##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779777_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	56184
Sequences flagged as poor quality	0
Sequence length	76
%GC	54
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.546792681190375	23.0	12.0	34.0	12.0	34.0
2	23.89707034031041	23.0	12.0	34.0	12.0	34.0
3	23.741136266552754	23.0	11.0	33.0	11.0	34.0
4	23.729976505766768	23.0	12.0	34.0	11.0	34.0
5	23.93448312686886	23.0	11.0	34.0	11.0	34.0
6	25.078385305425034	27.0	12.0	36.0	11.0	38.0
7	26.06090701979211	27.0	12.0	37.0	12.0	38.0
8	25.289246048697137	27.0	11.0	36.0	11.0	38.0
9	26.979211163320517	31.0	12.0	37.0	12.0	38.0
10-11	25.842241563434428	27.5	11.0	37.0	11.0	38.0
12-13	26.194441478000854	29.5	11.0	36.5	11.0	38.0
14-15	25.16327602164317	26.5	11.0	36.5	11.0	38.0
16-17	25.259646874555035	27.0	11.0	36.0	11.0	38.0
18-19	25.673955218567563	27.0	11.5	36.5	11.0	38.0
20-21	24.780987469742275	26.5	11.0	35.0	11.0	38.0
22-23	24.94898013669372	26.5	11.0	35.5	11.0	38.0
24-25	25.059616616830414	26.5	11.0	36.5	11.0	38.0
26-27	24.19359604157767	24.5	11.0	35.0	10.0	38.0
28-29	24.993129716645306	26.0	11.0	37.0	11.0	38.0
30-31	25.503889007546633	27.0	11.0	37.0	11.0	38.0
32-33	25.660170155204327	27.0	11.0	37.0	11.0	38.0
34-35	25.68370888509184	27.0	11.0	37.0	11.0	38.0
36-37	24.92189947315962	26.5	11.0	35.5	11.0	38.0
38-39	24.6138135412217	25.5	11.0	34.0	11.0	37.5
40-41	24.163151431012388	26.0	11.0	34.5	11.0	38.0
42-43	24.693631638900754	26.0	11.0	35.5	11.0	38.0
44-45	25.713530542503204	27.0	11.0	37.0	11.0	38.0
46-47	25.11295920546775	26.5	11.0	36.0	11.0	38.0
48-49	24.37306884522284	25.5	11.0	35.0	11.0	38.0
50-51	23.487540936921544	23.5	11.0	34.0	11.0	37.5
52-53	24.595089349280933	25.5	11.0	36.5	11.0	38.0
54-55	24.33605652854905	24.5	11.0	35.5	11.0	38.0
56-57	24.70926776306422	25.0	11.0	36.5	11.0	38.0
58-59	25.038623095543215	26.0	11.0	37.0	11.0	38.0
60-61	25.67432899045992	27.0	11.0	37.0	11.0	38.0
62-63	25.631852484693148	27.0	11.0	37.0	11.0	38.0
64-65	25.53002634201908	27.0	11.0	37.0	11.0	38.0
66-67	24.649829132849213	25.0	11.0	36.0	11.0	38.0
68-69	24.42930371636053	25.0	11.0	35.5	11.0	38.0
70-71	24.347616759219708	25.0	11.0	35.0	11.0	38.0
72-73	23.886951801224548	25.0	11.0	34.0	11.0	37.5
74-75	24.04664139256728	24.5	11.0	35.0	11.0	38.0
76	23.702157197778728	24.0	11.0	35.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
5	1.0
6	0.0
7	0.0
8	0.0
9	1.0
10	0.0
11	8.0
12	77.0
13	335.0
14	866.0
15	1541.0
16	2546.0
17	3945.0
18	5108.0
19	5276.0
20	4621.0
21	3421.0
22	2480.0
23	1685.0
24	1415.0
25	1181.0
26	1081.0
27	976.0
28	1044.0
29	1098.0
30	1315.0
31	1427.0
32	1782.0
33	2165.0
34	2667.0
35	3224.0
36	3323.0
37	1575.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.22511747116617	19.722696853196638	12.905809483126868	39.146376192510324
2	14.728392424889647	13.235084721628935	47.380037021216005	24.656485832265414
3	16.339171294318668	11.553111206037306	47.32486117043998	24.782856329204044
4	13.81354122170013	23.083084152071763	26.238786843229388	36.86458778299872
5	15.089705254164887	20.085789548625943	43.179552897622095	21.644952299587068
6	23.059945892068917	32.14438274241777	14.724832692581519	30.070838672931792
7	22.097038302719636	18.00512601452371	19.058806777730315	40.83902890502634
8	18.637641949378807	32.07788971556727	15.796874443772028	33.487593891281904
9	18.548315326700248	13.733691686097218	16.35370129754552	51.364291689657016
10-11	20.576692030436526	20.36666221688248	28.18226316023673	30.874382592444267
12-13	18.983500347080078	13.903671929231262	33.43627075805849	33.67655696563017
14-15	24.76416773458636	19.88288480706251	21.80869998576107	33.54424747259006
16-17	29.739952297889005	15.32786301662454	21.426791499056637	33.505393186429814
18-19	19.82200863258132	14.564143639033505	29.86250166866907	35.751346059716106
20-21	29.521648200062295	14.621100876607485	23.130868152894585	32.726382770435634
22-23	31.567049944822184	13.320992488697447	21.428571428571427	33.68338613790894
24-25	20.8344086787046	21.454697553552197	19.466569366451004	38.2443244012922
26-27	28.357717499644025	20.942795101808347	21.76687313114054	28.932614267407093
28-29	22.696569216393005	20.733324433764963	23.276821074177903	33.29328527566413
30-31	21.792179128918	19.49344107648221	25.05562180730826	33.65875798729153
32-33	22.99094032002563	18.334727586636525	25.39736219141021	33.27696990192763
34-35	22.303009807237064	19.802253350657672	24.90433049142979	32.99040635067547
36-37	28.70530231564708	13.86540412580318	30.54482672694587	26.884466831603866
38-39	28.09084760243494	11.806272471610125	36.368409810971485	23.73447011498345
40-41	24.190132070769998	22.185931437115087	23.450571357374248	30.173365134740664
42-43	17.363638790748173	22.863474151663745	27.73056146377495	32.04232559381313
44-45	17.929070440083656	15.579584390157079	32.22355715747786	34.2677880122814
46-47	27.354359859744047	14.880835840022783	26.708256946051296	31.05654735418187
48-49	26.468860687396546	15.477101614367337	30.418453980741507	27.635583717494615
50-51	37.8285053174921	13.528233880656787	24.969518978329553	23.67374182352156
52-53	17.161343146765397	18.794443010599572	30.285770226854037	33.758443615781
54-55	25.65679399095796	16.005126197002596	25.840126730981456	32.49795308105799
56-57	26.023886228930458	16.998914262321343	24.098036772689248	32.879162736058944
58-59	17.83651492902594	20.33818359809549	28.66372980910426	33.16157166377431
60-61	17.73223216987345	17.64946692059876	28.686613388391507	35.93168752113628
62-63	17.87211320250968	20.349753036977706	27.96066390780047	33.817469852712144
64-65	18.22364615316157	21.604592177279404	26.856227472967557	33.315534196591464
66-67	15.895801998878634	31.625179107001415	18.461593229087867	34.017425665032086
68-69	16.66963395527703	30.191568152684802	18.886198547215496	34.25259934482268
70-71	15.542344523156885	33.419066604962445	17.49670713039764	33.54188174148304
72-73	14.627200398697116	35.48582311375327	16.6242457682929	33.262730719256716
74-75	17.66402050478801	25.47168132141967	20.949770389092592	35.91452778469972
76	19.65754156135417	22.86675447652273	23.3597949521199	34.1159090100032
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	0.5
15	0.0
16	2.0
17	4.0
18	5.0
19	7.5
20	11.0
21	13.0
22	15.5
23	26.0
24	48.5
25	63.0
26	73.5
27	95.5
28	136.5
29	166.0
30	188.5
31	264.0
32	367.0
33	417.0
34	481.0
35	647.0
36	821.0
37	893.0
38	1002.5
39	1212.5
40	1406.5
41	1605.5
42	1711.0
43	1855.0
44	2069.0
45	2214.0
46	2289.0
47	2326.5
48	2398.5
49	2354.0
50	2275.0
51	2250.5
52	2193.0
53	2123.0
54	2086.0
55	2031.0
56	2028.5
57	2033.0
58	1985.0
59	1926.5
60	1849.0
61	1814.5
62	1799.0
63	1792.0
64	1733.0
65	1644.5
66	1564.5
67	1521.0
68	1447.5
69	1332.5
70	1235.5
71	1180.0
72	1126.0
73	1029.5
74	854.0
75	721.0
76	652.5
77	537.5
78	427.5
79	364.0
80	320.0
81	230.5
82	147.0
83	109.0
84	91.5
85	61.5
86	37.0
87	25.0
88	18.5
89	8.5
90	4.0
91	3.0
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.003559732308130429
9	0.0017798661540652145
10-11	0.0026697992310978215
12-13	0.0017798661540652145
14-15	0.0
16-17	0.003559732308130429
18-19	0.0026697992310978215
20-21	0.0026697992310978215
22-23	0.003559732308130429
24-25	8.899330770326073E-4
26-27	0.0
28-29	0.0026697992310978215
30-31	0.0017798661540652145
32-33	0.0017798661540652145
34-35	0.0017798661540652145
36-37	0.0017798661540652145
38-39	0.003559732308130429
40-41	0.003559732308130429
42-43	8.899330770326073E-4
44-45	0.0026697992310978215
46-47	0.0017798661540652145
48-49	0.0017798661540652145
50-51	0.0026697992310978215
52-53	0.004449665385163036
54-55	0.003559732308130429
56-57	0.0017798661540652145
58-59	0.0026697992310978215
60-61	0.0017798661540652145
62-63	0.0026697992310978215
64-65	0.0026697992310978215
66-67	0.004449665385163036
68-69	0.028477858465043433
70-71	0.003559732308130429
72-73	0.0017798661540652145
74-75	0.003559732308130429
76	0.003559732308130429
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	56184.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.11184678912146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.19367872856245	98.31268688594618
2	0.7308970099667774	1.4488110494090845
3	0.06464936697494837	0.19222554463904315
4	0.007183262997216486	0.028477858465043433
5	0.003591631498608243	0.017798661540652145
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0017798661540652143	0.0
6	0.0	0.0	0.0	0.0017798661540652143	0.0
7	0.0	0.0	0.0	0.005339598462195643	0.0
8	0.0	0.0	0.0	0.007119464616260857	0.0
9	0.0	0.0	0.0	0.0124590630784565	0.0
10	0.0	0.0	0.0	0.01779866154065214	0.0
11	0.0	0.0	0.0	0.021358393848782572	0.0
12	0.0	0.0	0.0	0.021358393848782572	0.0
13	0.0	0.0	0.0	0.023138260002847785	0.0
14	0.0	0.0	0.0	0.024918126156913	0.0
15	0.0	0.0	0.0	0.024918126156913	0.0
16	0.0	0.0	0.0	0.024918126156913	0.0
17	0.0	0.0	0.0	0.024918126156913	0.0
18	0.0	0.0	0.0	0.024918126156913	0.0
19	0.0	0.0	0.0	0.024918126156913	0.0
20	0.0	0.0	0.0	0.03915705538943472	0.0
21	0.0	0.0	0.0	0.042716787697565144	0.0
22	0.0	0.0	0.0	0.05695571693008686	0.0
23	0.0	0.0	0.0	0.060515449238217285	0.0
24	0.0	0.0	0.0	0.06585504770041294	0.0
25	0.0	0.0	0.0	0.06941478000854336	0.0
26	0.0	0.0	0.0	0.07297451231667379	0.0
27	0.0	0.0	0.0	0.10323223693578243	0.0
28	0.0	0.0	0.0	0.15306848924960842	0.0
29	0.0	0.0	0.0	0.19222554463904315	0.0
30	0.0	0.0	0.0	0.24206179695286914	0.0
31	0.0	0.0	0.0	0.2705396554179126	0.0
32	0.0	0.0	0.0	0.323935640039869	0.0
33	0.0	0.0	0.0	0.37911149081589063	0.0
34	0.0	0.0	0.0	0.46632493236508615	0.0
35	0.0	0.0	0.0	0.6015947600740424	0.0
36	0.0	0.0	0.0	0.7208457923964118	0.0
37	0.0	0.0	0.0	1.0358821016659547	0.0
38	0.0	0.0	0.0	1.377616403246476	0.0
39	0.0	0.0	0.0	1.6890929802078885	0.0
40	0.0	0.0	0.0	2.000569557169301	0.0
41	0.0	0.0	0.0	2.278228677203474	0.0
42	0.0	0.0	0.0	2.5808059233945606	0.0
43	0.0	0.0	0.0	2.8994019649722342	0.0
44	0.0	0.0	0.0	3.2518154634771466	0.0
45	0.0	0.0	0.0	3.5081161896625375	0.0
46	0.0	0.0	0.0	3.814253168161754	0.0
47	0.0	0.0	0.0	4.120390146660971	0.0
48	0.0	0.0	0.0	4.305496226683753	0.0
49	0.0	0.0	0.0	4.455004983625232	0.0
50	0.0	0.0	0.0	4.721984906735013	0.0
51	0.0	0.0	0.0	5.047700412928948	0.0
52	0.0	0.0	0.0	5.305781005268404	0.0
53	0.0	0.0	0.0	5.606578385305425	0.0
54	0.0	0.0	0.0	5.921614694574968	0.0
55	0.0	0.0	0.0	6.136978499216859	0.0
56	0.0	0.0	0.0	6.350562437704685	0.0
57	0.0	0.0	0.0	6.508970525416489	0.0
58	0.0	0.0	0.0	6.708315534671793	0.0
59	0.0	0.0	0.0	6.893421614694575	0.0
60	0.0	0.0	0.0	7.048269970098248	0.0
61	0.0	0.0	0.0	7.213797522426313	0.0
62	0.0	0.0	0.0	7.30813042859177	0.0
63	0.0	0.0	0.0	7.423821728606009	0.0
64	0.0	0.0	0.0	7.525274099387726	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTGTA	35	3.6288839E-9	70.00001	34
CTATGTA	15	0.0022138702	70.0	26
GTACCGA	15	0.0022138702	70.0	38
CTCCGTA	15	0.0022138702	70.0	53
TAAACTA	15	0.0022138702	70.0	39
CACGAAA	15	0.0022138702	70.0	23
TATGATG	15	0.0022138702	70.0	11
AGAAACA	15	0.0022138702	70.0	67
ATCTTGG	15	0.0022138702	70.0	42
GGAGTTT	15	0.0022138702	70.0	33
TAGATAT	15	0.0022138702	70.0	39
CGACTTG	15	0.0022138702	70.0	25
CGACTAT	25	2.8059349E-6	70.0	22
CGACTAG	20	7.863229E-5	70.0	22
CGTTTAT	20	7.863229E-5	70.0	35
ACCTTGG	15	0.0022138702	70.0	42
CCGCAAT	15	0.0022138702	70.0	24
GACTAGG	15	0.0022138702	70.0	23
GCCGTAT	95	0.0	62.631584	54
GTATATC	35	2.9249168E-7	60.000004	38
>>END_MODULE