FastQCFastQC Report
Wed 25 May 2016
SRR1779778_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779778_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51067
Sequences flagged as poor quality0
Sequence length76
%GC53

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTA150.00220497470.0617653
TCGTTGG150.002213572269.9931545
AGTTTAG150.002213572269.9931535
ATGTGTC150.002213572269.9931534
AACGGCT150.002213572269.9931529
CTAGTTA150.002213572269.9931525
TACGAAC150.002213572269.9931523
ACGTGTA150.002213572269.9931534
AACTGTA150.002213572269.9931534
ATAAGGG150.002213572269.9931530
AGACACT150.002213572269.9931567
CATAAGG150.002213572269.9931529
TAAGGGT150.002213572269.9931531
GAACAGA150.002213572269.9931531
TGTACTT150.002213572269.9931537
TCGCGTA150.002213572269.9931534
AGTGTCG150.002213572269.9931535
ATCTGAG150.002213572269.9931511
ACGCGTG150.002213572269.9931532
CTAGTGT207.860195E-569.9931433
Produced by FastQC (version 0.11.5)