FastQCFastQC Report
Wed 25 May 2016
SRR1779777_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779777_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56184
Sequences flagged as poor quality0
Sequence length76
%GC54

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTGTA353.6288839E-970.0000134
CTATGTA150.002213870270.026
GTACCGA150.002213870270.038
CTCCGTA150.002213870270.053
TAAACTA150.002213870270.039
CACGAAA150.002213870270.023
TATGATG150.002213870270.011
AGAAACA150.002213870270.067
ATCTTGG150.002213870270.042
GGAGTTT150.002213870270.033
TAGATAT150.002213870270.039
CGACTTG150.002213870270.025
CGACTAT252.8059349E-670.022
CGACTAG207.863229E-570.022
CGTTTAT207.863229E-570.035
ACCTTGG150.002213870270.042
CCGCAAT150.002213870270.024
GACTAGG150.002213870270.023
GCCGTAT950.062.63158454
GTATATC352.9249168E-760.00000438
Produced by FastQC (version 0.11.5)