##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779775_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49016
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.67394320221968	34.0	34.0	34.0	34.0	34.0
2	33.6864289211686	34.0	34.0	34.0	34.0	34.0
3	33.66984250040803	34.0	34.0	34.0	34.0	34.0
4	33.70295413742451	34.0	34.0	34.0	34.0	34.0
5	33.74357352701159	34.0	34.0	34.0	34.0	34.0
6	37.46072710951526	38.0	38.0	38.0	37.0	38.0
7	37.44440590827485	38.0	38.0	38.0	37.0	38.0
8	37.33174881671291	38.0	38.0	38.0	37.0	38.0
9	37.37628529459768	38.0	38.0	38.0	37.0	38.0
10-11	37.24816386486045	38.0	38.0	38.0	36.0	38.0
12-13	37.275644687449	38.0	38.0	38.0	36.0	38.0
14-15	37.27316590501061	38.0	38.0	38.0	36.0	38.0
16-17	37.057430226864696	38.0	38.0	38.0	35.5	38.0
18-19	37.00847682389424	38.0	38.0	38.0	35.0	38.0
20-21	37.14703362167455	38.0	38.0	38.0	36.0	38.0
22-23	37.158305451281215	38.0	38.0	38.0	36.0	38.0
24-25	36.95912559164354	38.0	38.0	38.0	34.5	38.0
26-27	36.75447812959034	38.0	37.5	38.0	34.5	38.0
28-29	36.807011996082906	38.0	37.5	38.0	34.5	38.0
30-31	36.83553329525053	38.0	37.0	38.0	35.0	38.0
32-33	36.56262240900931	38.0	37.0	38.0	34.0	38.0
34-35	36.39248408682879	38.0	37.0	38.0	34.0	38.0
36-37	36.336992002611396	38.0	37.0	38.0	34.0	38.0
38-39	33.48098580055492	37.0	34.0	38.0	23.0	38.0
40-41	35.862687693814266	37.5	36.5	38.0	32.5	38.0
42-43	36.80638975028562	38.0	37.0	38.0	34.5	38.0
44-45	36.869634405092214	38.0	37.5	38.0	34.5	38.0
46-47	36.85592459605027	38.0	37.0	38.0	35.0	38.0
48-49	36.79586257548556	38.0	37.0	38.0	34.5	38.0
50-51	36.570578994614	38.0	37.0	38.0	34.0	38.0
52-53	36.582626081279585	38.0	37.0	38.0	34.0	38.0
54-55	36.59228619226376	38.0	37.0	38.0	34.0	38.0
56-57	36.57488371144116	38.0	37.0	38.0	34.0	38.0
58-59	36.36117798269953	38.0	37.0	38.0	34.0	38.0
60-61	36.34122531418312	38.0	37.0	38.0	34.0	38.0
62-63	36.478568222621185	38.0	37.0	38.0	34.0	38.0
64-65	36.57380243185898	38.0	37.0	38.0	34.0	38.0
66-67	36.33407458788967	38.0	37.0	38.0	34.0	38.0
68-69	35.74455279908601	38.0	36.5	38.0	31.0	38.0
70-71	31.16877142157663	34.5	28.5	38.0	17.5	38.0
72-73	23.5604394483434	23.0	11.0	36.0	10.0	38.0
74-75	22.689040313367062	23.0	11.0	35.5	10.5	38.0
76	22.685939285131386	23.0	11.0	34.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	1.0
22	7.0
23	18.0
24	29.0
25	41.0
26	83.0
27	176.0
28	310.0
29	484.0
30	796.0
31	1241.0
32	1929.0
33	2940.0
34	5433.0
35	14753.0
36	12210.0
37	8564.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.289538110004898	5.685898482128285	12.799902072792557	68.22466133507426
2	7.244573200587563	4.800473314835972	69.69152929655623	18.263424188020238
3	7.293536804308798	8.10551656601926	70.88705728741635	13.71388934225559
4	6.550922147870083	57.670964582993314	21.739840052227844	14.038273216908765
5	6.716174310429247	20.103639627876614	65.54594418149176	7.634241880202383
6	11.940998857515913	65.31948751428105	11.757385343561285	10.98212828464175
7	10.496572547739513	18.716337522441652	9.40509221478701	61.38199771503182
8	8.91545617757467	66.50073445405582	6.1388118165496985	18.444997551819814
9	7.758691039660519	14.462624449159458	4.545454545454546	73.23322996572547
10-11	10.061000489636038	38.406846743920354	33.7063407866819	17.82581197976171
12-13	10.081401991186551	6.951811653337686	39.74824547086666	43.218540884609105
14-15	11.215725477395136	5.369675208095316	11.208584951852457	72.20601436265709
16-17	41.767994124367554	33.062673412763175	4.266974049290028	20.90235841357924
18-19	43.04104782111963	12.278643708176922	4.348580055492084	40.33172841521136
20-21	12.852945976823893	6.441774114574833	4.437326587236821	76.26795332136446
22-23	11.785947445732006	36.73494369185572	4.45874816386486	47.020360698547414
24-25	41.98935041619063	36.55133017790109	4.3026766770034275	17.15664272890485
26-27	43.98767749306349	39.687040966215115	5.470662640770361	10.854618899951037
28-29	40.12669332462869	12.68973396441978	7.364942059735596	39.81863065121593
30-31	16.103925248898317	7.466949567488166	63.24363473151624	13.185490452097273
32-33	9.872286600293782	64.75742614656438	14.5952342092378	10.775053043904032
34-35	10.788314019911866	43.693895870736085	5.849110494532398	39.66867961481965
36-37	39.717643218540886	39.54219030520647	7.220091398726947	13.520075077525707
38-39	12.602007507752571	11.848172025461073	36.49930634894728	39.05051411783907
40-41	10.146686796148197	5.473722866002937	40.216459931450956	44.16313040639791
42-43	40.01754529133344	5.678757956585605	37.931491757793374	16.37220499428758
44-45	12.145013873021055	35.39966541537457	41.38648604537294	11.068834666231435
46-47	39.71152276807573	7.395544312061368	14.265749959196997	38.627182960665905
48-49	38.47927207442468	4.719887383711441	8.717561612534682	48.0832789293292
50-51	13.9444263097764	5.192182144605844	39.706422392688104	41.156969152929655
52-53	8.174881671291006	5.099355312551004	68.92239268810184	17.803370328056147
54-55	8.87975354986127	4.884119471193079	44.827199281867145	41.408927697078504
56-57	38.570058756324464	4.952464501387302	15.708136118818345	40.76934062346989
58-59	11.765545944181492	7.222131548881998	64.48608617594255	16.526236330993964
60-61	38.357883140199114	36.78084707034437	14.93287905989881	9.928390729557696
62-63	10.053859964093357	74.7143789782928	5.402317610576138	9.829443447037702
64-65	7.329239432022197	77.87865186877754	4.932062999836788	9.860045699363473
66-67	7.260894401827975	77.92251509711114	4.752529786192264	10.064060714868614
68-69	7.3822833360535345	77.68177737881507	5.010608780806267	9.925330504325117
70-71	8.198343398074098	71.53786518687775	7.1201240411294275	13.14366737391872
72-73	10.268075730373756	55.428839562591804	12.87334747837441	21.429737228660027
74-75	11.396278766117186	48.79223110820956	15.484739676840215	24.326750448833035
76	10.702627713399707	50.08976660682226	14.90125673249551	24.30634894728252
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.5
17	3.0
18	3.5
19	5.5
20	5.5
21	4.0
22	5.5
23	7.5
24	10.5
25	13.0
26	14.0
27	19.0
28	24.0
29	25.0
30	26.0
31	44.5
32	54.0
33	46.0
34	50.5
35	59.0
36	86.5
37	110.0
38	274.5
39	5099.5
40	10300.5
41	9421.0
42	8001.0
43	6234.0
44	3243.0
45	1427.5
46	836.0
47	616.5
48	301.5
49	178.0
50	150.0
51	151.0
52	162.5
53	188.5
54	204.0
55	219.5
56	275.5
57	357.5
58	399.0
59	449.0
60	526.5
61	584.0
62	614.0
63	645.5
64	678.0
65	662.0
66	625.5
67	606.0
68	647.0
69	681.0
70	679.5
71	685.0
72	694.0
73	627.0
74	545.0
75	539.0
76	473.0
77	414.0
78	355.0
79	289.0
80	277.5
81	237.5
82	152.0
83	95.0
84	80.0
85	49.0
86	32.0
87	31.0
88	19.0
89	9.5
90	6.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	49016.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.76938142647299
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.20652173913044	11.675779337359229
2	23.347826086956523	8.764485066100864
3	9.01086956521739	5.073853435612861
4	3.0217391304347823	2.26864697241717
5	0.826086956521739	0.7752570589195364
6	0.43478260869565216	0.48963603721233884
7	0.13043478260869568	0.17137261302431858
8	0.08695652173913043	0.13056960992329036
9	0.06521739130434784	0.11016810837277623
>10	0.6521739130434783	2.366574179859638
>50	0.03260869565217392	0.4406724334911049
>100	0.10869565217391304	4.13334421413416
>500	0.043478260869565216	5.41863881181655
>1k	0.021739130434782608	8.839970621837768
>5k	0.0	0.0
>10k+	0.010869565217391304	49.341031499918394
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT	24185	49.341031499918394	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	2895	5.9062346988738375	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC	1438	2.93373592296393	No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT	835	1.7035253794679288	Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC	730	1.4893096131875305	No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC	587	1.1975681410151788	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT	504	1.0282356781459117	No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC	375	0.7650563081442794	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	238	0.485555736902236	No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC	237	0.4835155867471846	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC	199	0.40598988085523097	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG	192	0.39170882976987104	No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT	189	0.38558837930471684	Illumina PCR Primer Index 9 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	171	0.3488656765137914	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT	163	0.33254447527338016	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT	159	0.3243838746531745	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT	103	0.21013546597029542	No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	91	0.18565366410967848	No Hit
TCTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT	70	0.1428105108535988	No Hit
CTCTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG	55	0.11220825852782765	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.002040150155051412	0.0
3	0.0	0.0	0.0	0.002040150155051412	0.0
4	0.0	0.0	0.0	0.002040150155051412	0.0
5	0.0	0.0	0.0	0.01224090093030847	0.0
6	0.0	0.0	0.0	0.01224090093030847	0.0
7	0.0	0.0	0.0	0.014281051085359882	0.0
8	0.0	0.0	0.0	0.014281051085359882	0.0
9	0.0	0.0	0.0	0.014281051085359882	0.0
10	0.0	0.0	0.0	0.014281051085359882	0.0
11	0.0	0.0	0.0	0.014281051085359882	0.0
12	0.0	0.0	0.0	0.014281051085359882	0.0
13	0.0	0.0	0.0	0.02244165170556553	0.0
14	0.0	0.0	0.0	0.026521952015668353	0.0
15	0.0	0.0	0.0	0.026521952015668353	0.0
16	0.0	0.0	0.0	0.026521952015668353	0.0
17	0.0	0.0	0.0	0.026521952015668353	0.0
18	0.0	0.0	0.0	0.026521952015668353	0.0
19	0.0	0.0	0.0	0.042843153256079645	0.0
20	0.0	0.0	0.0	0.04896360372123388	0.0
21	0.0	0.0	0.0	0.05712420434143953	0.0
22	0.0	0.0	0.0	0.061204504651542355	0.0
23	0.0	0.0	0.0	0.06324465480659376	0.0
24	0.0	0.0	0.0	0.07548555573690223	0.0
25	0.0	0.0	0.0	0.07548555573690223	0.0
26	0.0	0.0	0.0	0.08160600620205648	0.0
27	0.0	0.0	0.0	0.12444915945813612	0.0
28	0.0	0.0	0.0	0.16117186224906152	0.0
29	0.0	0.0	0.0	0.20809531581524401	0.0
30	0.0	0.0	0.0	0.26113921984658073	0.0
31	0.0	0.0	0.0	0.312142973722866	0.0
32	0.0	0.0	0.0	0.40598988085523097	0.0
33	0.0	0.0	0.0	0.5324791904684185	0.0
34	0.0	0.0	0.0	0.6732495511669659	0.0
35	0.0	0.0	0.0	0.8833850171372613	0.0
36	0.0	0.0	0.0	1.1608454382242532	0.0
37	0.0	0.0	0.0	1.6892443283825689	0.0
38	0.0	0.0	0.0	2.1217561612534683	0.0
39	0.0	0.0	0.0	2.5440672433491103	0.0
40	0.0	0.0	0.0	2.972498775909907	0.0
41	0.0	0.0	0.0	3.3764485066100867	0.0
42	0.0	0.0	0.0	3.749795984984495	0.0
43	0.0	0.0	0.0	4.1088624122735435	0.0
44	0.0	0.0	0.0	4.529133344214134	0.0
45	0.0	0.0	0.0	4.814754365921332	0.0
46	0.0	0.0	0.0	5.1513791415048145	0.0
47	0.0	0.0	0.0	5.498204667863555	0.0
48	0.0	0.0	0.0	5.761384037865187	0.0
49	0.0	0.0	0.0	6.042924759262282	0.0
50	0.0	0.0	0.0	6.31426472988412	0.0
51	0.0	0.0	0.0	6.5917251509711114	0.0
52	0.0	0.0	0.0	6.8549045209727435	0.0
53	0.0	0.0	0.0	7.187448996246124	0.0
54	0.0	0.0	0.0	7.39962461237147	0.0
55	0.0	0.0	0.0	7.654643381752897	0.0
56	0.0	0.0	0.0	7.8668189978782435	0.0
57	0.0	0.0	0.0	8.089195364778847	0.0
58	0.0	0.0	0.0	8.299330830749144	0.0
59	0.0	0.0	0.0	8.515586747184592	0.0
60	0.0	0.0	0.0	8.707360861759426	0.0
61	0.0	0.0	0.0	8.890974375714052	0.0
62	0.0	0.0	0.0	9.068467439203525	0.0
63	0.0	0.0	0.0	9.356128611065774	0.0
64	0.0	0.0	0.0	9.547902725640608	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACCAT	15	0.00221218	70.0	69
AAACTAT	15	0.00221218	70.0	68
AAACTAC	20	7.85323E-5	70.0	68
ACAGATT	20	7.85323E-5	70.0	33
TTCGTAT	20	7.85323E-5	70.0	39
AAACAGT	15	0.00221218	70.0	69
ATGGCGG	15	0.00221218	70.0	5
AACTACA	15	0.00221218	70.0	69
CAGATTT	15	0.00221218	70.0	34
GATTTCG	15	0.00221218	70.0	36
AAACGTT	15	0.00221218	70.0	69
AGATTTC	15	0.00221218	70.0	35
TCTTTAT	55	0.0	70.0	1
ATTTCGT	15	0.00221218	70.0	37
TTTCGTA	15	0.00221218	70.0	38
TCTTAAT	40	1.3096724E-10	70.0	1
ACTACAA	15	0.00221218	70.0	70
CTTTACA	50	1.2732926E-11	63.000004	1
AAATATA	45	3.7289283E-10	62.222218	68
CTATACA	60	1.8189894E-12	58.333332	1
>>END_MODULE