##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779778_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	51067
Sequences flagged as poor quality	0
Sequence length	76
%GC	53
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.987702430140796	23.0	12.0	32.0	12.0	34.0
2	23.04848532320285	23.0	12.0	32.0	12.0	34.0
3	22.91511152015979	23.0	11.0	32.0	11.0	34.0
4	22.319247263399063	23.0	12.0	32.0	11.0	34.0
5	22.63087708304776	23.0	11.0	32.0	11.0	34.0
6	23.562163432353575	26.0	12.0	34.0	11.0	38.0
7	24.41868525662365	26.0	12.0	34.0	12.0	38.0
8	23.61497640354828	26.0	11.0	34.0	11.0	38.0
9	25.06419018152623	26.0	12.0	36.0	12.0	38.0
10-11	24.038067636634224	26.0	11.0	35.0	11.0	38.0
12-13	24.6753089079053	26.5	11.0	35.0	11.0	38.0
14-15	23.314792331642742	25.0	11.0	34.0	11.0	38.0
16-17	23.580619578201187	24.5	11.0	34.5	11.0	37.5
18-19	23.980692032036345	26.0	11.5	34.0	11.0	38.0
20-21	23.186930894706954	23.0	11.0	34.0	11.0	37.5
22-23	23.358626901913173	24.5	11.0	32.5	11.0	37.5
24-25	23.198631209979048	23.0	11.0	34.0	11.0	37.5
26-27	22.48641001037852	23.0	11.0	32.5	10.0	37.0
28-29	22.951348228797464	23.0	11.0	34.0	11.0	38.0
30-31	23.40332308535845	24.0	11.0	34.0	11.0	38.0
32-33	23.586269019131727	24.0	11.0	35.0	11.0	38.0
34-35	23.57921945679206	24.5	11.0	35.0	11.0	38.0
36-37	23.341198817240098	24.0	11.0	34.0	11.0	38.0
38-39	23.515792977852627	24.5	11.0	34.0	11.0	37.0
40-41	22.6188536628351	23.0	11.0	34.0	11.0	37.0
42-43	22.95906358313588	23.5	11.0	34.0	11.0	37.5
44-45	23.71782168523704	26.0	11.0	34.5	11.0	38.0
46-47	23.35092133863356	24.5	11.0	35.0	11.0	38.0
48-49	22.519993342080014	22.5	11.0	34.0	11.0	37.5
50-51	22.181731842481447	23.0	11.0	31.0	11.0	37.0
52-53	22.576947931149277	22.5	11.0	34.0	11.0	37.5
54-55	22.497473906828283	23.0	11.0	33.0	11.0	37.5
56-57	22.877445316936573	23.0	11.0	34.0	11.0	38.0
58-59	22.968374880059528	23.0	11.0	34.0	11.0	38.0
60-61	23.513864139268023	23.5	11.0	35.5	11.0	38.0
62-63	23.553655002251944	24.0	11.0	35.5	11.0	38.0
64-65	23.55074705778683	24.0	11.0	35.0	11.0	38.0
66-67	23.073648344331957	24.0	11.0	34.0	11.0	38.0
68-69	22.809260383417865	23.5	11.0	34.0	11.0	37.5
70-71	22.782922435232145	23.5	11.0	34.0	11.0	37.5
72-73	22.586337556543363	23.0	11.0	33.0	11.0	37.0
74-75	22.198993479154836	22.5	11.0	33.5	11.0	37.0
76	21.734799381205082	21.0	11.0	33.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
3	1.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	14.0
12	67.0
13	399.0
14	1099.0
15	1819.0
16	2932.0
17	4486.0
18	5733.0
19	5860.0
20	4881.0
21	3589.0
22	2390.0
23	1688.0
24	1277.0
25	936.0
26	805.0
27	698.0
28	729.0
29	735.0
30	788.0
31	920.0
32	1102.0
33	1330.0
34	1714.0
35	2087.0
36	2038.0
37	950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.798147531674076	15.996631875771044	18.544265376857854	41.66095521569703
2	14.234241290853191	11.527992637123779	52.607358959797914	21.630407112225118
3	12.691170423169563	12.671588305559364	52.64456498325729	21.992676288013786
4	12.385931931226256	24.715074609329104	25.026436376454004	37.87255708299064
5	14.432020678716196	19.012277987741594	45.685080384592794	20.87062094894942
6	19.121937846358705	36.9397066598782	15.330839877024301	28.607515616738795
7	18.68134020012924	20.408482973348736	17.25184561458476	43.65833121193726
8	16.05373438295539	37.069674538832096	15.154897583519366	31.72169349469314
9	16.08310813457095	16.27697489523362	15.93428112638546	51.70563584380997
10-11	17.80049347902714	22.983981514118984	29.283672110601966	29.93185289625191
12-13	16.020444131124428	14.927740571025733	35.77820859280148	33.27360670504837
14-15	24.23824070810324	21.716993694434652	21.99604433478244	32.04872126267967
16-17	29.791838013551093	15.5367563545216	21.08447890964634	33.58692672228097
18-19	17.422551208240318	14.735832060470763	31.41033172756825	36.431285003720674
20-21	29.797712763874202	14.698625308424393	22.74703325108683	32.75662867661458
22-23	32.38162378098931	13.191751850546352	20.64485176046685	33.781772607997496
24-25	18.41930051306153	23.36192378490581	19.484588571652374	38.73418713038029
26-27	28.366231935142753	22.20557709630674	21.333176673324715	28.095014295225784
28-29	20.554772255512475	21.707202443896133	23.955273567540047	33.78275173305135
30-31	19.122312301727177	20.80738651940626	26.525476833901223	33.54482434496534
32-33	21.036501782007598	18.94606979203384	26.40210707711589	33.61532134884268
34-35	20.24341048838758	20.19837073591039	25.55810128069557	34.00011749500646
36-37	28.712842204206325	13.386597736262877	31.835272000939963	26.06528805859084
38-39	28.464144440527946	10.695962088277915	38.211334351623385	22.628559119570753
40-41	23.41479653781381	23.692868053107745	23.54502016997611	29.34731523910234
42-43	14.947323072102769	24.64066110523636	28.630595699682765	31.781420122978105
44-45	15.524027728821524	15.488779226882857	33.85422786198254	35.132965182313086
46-47	27.710218149061998	14.736811185524616	26.359025574746408	31.19394509066698
48-49	26.18865781537618	15.274350840089296	31.098969960443345	27.438021384091176
50-51	41.487486781811775	12.59742294285826	23.569498296322404	22.34559197900756
52-53	15.057964203188032	18.709121529001685	31.965495633102258	34.26741863470802
54-55	26.258175694199664	15.79230799357694	25.069517878823483	32.87999843339991
56-57	25.9326348771174	16.651326740428864	23.5797512973661	33.83628708508763
58-59	16.066463008655465	20.380487995926842	30.1090745309991	33.443974464418595
60-61	15.778600242823012	17.81518035483492	29.672384756981163	36.73383464536091
62-63	16.103669760701838	20.749618141229	29.040849097246703	34.10586300082247
64-65	15.9803000039165	22.76367837700231	27.82183840520111	33.434183213880075
66-67	13.519758743586733	34.55430227548663	18.47217326596953	33.453765714957115
68-69	14.689896289332196	33.183496391182146	18.629726474131093	33.49688084535457
70-71	13.473739866055693	36.27364587005052	17.333450828339796	32.91916343555399
72-73	12.130380292170916	39.12387890181334	16.45909215525007	32.28664865076568
74-75	15.5367563545216	26.946500607057533	21.273450045039752	36.24329299338112
76	17.82595073042729	23.824070810323896	23.947440567109233	34.402537892139584
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	0.5
17	0.0
18	0.5
19	2.5
20	8.5
21	13.0
22	13.0
23	27.0
24	49.0
25	57.0
26	63.0
27	79.5
28	128.5
29	167.0
30	193.0
31	284.0
32	407.5
33	466.0
34	528.5
35	693.5
36	900.0
37	1004.0
38	1099.0
39	1331.5
40	1558.0
41	1763.0
42	1879.0
43	1923.0
44	2128.0
45	2296.0
46	2303.0
47	2329.0
48	2364.0
49	2325.5
50	2278.0
51	2214.0
52	2091.5
53	1968.0
54	1903.0
55	1857.5
56	1779.0
57	1681.5
58	1617.0
59	1556.0
60	1500.5
61	1477.0
62	1448.0
63	1416.5
64	1335.5
65	1249.0
66	1209.0
67	1206.0
68	1176.5
69	1045.5
70	902.5
71	861.0
72	849.5
73	751.0
74	638.5
75	613.0
76	523.0
77	402.5
78	316.5
79	261.0
80	233.5
81	168.5
82	105.0
83	79.0
84	63.0
85	30.5
86	10.5
87	7.0
88	8.0
89	5.5
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0019582117610198366
5	0.0
6	0.0
7	0.0
8	0.0019582117610198366
9	0.0019582117610198366
10-11	0.0019582117610198366
12-13	0.0019582117610198366
14-15	0.0019582117610198366
16-17	0.0019582117610198366
18-19	0.0019582117610198366
20-21	0.0019582117610198366
22-23	0.0019582117610198366
24-25	0.0019582117610198366
26-27	0.0019582117610198366
28-29	0.0019582117610198366
30-31	0.0019582117610198366
32-33	0.0019582117610198366
34-35	0.0019582117610198366
36-37	0.0019582117610198366
38-39	0.0019582117610198366
40-41	0.0019582117610198366
42-43	0.0019582117610198366
44-45	0.0019582117610198366
46-47	0.0019582117610198366
48-49	0.0019582117610198366
50-51	0.0019582117610198366
52-53	0.0019582117610198366
54-55	0.0019582117610198366
56-57	0.003916423522039673
58-59	0.0019582117610198366
60-61	0.0019582117610198366
62-63	0.0019582117610198366
64-65	0.0019582117610198366
66-67	0.0019582117610198366
68-69	0.022519435251728122
70-71	0.0019582117610198366
72-73	0.0019582117610198366
74-75	0.0019582117610198366
76	0.0019582117610198366
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	51067.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.44974249515343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.51364549285236	98.96606419018153
2	0.42925215610601347	0.8537803278046489
3	0.051195211278698846	0.15274051735954725
4	0.0039380931752845275	0.015665694088158693
5	0.0	0.0
6	0.0019690465876422637	0.01174927056611902
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.003916423522039673	0.0
2	0.0	0.0	0.0	0.003916423522039673	0.0
3	0.0	0.0	0.0	0.003916423522039673	0.0
4	0.0	0.0	0.0	0.013707482327138856	0.0
5	0.0	0.0	0.0	0.015665694088158693	0.0
6	0.0	0.0	0.0	0.017623905849178532	0.0
7	0.0	0.0	0.0	0.017623905849178532	0.0
8	0.0	0.0	0.0	0.021540329371218202	0.0
9	0.0	0.0	0.0	0.025456752893257877	0.0
10	0.0	0.0	0.0	0.031331388176317386	0.0
11	0.0	0.0	0.0	0.031331388176317386	0.0
12	0.0	0.0	0.0	0.031331388176317386	0.0
13	0.0	0.0	0.0	0.031331388176317386	0.0
14	0.0	0.0	0.0	0.031331388176317386	0.0
15	0.0	0.0	0.0	0.031331388176317386	0.0
16	0.0	0.0	0.0	0.031331388176317386	0.0
17	0.0	0.0	0.0	0.031331388176317386	0.0
18	0.0	0.0	0.0	0.033289599937337225	0.0
19	0.0	0.0	0.0	0.048955294025495914	0.0
20	0.0	0.0	0.0	0.05091350578651575	0.0
21	0.0	0.0	0.0	0.06070456459161494	0.0
22	0.0	0.0	0.0	0.09399416452895216	0.0
23	0.0	0.0	0.0	0.1116180703781307	0.0
24	0.0	0.0	0.0	0.1174927056611902	0.0
25	0.0	0.0	0.0	0.11945091742221003	0.0
26	0.0	0.0	0.0	0.11945091742221003	0.0
27	0.0	0.0	0.0	0.14490767031546792	0.0
28	0.0	0.0	0.0	0.18407190553586464	0.0
29	0.0	0.0	0.0	0.21736150547320188	0.0
30	0.0	0.0	0.0	0.2624003759766581	0.0
31	0.0	0.0	0.0	0.30156461119705485	0.0
32	0.0	0.0	0.0	0.36031096402764995	0.0
33	0.0	0.0	0.0	0.407308046292126	0.0
34	0.0	0.0	0.0	0.4621379756006815	0.0
35	0.0	0.0	0.0	0.6618755752247047	0.0
36	0.0	0.0019582117610198366	0.0	0.7832847044079346	0.0
37	0.0	0.0019582117610198366	0.0	1.0300193862964342	0.0
38	0.0	0.0019582117610198366	0.0	1.2434644682475964	0.0
39	0.0	0.0019582117610198366	0.0	1.4804080913309965	0.0
40	0.0	0.0019582117610198366	0.0	1.750641314351734	0.0
41	0.0	0.0019582117610198366	0.0	1.968002819824936	0.0
42	0.0	0.0019582117610198366	0.0	2.15794936064386	0.0
43	0.0	0.0019582117610198366	0.0	2.3988094072493	0.0
44	0.0	0.0019582117610198366	0.0	2.6435858773767795	0.0
45	0.0	0.0019582117610198366	0.0	2.8354906299567237	0.0
46	0.0	0.0019582117610198366	0.0	3.058726770712985	0.0
47	0.0	0.0019582117610198366	0.0	3.2682554291421075	0.0
48	0.0	0.0019582117610198366	0.0	3.3916227700863573	0.0
49	0.0	0.0019582117610198366	0.0	3.548279710967944	0.0
50	0.0	0.0019582117610198366	0.0	3.751933734114007	0.0
51	0.0	0.0019582117610198366	0.0	4.0025848395245465	0.0
52	0.0	0.0019582117610198366	0.0	4.172949262733272	0.0
53	0.0	0.0019582117610198366	0.0	4.366812227074236	0.0
54	0.0	0.0019582117610198366	0.0	4.550884132610101	0.0
55	0.0	0.0019582117610198366	0.0	4.725164979340866	0.0
56	0.0	0.0019582117610198366	0.0	4.864198014373274	0.0
57	0.0	0.0019582117610198366	0.0	4.985607143556504	0.0
58	0.0	0.0019582117610198366	0.0	5.136389449155032	0.0
59	0.0	0.0019582117610198366	0.0	5.302837448841718	0.0
60	0.0	0.0019582117610198366	0.0	5.406622672175769	0.0
61	0.0	0.0019582117610198366	0.0	5.506491471987781	0.0
62	0.0	0.0019582117610198366	0.0	5.592652789472654	0.0
63	0.0	0.0019582117610198366	0.0	5.6748976834354865	0.0
64	0.0	0.0019582117610198366	0.0	5.731685824505062	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGTA	15	0.002204974	70.06176	53
TCGTTGG	15	0.0022135722	69.99315	45
AGTTTAG	15	0.0022135722	69.99315	35
ATGTGTC	15	0.0022135722	69.99315	34
AACGGCT	15	0.0022135722	69.99315	29
CTAGTTA	15	0.0022135722	69.99315	25
TACGAAC	15	0.0022135722	69.99315	23
ACGTGTA	15	0.0022135722	69.99315	34
AACTGTA	15	0.0022135722	69.99315	34
ATAAGGG	15	0.0022135722	69.99315	30
AGACACT	15	0.0022135722	69.99315	67
CATAAGG	15	0.0022135722	69.99315	29
TAAGGGT	15	0.0022135722	69.99315	31
GAACAGA	15	0.0022135722	69.99315	31
TGTACTT	15	0.0022135722	69.99315	37
TCGCGTA	15	0.0022135722	69.99315	34
AGTGTCG	15	0.0022135722	69.99315	35
ATCTGAG	15	0.0022135722	69.99315	11
ACGCGTG	15	0.0022135722	69.99315	32
CTAGTGT	20	7.860195E-5	69.99314	33
>>END_MODULE