##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779777_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 56184 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.65550690588068 34.0 34.0 34.0 34.0 34.0 2 33.665812330912715 34.0 34.0 34.0 34.0 34.0 3 33.69699558593194 34.0 34.0 34.0 34.0 34.0 4 33.71614694574968 34.0 34.0 34.0 34.0 34.0 5 33.74620888509184 34.0 34.0 34.0 34.0 34.0 6 37.443346860316105 38.0 38.0 38.0 37.0 38.0 7 37.41122027623523 38.0 38.0 38.0 37.0 38.0 8 37.312437704684605 38.0 38.0 38.0 36.0 38.0 9 37.339972234087995 38.0 38.0 38.0 37.0 38.0 10-11 37.229478143243625 38.0 38.0 38.0 36.0 38.0 12-13 37.20655346717926 38.0 38.0 38.0 36.0 38.0 14-15 37.229691727182114 38.0 38.0 38.0 36.0 38.0 16-17 37.09715399401965 38.0 38.0 38.0 35.5 38.0 18-19 37.047976292182824 38.0 38.0 38.0 35.0 38.0 20-21 37.09787483981204 38.0 38.0 38.0 35.5 38.0 22-23 37.112487540936925 38.0 38.0 38.0 36.0 38.0 24-25 36.958591413925674 38.0 38.0 38.0 34.5 38.0 26-27 36.803226897337325 38.0 38.0 38.0 34.5 38.0 28-29 36.76198739854763 38.0 37.5 38.0 34.0 38.0 30-31 36.15722447671935 38.0 37.0 38.0 33.5 38.0 32-33 36.24899437562296 38.0 37.0 38.0 34.0 38.0 34-35 36.357174640467036 38.0 37.0 38.0 34.0 38.0 36-37 36.12415456357682 38.0 37.0 38.0 34.0 38.0 38-39 34.436912644169155 37.0 34.5 38.0 25.5 38.0 40-41 36.073873344724475 38.0 36.5 38.0 33.0 38.0 42-43 36.68410045564573 38.0 37.0 38.0 34.0 38.0 44-45 36.690063007261855 38.0 37.0 38.0 34.0 38.0 46-47 36.68535526128436 38.0 37.0 38.0 34.0 38.0 48-49 36.62924498077744 38.0 37.0 38.0 34.0 38.0 50-51 36.3973729175566 38.0 37.0 38.0 34.0 38.0 52-53 36.409636195358104 38.0 37.0 38.0 34.0 38.0 54-55 36.3930834401253 38.0 37.0 38.0 34.0 38.0 56-57 36.3928164602022 38.0 37.0 38.0 34.0 38.0 58-59 36.177888722768046 38.0 37.0 38.0 34.0 38.0 60-61 36.17700768902179 38.0 37.0 38.0 34.0 38.0 62-63 36.40483945607291 38.0 37.0 38.0 34.0 38.0 64-65 36.52757902605724 38.0 37.0 38.0 34.0 38.0 66-67 36.35296525701267 38.0 37.0 38.0 34.0 38.0 68-69 35.793597821443825 38.0 37.0 38.0 31.0 38.0 70-71 32.21015769614125 36.0 29.5 38.0 18.5 38.0 72-73 25.939128577530973 26.0 15.0 38.0 10.0 38.0 74-75 25.166052612843515 24.5 11.0 38.0 11.0 38.0 76 25.002100242061797 25.0 11.0 38.0 11.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 1.0 20 0.0 21 2.0 22 8.0 23 13.0 24 28.0 25 78.0 26 134.0 27 224.0 28 388.0 29 657.0 30 951.0 31 1417.0 32 2154.0 33 3272.0 34 5931.0 35 13957.0 36 12412.0 37 14556.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.79567136551331 7.584009682471877 12.008756941478001 60.611562010536815 2 11.022711092125872 7.0909867577958146 61.640324647586496 20.24597750249181 3 11.115264132137263 9.35675637192083 61.16332051829703 18.364658977644883 4 9.098675779581376 48.78791114908159 22.305282642745265 19.80813042859177 5 9.84799943044283 21.51146233803218 57.98625943329062 10.654278798234373 6 17.298519151359816 57.50925530400114 11.764915278371065 13.427310266267977 7 15.484835540367364 19.39520148084864 10.563505624377047 54.55645735440695 8 13.302719635483411 58.813897194930945 8.123309127153638 19.76007404243201 9 11.423180976790546 14.224690303289192 6.334543642318098 68.01758507760216 10-11 14.51302862024776 34.26153353267834 30.692901893777584 20.532535953296314 12-13 14.550405809483127 8.528228677203474 35.60266267976648 41.318702833546915 14-15 15.051438131852485 7.491456642460487 12.128897906877404 65.32820731880963 16-17 39.77466894489534 29.847465470596614 6.76527125160188 23.612594332906163 18-19 40.75270539655418 13.543891499359248 6.428876548483554 39.27452655560302 20-21 16.761889505909156 8.523779011818311 6.710095400825858 68.00423608144668 22-23 15.769614125017798 32.855439270966826 6.4351060800227815 44.93984052399259 24-25 39.90281930798804 33.23900042716787 6.47159333618112 20.386586928662965 26-27 41.73608144667521 36.43119037448384 6.856934358536238 14.975793820304714 28-29 39.71860316104229 14.215790972518866 7.126584080877119 38.93902178556173 30-31 41.941655987469744 32.33660828705681 7.211127723195215 18.51060800227823 32-33 20.035063363235082 32.28232236935782 8.190944041008116 39.49167022639897 34-35 36.06542787982344 13.998647301722912 9.334508044995015 40.60141677345864 36-37 18.990281930798805 29.547558023636622 33.8263562580094 17.635803787555176 38-39 13.589278086287912 12.695785276947175 35.94795671365513 37.76697992310979 40-41 13.940801651715793 7.623166737861313 36.532642745265555 41.90338886515734 42-43 38.08735583084152 7.756656699416204 34.82486117043998 19.33112629930229 44-45 15.535561725758223 31.439555745407944 37.53737718923537 15.487505339598462 46-47 37.87466182543073 9.503595329631212 15.581838245763919 37.039904599174136 48-49 36.71240922682614 7.116794817029759 11.218496369073046 44.95229958707105 50-51 16.72184251744269 7.421151929374911 36.014701694432574 39.84230385874982 52-53 11.89573544069486 7.4905667093834545 59.97525986045849 20.638437989463192 54-55 12.29887512459063 7.169300868574684 40.95294033888652 39.578883667948176 56-57 36.34308699985761 7.251174711661683 17.140111063648014 39.26562722483269 58-59 15.044318667236222 9.484016801936495 55.75163747686174 19.72002705396554 60-61 36.00402249750819 33.6626085718354 16.139826285063364 14.193542645593052 62-63 13.177239071621813 64.45607290331768 8.005837960985334 14.360850064075182 64-65 10.975544639043145 67.50320375907731 7.335718353979781 14.185533247899759 66-67 11.603837391428165 67.61800512601452 6.909440410081162 13.86871707247615 68-69 11.470347429873273 67.40620105368076 7.096326356258009 14.027125160187953 70-71 11.82098106222412 62.49021073615264 8.620781717214864 17.06802648440837 72-73 13.758365370924105 49.707212017656275 12.760750391570555 23.773672219849068 74-75 14.850313256443116 43.562224120746116 15.184928093407377 26.402534529403386 76 14.413356115620104 44.315107503915705 14.796027338744128 26.475509041720063 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 5.0 19 12.5 20 11.0 21 7.0 22 12.0 23 14.5 24 14.0 25 16.0 26 29.0 27 36.0 28 46.5 29 63.0 30 76.0 31 71.0 32 78.0 33 103.0 34 118.0 35 138.5 36 158.0 37 172.0 38 174.0 39 180.0 40 390.0 41 4952.0 42 9308.0 43 9766.0 44 8890.0 45 5896.0 46 4236.0 47 3121.5 48 1486.5 49 710.0 50 454.0 51 373.5 52 328.5 53 352.0 54 340.0 55 388.0 56 479.0 57 592.0 58 662.0 59 709.5 60 846.0 61 952.0 62 969.0 63 1054.0 64 1176.0 65 1252.0 66 1229.0 67 1167.0 68 1143.0 69 1157.0 70 1194.0 71 1193.0 72 1136.5 73 1012.5 74 928.0 75 911.0 76 836.0 77 685.0 78 565.0 79 521.0 80 458.0 81 316.5 82 228.5 83 219.0 84 171.0 85 98.5 86 57.5 87 41.0 88 39.5 89 24.0 90 8.0 91 4.5 92 3.0 93 2.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 56184.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.500142389292325 #Duplication Level Percentage of deduplicated Percentage of total 1 58.630557688280874 14.9508756941478 2 24.359600753821457 12.423465755375195 3 10.539540727298109 8.06279367791542 4 3.824945906330704 3.90146660971095 5 1.3191875479863195 1.6819735155916276 6 0.44670901095833043 0.6834686031610423 7 0.20939484888671736 0.373771892353695 8 0.06281845466601522 0.12815036309269542 9 0.06979828296223912 0.1601879538658693 >10 0.3908703845885392 2.0753239356400397 >50 0.03489914148111956 0.663890075466325 >100 0.06979828296223912 4.36601167592197 >500 0.020939484888671738 3.9655417912572974 >1k 0.013959656592447826 7.466538516303574 >5k 0.0 0.0 >10k+ 0.006979828296223913 39.09653994019649 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 21966 39.09653994019649 No Hit TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 2642 4.702406379040296 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 1553 2.7641321372632777 No Hit ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT 938 1.669514452513171 TruSeq Adapter, Index 2 (95% over 21bp) TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 706 1.2565855047700412 No Hit CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC 584 1.0394418339740852 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT 461 0.8205182970240638 No Hit CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC 445 0.7920404385590203 No Hit CTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 236 0.4200484123593906 No Hit TTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC 229 0.4075893492809341 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 218 0.38801082158621675 No Hit TATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCT 206 0.3666524277374341 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGT 200 0.3559732308130429 No Hit CTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 183 0.3257155061939342 No Hit TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCG 170 0.30257724619108645 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAGCTCGTATGCCGT 105 0.1868859461768475 No Hit CCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 91 0.1619678200199345 No Hit TCTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 80 0.14238929232521716 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGCACGCATCTCGTATGCCGT 76 0.1352698277089563 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGCACGGATCTCGTATGCCGT 71 0.12637049693863023 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0017798661540652143 0.0 5 0.0 0.0 0.0 0.0035597323081304286 0.0 6 0.0 0.0 0.0 0.0035597323081304286 0.0 7 0.0 0.0 0.0 0.007119464616260857 0.0 8 0.0 0.0 0.0 0.00889933077032607 0.0 9 0.0 0.0 0.0 0.014238929232521715 0.0 10 0.0 0.0 0.0 0.01957852769471736 0.0 11 0.0 0.0 0.0 0.024918126156913 0.0 12 0.0 0.0 0.0 0.024918126156913 0.0 13 0.0 0.0 0.0 0.02847785846504343 0.0 14 0.0 0.0 0.0 0.02847785846504343 0.0 15 0.0 0.0 0.0 0.02847785846504343 0.0 16 0.0 0.0 0.0 0.02847785846504343 0.0 17 0.0 0.0 0.0 0.02847785846504343 0.0 18 0.0 0.0 0.0 0.02847785846504343 0.0 19 0.0 0.0 0.0 0.032037590773173856 0.0 20 0.0 0.0 0.0 0.04449665385163036 0.0 21 0.0 0.0 0.0 0.04805638615976079 0.0 22 0.0 0.0 0.0 0.05339598462195643 0.0 23 0.0 0.0 0.0 0.058735583084152075 0.0 24 0.0 0.0 0.0 0.060515449238217285 0.0 25 0.0 0.0 0.0 0.06407518154634771 0.0 26 0.0 0.0 0.0 0.07119464616260857 0.0 27 0.0 0.0 0.0 0.11569130001423893 0.0 28 0.0 0.0 0.0 0.17086715079026057 0.0 29 0.0 0.0 0.0 0.2349423323366083 0.0 30 0.0 0.0 0.0 0.3007973800370212 0.0 31 0.0 0.0 0.0 0.35775309696710805 0.0 32 0.0 0.0 0.0 0.4681047985191514 0.0 33 0.0 0.0 0.0 0.5891356969955859 0.0 34 0.0 0.0 0.0 0.8187384308699985 0.0 35 0.0 0.0 0.0 1.0821586216716503 0.0 36 0.0 0.0 0.0 1.4772889078741278 0.0 37 0.0 0.0 0.0 1.9987896910152356 0.0 38 0.0 0.0 0.0 2.623522711092126 0.0 39 0.0 0.0 0.0 3.1414637619251033 0.0 40 0.0 0.0 0.0 3.627367221984907 0.0 41 0.0 0.0 0.0 4.038516303573971 0.0 42 0.0 0.0 0.0 4.438986188238644 0.0 43 0.0 0.0 0.0 4.898191655987469 0.0 44 0.0 0.0 0.0 5.414352840666382 0.0 45 0.0 0.0 0.0 5.951872419194077 0.0 46 0.0 0.0 0.0 6.487612131567706 0.0 47 0.0 0.0 0.0 6.896981347002705 0.0 48 0.0 0.0 0.0 7.317029759362096 0.0 49 0.0 0.0 0.0 7.610707674782856 0.0 50 0.0 0.0 0.0 7.956001708671508 0.0 51 0.0 0.0 0.0 8.329773601025202 0.0 52 0.0 0.0 0.0 8.653709241065071 0.0 53 0.0 0.0 0.0 8.95984621956429 0.0 54 0.0 0.0 0.0 9.283781859604158 0.0 55 0.0 0.0 0.0 9.605937633489962 0.0 56 0.0 0.0 0.0 9.910294745835113 0.0 57 0.0 0.0 0.0 10.189733732023353 0.0 58 0.0 0.0 0.0 10.51010963975509 0.0 59 0.0 0.0 0.0 10.7859888936352 0.0 60 0.0 0.0 0.0 11.072547344439698 0.0 61 0.0 0.0 0.0 11.359105795244197 0.0 62 0.0 0.0 0.0 11.677701836821871 0.0 63 0.0 0.0 0.0 11.948241492239784 0.0 64 0.0 0.0 0.0 12.170724761497935 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACAACT 15 0.0022138702 70.0 70 AAATTTT 15 0.0022138702 70.0 69 AAATCCC 15 0.0022138702 70.0 70 CACGCAT 15 0.0022138702 70.0 32 ACACAAA 15 0.0022138702 70.0 70 CCTTATA 20 7.863229E-5 70.0 1 CGCATCT 15 0.0022138702 70.0 34 TCTTAAT 20 7.863229E-5 70.0 1 TCTTTAT 65 0.0 64.61539 1 CTTTACA 35 2.9249168E-7 60.000004 1 ACAACAC 25 2.3714367E-4 55.999996 70 AAATCCG 20 0.006917822 52.5 68 ACGCATC 20 0.006917822 52.5 33 GCACGCA 20 0.006917822 52.5 31 AATACAA 20 0.006917822 52.5 69 GCATCTC 20 0.006917822 52.5 35 TTTACAC 40 7.356466E-7 52.5 2 GAGACGT 20 0.006917822 52.5 24 ACGTGCA 20 0.006917822 52.5 27 TCTCGTA 3355 0.0 52.056633 38 >>END_MODULE