.0
13	0.0	0.0	0.0	0.021024577112274335	0.0
14	0.0	0.0	0.0	0.02133376206980778	0.0
15	0.0	0.0	0.0	0.02195213198487467	0.0
16	0.0	0.0	0.0	0.022261316942408117	0.0
17	0.0	0.0	0.0	0.02442561164514224	0.0
18	0.0	0.0	0.0	0.025662351475276023	0.0
19	0.0	0.0	0.0	0.02999094088074427	0.0
20	0.0	0.0	0.0	0.032155235583478395	0.0
21	0.0	0.0	0.0	0.03370116037114562	0.0
22	0.0	0.0	0.0	0.0355562701163463	0.0
23	0.0	0.0	0.0	0.039266489606747654	0.0
24	0.0	0.0	0.0	0.040503229436881436	0.0
25	0.0	0.0	0.0	0.04081241439441488	0.0
26	0.0	0.0	0.0	0.042358339182082115	0.0
27	0.0	0.0	0.0	0.0516338879080855	0.0
28	0.0	0.0	0.0	0.06523802603955713	0.0
29	0.0	0.0	0.0	0.08286156861896354	0.0
30	0.0	0.0	0.0	0.10821473513670612	0.0
31	0.0	0.0	0.0	0.13913323089005072	0.0
32	0.0	0.0	0.0	0.17190683638859602	0.0
33	0.0	0.0	0.0	0.21086414103781023	0.0
34	0.0	0.0	0.0	0.255077589965093	0.0
35	0.0	0.0	0.0	0.34597796747992615	0.0
36	0.0	0.0	0.0	0.5197399136137228	0.0
37	0.0	0.0	0.0	0.8038808895869598	0.0
38	0.0	0.0	0.0	1.195618230781836	0.0
39	0.0	0.0	0.0	1.6921692725805504	0.0
40	0.0	0.0	0.0	2.2641614440174256	0.0
41	0.0	0.0	0.0	2.8441924243501706	0.0
42	0.0	0.0	0.0	3.4195856303199137	0.0
43	0.0	0.0	0.0	4.097319057233228	0.0
44	0.0	0.0	0.0	4.69064499073991	0.0
45	0.0	0.0	0.0	5.453095096017389	0.0
46	0.0	0.0	0.0	6.204414542823662	0.0
47	0.0	0.0	0.0	7.060547690233775	0.0
48	0.0	0.0	0.0	7.900603219852147	0.0
49	0.0	0.0	0.0	8.750861853069125	0.0
50	0.0	0.0	0.0	9.487031236956259	0.0
51	0.0	0.0	0.0	10.253809931639205	0.0
52	0.0	0.0	0.0	11.022134551109819	0.0
53	0.0	0.0	0.0	11.69151998416973	0.0
54	0.0	0.0	0.0	12.394297392643253	0.0
55	0.0	0.0	0.0	13.030290850289552	0.0
56	0.0	0.0	0.0	13.665047568105717	0.0
57	0.0	0.0	0.0	14.399052657290117	0.0
58	0.0	0.0	0.0	15.077404454118499	0.0
59	0.0	0.0	0.0	15.709378507316861	0.0
60	0.0	0.0	0.0	16.394223188253445	0.0
61	0.0	0.0	0.0	17.063299436355823	0.0
62	0.0	0.0	0.0	17.705167408195255	0.0
63	0.0	0.0	0.0	18.304986225810143	0.0
64	0.0	0.0	0.0	18.889654980505888	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGCCGC	20	0.0069537354	52.488174	19
ACGTGTA	20	0.0069537354	52.488174	18
GCATGTC	20	0.0069537354	52.488174	33
TTTTCCG	20	0.0069537354	52.488174	17
ATCTCGT	2850	0.0	51.93567	37
TCTCGTA	2840	0.0	51.872112	38
GATCTCG	2855	0.0	51.72214	36
CCGTCTT	2995	0.0	51.54819	47
GCCGTCT	3015	0.0	51.438477	46
CGTCTTC	3010	0.0	51.41557	48
TATGCCG	3015	0.0	51.414616	43
CGTATGC	3040	0.0	51.337116	41
TCGTATG	3040	0.0	51.106903	40
CTCGTAT	2995	0.0	50.94011	39
CTGCTTG	3025	0.0	50.829098	54
ATGCCGT	3050	0.0	50.82461	44
TGCCGTC	3070	0.0	50.73716	45
GTATGCC	3060	0.0	50.65852	42
TGATCTC	2905	0.0	50.470554	35
GTCTTCT	3085	0.0	50.067593	49
>>END_MODULE