FastQCFastQC Report
Wed 25 May 2016
SRR1779775_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779775_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49016
Sequences flagged as poor quality0
Sequence length76
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTG252.8005834E-670.0000145
GTGTACC252.8005834E-670.0000136
CGTGTAA252.8005834E-670.0000135
TGTAGAC252.8005834E-670.0000137
TGTACCT252.8005834E-670.0000137
CGTTTAG252.8005834E-670.0000135
TTGTGTC150.0022121870.034
GTTTAGA353.616151E-970.036
TGAGTGT150.0022121870.033
GCGTTTA150.0022121870.034
CATTCAA150.0022121870.061
GTGGATC150.0022121870.054
GCCGATA150.0022121870.020
TAACCTT150.0022121870.058
TCGTGTA550.070.034
ACGTGTA207.85323E-570.034
AATTATT301.0039548E-770.057
CGTGTAT207.85323E-570.035
TGAGGCT150.0022121870.013
CACGTGT150.0022121870.033
Produced by FastQC (version 0.11.5)