FastQCFastQC Report
Wed 25 May 2016
SRR1779776_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779776_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71565
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACT150.002216978269.9951169
CCTAGTG150.002216978269.9951132
AAGCGTA150.002216978269.9951134
CTAGGGG150.002216978269.9951144
ATGTATA308.303827E-658.32925428
AGTATGC308.303827E-658.32925428
AGTGTCG308.303827E-658.32925435
ATCTCGG1000.055.9960942
GTGGCAG252.3766825E-455.9960948
GAAAATT252.3766825E-455.9960926
TCGTTGT452.6573616E-854.44064345
CCGTATC653.6379788E-1253.8423955
AGCGTGT200.006927469352.49633433
GTACCAT200.006927469352.49633457
AATTTGC200.006927469352.49633429
GCAGTGT407.388826E-752.49633433
CATCTAG200.006927469352.49633441
TAACGCT200.006927469352.49633470
GTGGATC200.006927469352.49633438
TTCCCGA200.006927469352.4963342
Produced by FastQC (version 0.11.5)