FastQCFastQC Report
Wed 25 May 2016
SRR1779776_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779776_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71565
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT1677023.43324250681199No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG19882.7778942220359113No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC11201.5650108293160065No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT8071.1276461957660866RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC6350.8873052469782715No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC5410.7559561238035352No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT3720.5198071683085307No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC3380.4722979109900091No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG2170.30322084817997624No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC1830.2557115908614546No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG1800.2515195975686439No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC1800.2515195975686439No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT1460.20401034025012227No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT1350.1886396981764829No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCG1230.17187172500523998No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAGCTCGTATGCCGT1180.16488506951722212No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT740.1034025012226647No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGC150.002216363870.000011
ACAATAA150.002216363870.0000170
TCCAACG150.002216363870.000012
TGGATGT150.002216363870.000015
TCTTTAT750.065.3333361
CTATACA1050.063.3333321
TTTACAC401.0482836E-861.2500042
TCTTAAT308.300387E-658.3333361
CTTTACA452.6559064E-854.4444471
TCCTGTA200.006925555452.50000415
AGCTTAT200.006925555452.5000042
TAGAACT200.006925555452.50000434
AAGAATA200.006925555452.50000470
TCAGGAG200.006925555452.50000438
CAGGGAT200.006925555452.5000044
GTTGTAA200.006925555452.5000041
GCTTATA200.006925555452.5000043
TTGGTCA200.006925555452.50000444
TCACTGT200.006925555452.5000046
CAGCTTA200.006925555452.5000041
Produced by FastQC (version 0.11.5)