FastQCFastQC Report
Wed 25 May 2016
SRR1779778_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779778_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51067
Sequences flagged as poor quality0
Sequence length76
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT2484248.64589656725478No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG29715.817847141989935No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC17033.334834629016782No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT10792.1129104901404037RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC7341.43732743258856No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC5861.1475120919576243No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC4350.851822116043629RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT3920.767619010319776No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG2510.491511152015979No Hit
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC2480.4856365167329195No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT2290.4484304932735426RNA PCR Primer, Index 48 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG2220.43472301094640375No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC1950.38185129339886814No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCG1310.2565257406935986No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCATCTCGTATGCCGTC1270.24869289364951924No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT1100.21540329371218203No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT990.19386296434096384No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAGCTCGTATGCCGT920.18015548201382497No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG840.1644897879256663No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGCCAATCTCGTATGCCGT710.1390330350324084No Hit
TCTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT700.13707482327138856No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCC150.002212712870.070
CCAATCT150.002212712870.034
CTTTAGT207.8563826E-570.028
CGCCAAT150.002212712870.032
TAGTCAA150.002212712870.031
TCTATAC207.8563826E-570.02
ACTTTAG207.8563826E-570.027
AAAATGT150.002212712870.070
TGAACAA150.002212712870.059
TCTTTAT401.3096724E-1070.01
GACTTTA207.8563826E-570.026
AATAACA150.002212712870.069
TTAGTCA150.002212712870.030
AGTCAAT150.002212712870.032
CTTAATA401.04028E-861.251
TAATACA401.04028E-861.253
TTAATAC401.04028E-861.252
AAATAAC252.369384E-455.99999668
CTCTATA252.369384E-455.9999961
CTTTACA452.6357156E-854.4444431