##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779775_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49016
Sequences flagged as poor quality	0
Sequence length	76
%GC	52
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.42569773135303	23.0	12.0	32.0	12.0	34.0
2	23.23102660355802	23.0	12.0	32.0	12.0	34.0
3	23.083584951852455	23.0	11.0	32.0	11.0	34.0
4	22.308123877917414	23.0	12.0	32.0	11.0	34.0
5	22.973580055492086	23.0	11.0	32.0	11.0	34.0
6	23.50632446548066	26.0	11.0	34.0	11.0	38.0
7	24.355700179533212	26.0	12.0	34.0	12.0	38.0
8	23.781153092867633	26.0	11.0	34.0	11.0	38.0
9	25.24492002611392	27.0	12.0	36.0	12.0	38.0
10-11	24.144677248245472	26.0	11.0	35.0	11.0	38.0
12-13	24.760700587563242	26.5	11.0	35.0	11.0	38.0
14-15	23.399900032642403	25.5	11.0	34.0	11.0	38.0
16-17	23.57256814101518	24.5	11.0	34.5	11.0	38.0
18-19	24.020554512812144	26.0	11.5	34.0	11.0	38.0
20-21	23.247133589032153	23.5	11.0	34.0	11.0	37.5
22-23	23.491880202382895	24.5	11.0	33.0	11.0	37.5
24-25	23.302360453729392	23.0	11.0	34.0	11.0	37.5
26-27	22.53327484902889	23.0	11.0	33.0	10.0	37.5
28-29	23.0505447200914	23.0	11.0	34.0	11.0	38.0
30-31	23.50500856863065	24.0	11.0	34.5	11.0	38.0
32-33	23.69316141668027	25.0	11.0	35.0	11.0	38.0
34-35	23.65131793700016	24.5	11.0	35.0	11.0	38.0
36-37	23.430084054186388	24.0	11.0	34.0	11.0	38.0
38-39	23.58716541537457	24.5	11.0	34.0	11.0	37.0
40-41	22.601976905500244	23.0	11.0	34.0	11.0	37.0
42-43	23.004998367879878	23.5	11.0	34.0	11.0	37.5
44-45	23.771911212665252	26.0	11.0	34.5	11.0	38.0
46-47	23.471315488819975	24.5	11.0	35.0	11.0	38.0
48-49	22.74625632446548	23.0	11.0	34.0	11.0	37.5
50-51	22.231628447853762	23.0	11.0	31.5	11.0	37.0
52-53	22.626152684837606	23.0	11.0	34.0	11.0	37.5
54-55	22.627825607964745	23.0	11.0	33.5	11.0	37.5
56-57	22.901899379794354	23.0	11.0	34.0	11.0	38.0
58-59	22.95726905500245	23.0	11.0	34.0	11.0	38.0
60-61	23.588420107719926	24.0	11.0	35.5	11.0	38.0
62-63	23.57764811490126	24.0	11.0	35.5	11.0	38.0
64-65	23.61697200913987	24.5	11.0	35.0	11.0	38.0
66-67	23.173637179696428	24.0	11.0	34.0	11.0	38.0
68-69	22.830963358903215	23.5	11.0	34.0	11.0	37.5
70-71	22.831177574669496	23.5	11.0	34.0	11.0	37.5
72-73	22.620878896686797	23.5	11.0	33.0	11.0	37.0
74-75	22.278959931450956	22.5	11.0	33.5	11.0	37.5
76	21.766076383221804	21.0	11.0	33.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
3	3.0
4	0.0
5	1.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	8.0
12	80.0
13	375.0
14	881.0
15	1596.0
16	2671.0
17	4128.0
18	5490.0
19	5719.0
20	4945.0
21	3578.0
22	2463.0
23	1613.0
24	1198.0
25	928.0
26	744.0
27	670.0
28	683.0
29	678.0
30	804.0
31	875.0
32	1024.0
33	1278.0
34	1609.0
35	2018.0
36	1984.0
37	972.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.747347804798434	15.40517382079321	14.691121266525217	46.15635710788314
2	12.169495674881672	11.877754202709319	53.48457646482782	22.468173657581197
3	14.31777378815081	10.898482128284643	52.37269463032479	22.41104945323976
4	12.58772645666721	24.04724987759099	27.435939285131383	35.92908438061041
5	12.018524563407867	18.416435449649093	49.259425493716336	20.305614493226702
6	18.636771666394647	36.626815733638	16.525216255916437	28.211196344050922
7	17.963522115227683	20.701403623306675	18.865268483760406	42.46980577770524
8	14.800457030931199	34.14673957398188	16.76528197176202	34.287521423324904
9	15.457438994531952	15.310536195217498	17.64669876764874	51.58532604260181
10-11	17.370235860605565	21.801803639924916	30.576185423977805	30.251775075491715
12-13	15.658889421654543	14.370390018465432	36.66357209169463	33.30714846818539
14-15	23.706975700324403	21.364739966947543	23.21220900577398	31.716075326954073
16-17	29.520923874232313	15.281263389851258	22.064434514700782	33.13337822121565
18-19	16.873418754590713	14.580102831959522	32.50122418999429	36.04525422345548
20-21	28.988819064718847	14.365869582959276	23.799273647270056	32.84603770505182
22-23	31.997265975679422	12.832571615114666	21.477393291438833	33.69276911776708
24-25	18.360809597649556	22.961723659511957	20.1756712641802	38.50179547865829
26-27	27.796706996103076	22.101279252443227	22.269601942341826	27.832411809111868
28-29	20.37970292989472	21.486574716395985	24.832694034114095	33.3010283195952
30-31	19.104894819530312	20.381138927995757	27.368345881536797	33.14562037093714
32-33	20.732677711580838	18.816820370521505	27.39533175548845	33.055170162409205
34-35	19.875947481713474	20.604347959152445	26.423390428776923	33.096314130357165
36-37	28.09034614325209	13.59374840598635	32.50971710720953	25.806188343552023
38-39	28.50584562649201	10.803697129215891	38.73212136051091	21.958335883781192
40-41	22.92978035318962	23.529651809306166	24.39069179053468	29.149876046969524
42-43	14.800306044376436	24.156082631981636	29.63631726600357	31.407294057638357
44-45	15.295389857482428	15.366801668996052	35.010150678922294	34.327657794599226
46-47	27.338202889088386	14.935117930302782	27.107647106830978	30.619032073777852
48-49	25.93983044795609	15.12400150984973	32.246513573345034	26.68965446884915
50-51	41.12114256567202	12.68859984697781	24.108135679673552	22.082121907676612
52-53	14.682268447201166	19.208757128429042	32.640298705405876	33.468675718963915
54-55	25.69015119054906	15.754626512415582	26.064556936197995	32.49066536083736
56-57	25.769693754718137	16.821047477199926	24.49656213657601	32.91269663150593
58-59	15.500443773400121	20.54109749752609	30.8703059486039	33.08815278046989
60-61	15.273966314028339	17.525478714179325	30.491823347581693	36.70873162421064
62-63	15.48443700839616	20.79962457024515	29.80993868660797	33.90599973475072
64-65	15.546192769117766	22.45572512853995	28.48894148371827	33.50914061862401
66-67	13.007549479698021	34.968373801265045	18.677820852887166	33.34625586614977
68-69	14.22201361182821	33.22959501239758	18.9116659694091	33.63672540636511
70-71	12.91342759788619	36.04803003407399	17.752137275305543	33.286405092734284
72-73	12.005223210642292	38.97718925977311	16.637762180690444	32.37982534889415
74-75	15.351342528360401	26.964824940830816	21.48147392475312	36.20235860605566
76	17.636497184363012	24.2144780869991	24.179792703827633	33.96923202481025
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.5
6	1.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	0.0
15	0.5
16	1.5
17	2.0
18	5.5
19	10.0
20	10.5
21	10.0
22	13.0
23	20.5
24	40.0
25	55.0
26	65.5
27	101.5
28	146.0
29	165.0
30	213.0
31	307.0
32	399.0
33	445.0
34	524.5
35	696.5
36	892.0
37	995.0
38	1071.5
39	1266.5
40	1555.5
41	1759.5
42	1793.0
43	1937.5
44	2123.5
45	2222.0
46	2279.0
47	2301.0
48	2352.0
49	2331.5
50	2282.0
51	2235.5
52	2129.0
53	1940.0
54	1811.0
55	1796.5
56	1717.5
57	1628.5
58	1604.0
59	1545.0
60	1467.0
61	1375.5
62	1303.0
63	1322.0
64	1256.5
65	1129.0
66	1055.5
67	1025.0
68	959.5
69	886.0
70	788.0
71	698.0
72	696.5
73	648.0
74	532.5
75	464.0
76	429.0
77	352.0
78	273.0
79	236.0
80	194.5
81	120.5
82	71.5
83	55.0
84	43.5
85	27.0
86	14.5
87	7.0
88	5.5
89	2.0
90	1.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.008160600620205648
9	0.008160600620205648
10-11	0.008160600620205648
12-13	0.011220825852782765
14-15	0.006120450465154235
16-17	0.01020075077525706
18-19	0.008160600620205648
20-21	0.008160600620205648
22-23	0.008160600620205648
24-25	0.008160600620205648
26-27	0.006120450465154235
28-29	0.008160600620205648
30-31	0.01020075077525706
32-33	0.008160600620205648
34-35	0.009180675697731353
36-37	0.009180675697731353
38-39	0.01020075077525706
40-41	0.011220825852782765
42-43	0.007140525542679941
44-45	0.009180675697731353
46-47	0.008160600620205648
48-49	0.009180675697731353
50-51	0.007140525542679941
52-53	0.009180675697731353
54-55	0.01020075077525706
56-57	0.006120450465154235
58-59	0.009180675697731353
60-61	0.009180675697731353
62-63	0.011220825852782765
64-65	0.008160600620205648
66-67	0.01224090093030847
68-69	0.029582177248245474
70-71	0.01020075077525706
72-73	0.008160600620205648
74-75	0.008160600620205648
76	0.008160600620205648
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	49016.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.46344050922148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.52618300412283	98.9921658234046
2	0.42048694439316553	0.8364615635710788
3	0.04307427235247061	0.12852945976823896
4	0.008204623305232498	0.03264240248082259
5	0.0020511558263081246	0.01020075077525706
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.008160600620205648	0.0
6	0.0	0.0	0.0	0.008160600620205648	0.0
7	0.0	0.0	0.0	0.01020075077525706	0.0
8	0.0	0.0	0.0	0.01020075077525706	0.0
9	0.0	0.0	0.0	0.01020075077525706	0.0
10	0.0	0.0	0.0	0.01020075077525706	0.0
11	0.0	0.0	0.0	0.01020075077525706	0.0
12	0.0	0.0	0.0	0.01020075077525706	0.0
13	0.0	0.0	0.0	0.01224090093030847	0.0
14	0.0	0.0	0.0	0.02040150155051412	0.0
15	0.0	0.0	0.0	0.02040150155051412	0.0
16	0.0	0.0	0.0	0.02040150155051412	0.0
17	0.0	0.0	0.0	0.02040150155051412	0.0
18	0.0	0.0	0.0	0.02040150155051412	0.0
19	0.0	0.0	0.0	0.03672270279092541	0.0
20	0.0	0.0	0.0	0.04080300310102824	0.0
21	0.0	0.0	0.0	0.04488330341113106	0.0
22	0.0	0.0	0.0	0.07140525542679942	0.0
23	0.0	0.0	0.0	0.07752570589195365	0.0
24	0.0	0.0	0.0	0.10404765790762201	0.0
25	0.0	0.0	0.0	0.10404765790762201	0.0
26	0.0	0.0	0.0	0.10608780806267341	0.0
27	0.0	0.0	0.0	0.14077036069854743	0.0
28	0.0	0.0	0.0	0.17137261302431858	0.0
29	0.0	0.0	0.0	0.19585441488493552	0.0
30	0.0	0.0	0.0	0.23257711767586095	0.0
31	0.0	0.0	0.0	0.2631793700016321	0.0
32	0.0	0.0	0.0	0.3243838746531745	0.0
33	0.0	0.0	0.0	0.38558837930471684	0.0
34	0.0	0.0	0.0	0.44067243349110496	0.0
35	0.0	0.0	0.0	0.5671617431042925	0.0
36	0.0	0.0	0.0	0.6650889505467602	0.0
37	0.0	0.0	0.0	1.0241553778358088	0.0
38	0.0	0.0	0.0	1.2669332462869267	0.0
39	0.0	0.0	0.0	1.5423535172188674	0.0
40	0.0	0.0	0.0	1.8320548392361677	0.0
41	0.0	0.0	0.0	2.111555410478211	0.0
42	0.0	0.0	0.0	2.382895381100049	0.0
43	0.0	0.0	0.0	2.6379141504814756	0.0
44	0.0	0.0	0.0	2.93373592296393	0.0
45	0.0	0.0	0.0	3.082666884282683	0.0
46	0.0	0.0	0.0	3.298922800718133	0.0
47	0.0	0.0	0.0	3.5559817202546107	0.0
48	0.0	0.0	0.0	3.6783907295576954	0.0
49	0.0	0.0	0.0	3.835482291496654	0.0
50	0.0	0.0	0.0	4.059898808552309	0.0
51	0.0	0.0	0.0	4.296556226538273	0.0
52	0.0	0.0	0.0	4.514852293128774	0.0
53	0.0	0.0	0.0	4.833115717316795	0.0
54	0.0	0.0	0.0	5.041211033132038	0.0
55	0.0	0.0	0.0	5.226864697241717	0.0
56	0.0	0.0	0.0	5.373755508405418	0.0
57	0.0	0.0	0.0	5.532887220499429	0.0
58	0.0	0.0	0.0	5.663456830422719	0.0
59	0.0	0.0	0.0	5.845030194222295	0.0
60	0.0	0.0	0.0	5.926636200424351	0.0
61	0.0	0.0	0.0	6.02660355802187	0.0
62	0.0	0.0	0.0	6.098008813448669	0.0
63	0.0	0.0	0.0	6.224498123061857	0.0
64	0.0	0.0	0.0	6.306104129263914	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTG	25	2.8005834E-6	70.00001	45
GTGTACC	25	2.8005834E-6	70.00001	36
CGTGTAA	25	2.8005834E-6	70.00001	35
TGTAGAC	25	2.8005834E-6	70.00001	37
TGTACCT	25	2.8005834E-6	70.00001	37
CGTTTAG	25	2.8005834E-6	70.00001	35
TTGTGTC	15	0.00221218	70.0	34
GTTTAGA	35	3.616151E-9	70.0	36
TGAGTGT	15	0.00221218	70.0	33
GCGTTTA	15	0.00221218	70.0	34
CATTCAA	15	0.00221218	70.0	61
GTGGATC	15	0.00221218	70.0	54
GCCGATA	15	0.00221218	70.0	20
TAACCTT	15	0.00221218	70.0	58
TCGTGTA	55	0.0	70.0	34
ACGTGTA	20	7.85323E-5	70.0	34
AATTATT	30	1.0039548E-7	70.0	57
CGTGTAT	20	7.85323E-5	70.0	35
TGAGGCT	15	0.00221218	70.0	13
CACGTGT	15	0.00221218	70.0	33
>>END_MODULE