FastQCFastQC Report
Wed 25 May 2016
SRR1779777_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779777_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56184
Sequences flagged as poor quality0
Sequence length76
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT2196639.09653994019649No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG26424.702406379040296No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC15532.7641321372632777No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT9381.669514452513171TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC7061.2565855047700412No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC5841.0394418339740852No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT4610.8205182970240638No Hit
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC4450.7920404385590203No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG2360.4200484123593906No Hit
TTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC2290.4075893492809341No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC2180.38801082158621675No Hit
TATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCT2060.3666524277374341No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGT2000.3559732308130429No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG1830.3257155061939342No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCG1700.30257724619108645No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAGCTCGTATGCCGT1050.1868859461768475No Hit
CCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG910.1619678200199345No Hit
TCTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT800.14238929232521716No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGCATCTCGTATGCCGT760.1352698277089563No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGGATCTCGTATGCCGT710.12637049693863023No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAACT150.002213870270.070
AAATTTT150.002213870270.069
AAATCCC150.002213870270.070
CACGCAT150.002213870270.032
ACACAAA150.002213870270.070
CCTTATA207.863229E-570.01
CGCATCT150.002213870270.034
TCTTAAT207.863229E-570.01
TCTTTAT650.064.615391
CTTTACA352.9249168E-760.0000041
ACAACAC252.3714367E-455.99999670
AAATCCG200.00691782252.568
ACGCATC200.00691782252.533
GCACGCA200.00691782252.531
AATACAA200.00691782252.569
GCATCTC200.00691782252.535
TTTACAC407.356466E-752.52
GAGACGT200.00691782252.524
ACGTGCA200.00691782252.527
TCTCGTA33550.052.05663338
Produced by FastQC (version 0.11.5)