##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779776_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	71565
Sequences flagged as poor quality	0
Sequence length	76
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.577642702438343	32.0	21.0	34.0	12.0	34.0
2	25.9625654998952	31.0	21.0	34.0	12.0	34.0
3	25.143338223992174	27.0	21.0	34.0	11.0	34.0
4	26.3585411863341	32.0	21.0	34.0	12.0	34.0
5	26.124013134912317	32.0	21.0	34.0	11.0	34.0
6	27.93679871445539	34.0	21.0	37.0	11.0	38.0
7	28.870048207922867	34.0	21.0	38.0	12.0	38.0
8	28.123174736253755	34.0	21.0	38.0	11.0	38.0
9	29.805030391951373	34.0	23.0	38.0	12.0	38.0
10-11	29.051554530846083	34.0	21.0	38.0	11.0	38.0
12-13	28.732508907985746	33.5	22.0	37.5	11.0	38.0
14-15	28.153238314818697	33.5	21.0	37.5	11.0	38.0
16-17	28.075309159505345	32.5	16.0	37.5	11.0	38.0
18-19	28.5402920421994	34.0	21.0	37.5	11.0	38.0
20-21	27.551470690980228	32.5	16.0	37.0	11.0	38.0
22-23	27.634800530985817	31.0	16.0	37.5	11.0	38.0
24-25	28.31145811500035	33.5	21.0	37.5	11.0	38.0
26-27	27.151233144693634	32.0	16.0	37.0	11.0	38.0
28-29	28.614441416893733	34.0	21.0	38.0	11.0	38.0
30-31	29.10412911339342	34.5	21.0	38.0	11.0	38.0
32-33	29.266121707538602	35.0	21.0	38.0	11.0	38.0
34-35	29.30846083979599	35.5	21.0	38.0	11.0	38.0
36-37	27.74983581359603	32.5	16.0	37.5	11.0	38.0
38-39	26.69717739118284	29.0	16.0	37.0	11.0	38.0
40-41	27.12020540767135	31.0	16.0	37.0	11.0	38.0
42-43	27.935925382519386	32.5	21.0	37.5	11.0	38.0
44-45	29.355089778523023	35.0	21.5	38.0	11.0	38.0
46-47	28.294787955005937	33.5	16.0	37.5	11.0	38.0
48-49	27.528701180744775	32.5	16.0	37.0	11.0	38.0
50-51	25.954460979529102	27.0	11.0	37.0	11.0	38.0
52-53	28.310088730524697	34.0	21.0	37.5	11.0	38.0
54-55	27.63828687207434	32.5	16.0	37.5	11.0	38.0
56-57	28.035024103961433	32.5	16.0	38.0	11.0	38.0
58-59	28.8593586250262	34.5	21.0	38.0	11.0	38.0
60-61	29.424551107384893	36.0	21.0	38.0	11.0	38.0
62-63	29.33354293299797	35.5	21.0	38.0	11.0	38.0
64-65	29.115706001537063	35.0	21.0	38.0	11.0	38.0
66-67	27.619981834695732	33.0	21.0	37.5	11.0	38.0
68-69	27.54542723398309	32.5	20.5	37.0	11.0	38.0
70-71	27.251631384056452	31.5	20.0	37.0	11.0	38.0
72-73	26.36859498358136	28.0	14.5	37.0	11.0	38.0
74-75	27.486138475511773	32.5	19.5	37.0	11.0	38.0
76	27.504422552923916	33.0	19.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	1.0
5	2.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	8.0
12	39.0
13	235.0
14	616.0
15	1186.0
16	2014.0
17	3086.0
18	4007.0
19	4373.0
20	3739.0
21	3050.0
22	2259.0
23	1722.0
24	1595.0
25	1357.0
26	1452.0
27	1536.0
28	1735.0
29	1953.0
30	2350.0
31	2810.0
32	3523.0
33	4307.0
34	5338.0
35	6781.0
36	6978.0
37	3512.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.959337665059735	16.44239502550129	12.271361699154614	37.326905610284356
2	18.18766156640816	13.598826241878012	44.28561447635017	23.927897715363656
3	18.313421365192482	14.69852581569203	40.64416963599525	26.34388318312024
4	15.110738489485085	19.450848878641793	26.19157409348145	39.24683853839167
5	15.759100118773143	23.05456577936142	39.18815063229232	21.998183469573114
6	27.5791238734018	32.26996436805701	13.952351009571718	26.19856074896947
7	25.71089219590582	20.42199399147628	18.57053028715154	35.29658352546636
8	21.563120091668765	30.966155222045224	17.57916212515022	29.8915625611358
9	20.57097342164836	14.032587127246304	17.2815181241441	48.11492132696124
10-11	24.658328675237563	20.495388485187256	26.16405813303522	28.682224706539966
12-13	22.952446164810443	15.19542767708668	30.19102583809617	31.661100320006707
14-15	25.687695199234224	19.186294306295984	21.549296758732	33.57671373573779
16-17	28.69799745664417	17.23892172892299	22.706438044131417	31.35664277030143
18-19	23.123790359206545	16.56290830835447	27.05472991384912	33.25857141858987
20-21	28.331074188454604	16.728385573147385	23.814647643269378	31.12589259512863
22-23	29.917551704863055	15.769284516489659	22.802543320290667	31.510620458356627
24-25	23.844358737877645	21.090103686313967	19.739526564377744	35.326011011430644
26-27	27.412784807473294	20.538976963870237	22.916652110422504	29.131586118233965
28-29	24.98917007867414	21.206383365240843	22.15382680510334	31.65061975098168
30-31	24.65955380412378	19.876887388992532	23.673674722787013	31.789884084096677
32-33	25.04855996981596	19.507832478584707	23.928536493341348	31.515071058257988
34-35	24.662176325093277	20.2023448526432	23.519095596763602	31.616383225499924
36-37	28.199216047959418	15.746815631528566	28.74071589773688	27.313252422775136
38-39	27.508384572386806	14.461989938513137	33.52920626048071	24.500419228619343
40-41	25.839674121896856	20.94311806094144	22.75557046135787	30.461637355803827
42-43	21.297616053212597	21.667924317375142	25.408736480254884	31.625723149157377
44-45	20.967572892866876	17.276989400577133	29.155050621502088	32.6003870850539
46-47	26.602991832200274	16.93787860791068	26.844043235538663	29.615086324350383
48-49	26.400737837214667	17.214804256538173	28.278868929087974	28.105588977159186
50-51	32.26989749931876	15.695809839226948	27.081979583708886	24.952313077745405
52-53	20.4552759184472	19.84251198278392	27.918139768868517	31.784072329900365
54-55	25.3294392196867	18.080378977375943	25.534159667975576	31.05602213496178
56-57	25.384980855761434	18.70825298342696	24.65274866549286	31.25401749531874
58-59	21.26327557294578	20.53311906092789	26.788010061486865	31.415595304639467
60-61	20.793739519284514	19.040665176076022	26.37227501397429	33.79332029066517
62-63	21.06329609211786	20.658743301122826	26.00806310744056	32.26989749931876
64-65	20.918517069353417	21.76464834197398	25.18480736714132	32.13202722153128
66-67	19.3921185019564	27.345584125209616	19.478759083286754	33.78353828954723
68-69	19.842617634931617	26.97374398110293	19.588234060842403	33.59540432312305
70-71	19.247484628283956	28.783538289547234	18.499161542761318	33.46981553940749
72-73	18.364053045653357	29.561492991992843	17.93784323863557	34.13661072371823
74-75	20.836768630958204	24.54339654280963	21.153281815514035	33.46655301071813
76	22.67715655175305	22.98179175808052	22.397674711085646	31.943376979080785
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	1.0
6	0.5
7	0.5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	2.0
17	3.0
18	4.5
19	5.5
20	9.0
21	13.0
22	16.5
23	26.5
24	52.5
25	72.0
26	95.5
27	138.0
28	199.0
29	241.0
30	287.0
31	398.0
32	534.5
33	606.0
34	716.0
35	926.5
36	1150.5
37	1274.0
38	1426.5
39	1675.0
40	1908.0
41	2178.5
42	2312.0
43	2382.0
44	2589.5
45	2722.0
46	2717.0
47	2730.0
48	2675.0
49	2550.0
50	2493.0
51	2432.0
52	2374.5
53	2283.0
54	2188.0
55	2167.5
56	2128.5
57	2153.0
58	2196.0
59	2203.0
60	2208.0
61	2271.0
62	2336.0
63	2335.0
64	2282.5
65	2243.0
66	2190.0
67	2125.0
68	2111.0
69	2036.0
70	1891.0
71	1807.0
72	1754.5
73	1649.5
74	1417.0
75	1237.0
76	1132.0
77	928.5
78	700.0
79	570.0
80	480.0
81	320.5
82	206.0
83	161.0
84	123.0
85	68.0
86	39.0
87	27.0
88	19.5
89	8.5
90	3.0
91	0.5
92	0.0
93	1.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.004191993292810731
9	0.004191993292810731
10-11	0.006986655488017886
12-13	0.005589324390414308
14-15	0.00489065884161252
16-17	0.008383986585621462
18-19	0.006287989939216097
20-21	0.005589324390414308
22-23	0.006986655488017886
24-25	0.004191993292810731
26-27	0.00489065884161252
28-29	0.005589324390414308
30-31	0.006287989939216097
32-33	0.005589324390414308
34-35	0.005589324390414308
36-37	0.006287989939216097
38-39	0.006986655488017886
40-41	0.006287989939216097
42-43	0.004191993292810731
44-45	0.006287989939216097
46-47	0.00489065884161252
48-49	0.006287989939216097
50-51	0.006287989939216097
52-53	0.005589324390414308
54-55	0.005589324390414308
56-57	0.004191993292810731
58-59	0.006986655488017886
60-61	0.006986655488017886
62-63	0.006287989939216097
64-65	0.005589324390414308
66-67	0.006986655488017886
68-69	0.027247956403269755
70-71	0.006986655488017886
72-73	0.005589324390414308
74-75	0.005589324390414308
76	0.005589324390414308
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	71565.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.5146370432474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.67805168647698	97.21232446028087
2	1.1602507730277154	2.286033675679452
3	0.14042154832487022	0.41500733598826245
4	0.018439193214376898	0.07266121707538602
5	0.0028367989560579843	0.013973310976035772
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0013973310976035773	0.0
4	0.0	0.0	0.0	0.0013973310976035773	0.0
5	0.0	0.0	0.0	0.004191993292810731	0.0
6	0.0	0.0	0.0	0.004191993292810731	0.0
7	0.0	0.0	0.0	0.004191993292810731	0.0
8	0.0	0.0	0.0	0.005589324390414309	0.0
9	0.0	0.0	0.0	0.008383986585621462	0.0
10	0.0	0.0	0.0	0.00978131768322504	0.0
11	0.0	0.0	0.0	0.00978131768322504	0.0
12	0.0	0.0	0.0	0.00978131768322504	0.0
13	0.0	0.0	0.0	0.00978131768322504	0.0
14	0.0	0.0	0.0	0.012575979878432194	0.0
15	0.0	0.0	0.0	0.012575979878432194	0.0
16	0.0	0.0	0.0	0.012575979878432194	0.0
17	0.0	0.0	0.0	0.013973310976035772	0.0
18	0.0	0.0	0.0	0.016767973171242925	0.0
19	0.0	0.0	0.0	0.022357297561657236	0.0
20	0.0	0.0	0.0	0.023754628659260812	0.0
21	0.0	0.0	0.0	0.032138615244882277	0.0
22	0.0	0.0	0.0	0.03772793963529658	0.0
23	0.0	0.0	0.0	0.04331726402571089	0.0
24	0.0	0.0	0.0	0.05729057500174666	0.0
25	0.0	0.0	0.0	0.06008523719695382	0.0
26	0.0	0.0	0.0	0.06986655488017886	0.0
27	0.0	0.0	0.0	0.11737581219870048	0.0
28	0.0	0.0	0.0	0.1774610493956543	0.0
29	0.0	0.0	0.0	0.19981834695731154	0.0
30	0.0	0.0	0.0	0.2515195975686439	0.0
31	0.0	0.0	0.0	0.27806888842311184	0.0
32	0.0	0.0	0.0	0.31998882135121914	0.0
33	0.0	0.0	0.0	0.36749807866974077	0.0
34	0.0	0.0	0.0	0.46810591769719834	0.0
35	0.0	0.0	0.0	0.6469642981904562	0.0
36	0.0	0.0	0.0	0.7559561238035353	0.0
37	0.0	0.0	0.0	1.166771466498987	0.0
38	0.0	0.0	0.0	1.496541605533431	0.0
39	0.0	0.0	0.0	1.8626423531055685	0.0
40	0.0	0.0	0.0	2.2594843848249844	0.0
41	0.0	0.0	0.0	2.680081045203661	0.0
42	0.0	0.0	0.0	3.069936421435059	0.0
43	0.0	0.0	0.0	3.4681757842520784	0.0
44	0.0	0.0	0.0	3.929295046461259	0.0
45	0.0	0.0	0.0	4.243694543422063	0.0
46	0.0	0.0	0.0	4.588835324530147	0.0
47	0.0	0.0	0.0	5.0317892824704815	0.0013973310976035773
48	0.0	0.0	0.0	5.283308880039125	0.0013973310976035773
49	0.0	0.0	0.0	5.541815133095787	0.0013973310976035773
50	0.0	0.0	0.0	5.88695591420387	0.0013973310976035773
51	0.0	0.0	0.0	6.3396911898274295	0.0013973310976035773
52	0.0	0.0	0.0	6.672255991057081	0.0013973310976035773
53	0.0	0.0	0.0	7.070495353874101	0.0013973310976035773
54	0.0	0.0	0.0	7.442185425836652	0.0013973310976035773
55	0.0	0.0	0.0	7.709075665478935	0.0013973310976035773
56	0.0	0.0	0.0	8.059805770977434	0.0013973310976035773
57	0.0	0.0	0.0	8.277789422203591	0.0013973310976035773
58	0.0	0.0	0.0	8.511143715503389	0.0013973310976035773
59	0.0	0.0	0.0	8.757073988681618	0.0013973310976035773
60	0.0	0.0	0.0	8.907985747222805	0.0013973310976035773
61	0.0	0.0	0.0	9.049116188080765	0.0013973310976035773
62	0.0	0.0	0.0	9.181862642353106	0.0013973310976035773
63	0.0	0.0	0.0	9.352337036260742	0.0013973310976035773
64	0.0	0.0	0.0	9.525606092363585	0.0013973310976035773
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCACT	15	0.0022169782	69.99511	69
CCTAGTG	15	0.0022169782	69.99511	32
AAGCGTA	15	0.0022169782	69.99511	34
CTAGGGG	15	0.0022169782	69.99511	44
ATGTATA	30	8.303827E-6	58.329254	28
AGTATGC	30	8.303827E-6	58.329254	28
AGTGTCG	30	8.303827E-6	58.329254	35
ATCTCGG	100	0.0	55.99609	42
GTGGCAG	25	2.3766825E-4	55.99609	48
GAAAATT	25	2.3766825E-4	55.99609	26
TCGTTGT	45	2.6573616E-8	54.440643	45
CCGTATC	65	3.6379788E-12	53.84239	55
AGCGTGT	20	0.0069274693	52.496334	33
GTACCAT	20	0.0069274693	52.496334	57
AATTTGC	20	0.0069274693	52.496334	29
GCAGTGT	40	7.388826E-7	52.496334	33
CATCTAG	20	0.0069274693	52.496334	41
TAACGCT	20	0.0069274693	52.496334	70
GTGGATC	20	0.0069274693	52.496334	38
TTCCCGA	20	0.0069274693	52.496334	2
>>END_MODULE