FastQCFastQC Report
Wed 25 May 2016
SRR1779775_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779775_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49016
Sequences flagged as poor quality0
Sequence length76
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2418549.341031499918394No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG28955.9062346988738375No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC14382.93373592296393No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT8351.7035253794679288Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC7301.4893096131875305No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC5871.1975681410151788No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT5041.0282356781459117No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC3750.7650563081442794No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG2380.485555736902236No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC2370.4835155867471846No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC1990.40598988085523097No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG1920.39170882976987104No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT1890.38558837930471684Illumina PCR Primer Index 9 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1710.3488656765137914No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT1630.33254447527338016No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT1590.3243838746531745No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT1030.21013546597029542No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG910.18565366410967848No Hit
TCTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT700.1428105108535988No Hit
CTCTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG550.11220825852782765No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCAT150.0022121870.069
AAACTAT150.0022121870.068
AAACTAC207.85323E-570.068
ACAGATT207.85323E-570.033
TTCGTAT207.85323E-570.039
AAACAGT150.0022121870.069
ATGGCGG150.0022121870.05
AACTACA150.0022121870.069
CAGATTT150.0022121870.034
GATTTCG150.0022121870.036
AAACGTT150.0022121870.069
AGATTTC150.0022121870.035
TCTTTAT550.070.01
ATTTCGT150.0022121870.037
TTTCGTA150.0022121870.038
TCTTAAT401.3096724E-1070.01
ACTACAA150.0022121870.070
CTTTACA501.2732926E-1163.0000041
AAATATA453.7289283E-1062.22221868
CTATACA601.8189894E-1258.3333321
Produced by FastQC (version 0.11.5)